(Linear) (Six-base) MAPSORT of: KIEE5111.seq  Check: 7894  from: 1  to: 339



KIEE5111.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

ApoI r'AATT_y

Cuts at:      0     101     339 
   Size:        101     238
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     130     339 
   Size:        130     209
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      19      53     339 
   Size:         19      34     286

  Fragments arranged by size: 

                 286      34      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     308     339 
   Size:        308      31
 
BsaWI w'CCGG_w

Cuts at:      0     228     339 
   Size:        228     111
 
BspEI T'CCGG_A

Cuts at:      0     228     339 
   Size:        228     111
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      71     339 
   Size:         71     268
 
BsrDI GCAATG_nn'

Cuts at:      0     213     339 
   Size:        213     126
 
BstEII G'GTnAC_C

Cuts at:      0     259     339 
   Size:        259      80
 
EcoRI G'AATT_C

Cuts at:      0     101     339 
   Size:        101     238
 
MspA1I CmG'CkG

Cuts at:      0     321     339 
   Size:        321      18
 
NdeI CA'TA_TG

Cuts at:      0     277     339 
   Size:        277      62
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     339 
   Size:          7      32     300

  Fragments arranged by size: 

                 300      32       7
 
PvuII CAG'CTG

Cuts at:      0     321     339 
   Size:        321      18
 
SfcI C'TryA_G

Cuts at:      0     253     339 
   Size:        253      86
 
SpeI A'CTAG_T

Cuts at:      0     174     339 
   Size:        174     165
 
StyI C'CwwG_G

Cuts at:      0     141     339 
   Size:        141     198
 
XmnI GAAnn'nnTTC

Cuts at:      0     245     339 
   Size:        245      94
 

 Enzymes that do cut:

     ApoI     BbsI     BcgI   Bpu10I    BsaWI    BspEI    BspMI    BsrDI
   BstEII    EcoRI   MspA1I     NdeI     PpiI    PvuII     SfcI     SpeI
     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI