(Linear) (Six-base) MAPSORT of: KIEE5110.seq  Check: 2987  from: 1  to: 471



KIEE5110.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     471 
   Size:        314     157
 
ApoI r'AATT_y

Cuts at:      0     197     229     471 
   Size:        197      32     242

  Fragments arranged by size: 

                 242     197      32
 
AvaI C'yCGr_G

Cuts at:      0     452     471 
   Size:        452      19
 
BanI G'GyrC_C

Cuts at:      0     393     471 
   Size:        393      78
 
BglII A'GATC_T

Cuts at:      0      35     471 
   Size:         35     436
 
BlpI GC'TnA_GC

Cuts at:      0     353     471 
   Size:        353     118
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     152     471 
   Size:        152     319
 
Bpu10I CC'TnA_GC

Cuts at:      0     335     389     413     471 
   Size:        335      54      24      58

  Fragments arranged by size: 

                 335      58      54      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     471 
   Size:         36     435
 
BsrDI GCAATG_nn'

Cuts at:      0     383     471 
   Size:        383      88
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     259     382     471 
   Size:        259     123      89

  Fragments arranged by size: 

                 259     123      89
 
BstYI r'GATC_y

Cuts at:      0      35     471 
   Size:         35     436
 
EaeI y'GGCC_r

Cuts at:      0     272     471 
   Size:        272     199
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     471 
   Size:        314     157
 
HaeII r_GCGC'y

Cuts at:      0      60     471 
   Size:         60     411
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     471 
   Size:        170     301
 
MscI TGG'CCA

Cuts at:      0     274     471 
   Size:        274     197
 
SfcI C'TryA_G

Cuts at:      0     162     471 
   Size:        162     309
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     471 
   Size:         85     386
 
XmnI GAAnn'nnTTC

Cuts at:      0      82     197     471 
   Size:         82     115     274

  Fragments arranged by size: 

                 274     115      82
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BanI    BglII     BlpI     BplI   Bpu10I
     BsgI    BsrDI   BstAPI    BstYI     EaeI  Eco57MI    HaeII     MmeI
     MscI     SfcI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI