(Linear) (Six-base) MAPSORT of: KIEE5109.seq  Check: 7112  from: 1  to: 477



KIEE5109.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     477 
   Size:         88     389
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     383     477 
   Size:        383      94
 
AvrII C'CTAG_G

Cuts at:      0      39     477 
   Size:         39     438
 
BanI G'GyrC_C

Cuts at:      0      34     477 
   Size:         34     443
 
BanII G_rGCy'C

Cuts at:      0      54     477 
   Size:         54     423
 
Bme1580I G_kGCm'C

Cuts at:      0      39     477 
   Size:         39     438
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     321     477 
   Size:        321     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      53     477 
   Size:         33      20     424

  Fragments arranged by size: 

                 424      33      20
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       6      36     477 
   Size:          6      30     441

  Fragments arranged by size: 

                 441      30       6
 
BseYI C'CCAG_C

Cuts at:      0     409     477 
   Size:        409      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     477 
   Size:        157     320
 
BsiHKAI G_wGCw'C

Cuts at:      0      54     477 
   Size:         54     423
 
Bsp1286I G_dGCh'C

Cuts at:      0      39      54     477 
   Size:         39      15     423

  Fragments arranged by size: 

                 423      39      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      90     477 
   Size:         90     387
 
BstBI TT'CG_AA

Cuts at:      0      63     477 
   Size:         63     414
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     169     477 
   Size:        169     308
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     283     477 
   Size:        283     194
 
DraIII CAC_nnn'GTG

Cuts at:      0     438     477 
   Size:        438      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     305     477 
   Size:        305     172
 
EcoICRI GAG'CTC

Cuts at:      0      52     477 
   Size:         52     425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      53      88     477 
   Size:         33      20      35     389

  Fragments arranged by size: 

                 389      35      33      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     315     477 
   Size:        315     162
 
MslI CAynn'nnrTG

Cuts at:      0     350     477 
   Size:        350     127
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     467     477 
   Size:        467      10
 
PstI C_TGCA'G

Cuts at:      0     291     477 
   Size:        291     186
 
SacI G_AGCT'C

Cuts at:      0      54     477 
   Size:         54     423
 
SfcI C'TryA_G

Cuts at:      0     287     477 
   Size:        287     190
 
StyI C'CwwG_G

Cuts at:      0      39     176     318     371     477 
   Size:         39     137     142      53     106

  Fragments arranged by size: 

                 142     137     106      53      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125     477 
   Size:        125     352
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       6      38     477 
   Size:          6      32     439

  Fragments arranged by size: 

                 439      32       6
 

 Enzymes that do cut:

     AcuI     AlfI    AvrII     BanI    BanII Bme1580I     BmrI     BpmI
    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BstBI    BstXI
    BtgZI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I     MslI    PflMI
     PstI     SacI     SfcI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI