(Linear) (Six-base) MAPSORT of: KIEE5108.seq  Check: 25  from: 1  to: 414



KIEE5108.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AfeI AGC'GCT

Cuts at:      0     402     414 
   Size:        402      12
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     150     414 
   Size:         27     123     264

  Fragments arranged by size: 

                 264     123      27
 
ApaLI G'TGCA_C

Cuts at:      0     156     414 
   Size:        156     258
 
ApoI r'AATT_y

Cuts at:      0      87     414 
   Size:         87     327
 
AvaI C'yCGr_G

Cuts at:      0     136     223     414 
   Size:        136      87     191

  Fragments arranged by size: 

                 191     136      87
 
BanI G'GyrC_C

Cuts at:      0     378     414 
   Size:        378      36
 
BanII G_rGCy'C

Cuts at:      0     232     241     414 
   Size:        232       9     173

  Fragments arranged by size: 

                 232     173       9
 
BlpI GC'TnA_GC

Cuts at:      0     190     414 
   Size:        190     224
 
Bme1580I G_kGCm'C

Cuts at:      0     160     414 
   Size:        160     254
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     328     358     414 
   Size:        328      30      56

  Fragments arranged by size: 

                 328      56      30
 
BseYI C'CCAG_C

Cuts at:      0     242     403     414 
   Size:        242     161      11

  Fragments arranged by size: 

                 242     161      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     414 
   Size:        328      86
 
BsiHKAI G_wGCw'C

Cuts at:      0     160     232     414 
   Size:        160      72     182

  Fragments arranged by size: 

                 182     160      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     232     241     414 
   Size:        160      72       9     173

  Fragments arranged by size: 

                 173     160      72       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     332     414 
   Size:        332      82
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      27     150     414 
   Size:         27     123     264

  Fragments arranged by size: 

                 264     123      27
 
BstYI r'GATC_y

Cuts at:      0     278     414 
   Size:        278     136
 
BtgI C'CryG_G

Cuts at:      0     390     414 
   Size:        390      24
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      33      68     414 
   Size:         33      35     346

  Fragments arranged by size: 

                 346      35      33
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     210     414 
   Size:        210     204
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     249     414 
   Size:        249     165
 
EcoICRI GAG'CTC

Cuts at:      0     230     414 
   Size:        230     184
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     321     414 
   Size:        203     118      93

  Fragments arranged by size: 

                 203     118      93
 
EcoRI G'AATT_C

Cuts at:      0      87     414 
   Size:         87     327
 
HaeII r_GCGC'y

Cuts at:      0     404     414 
   Size:        404      10
 
MspA1I CmG'CkG

Cuts at:      0     175     312     414 
   Size:        175     137     102

  Fragments arranged by size: 

                 175     137     102
 
PasI CC'CwG_GG

Cuts at:      0     120     291     414 
   Size:        120     171     123

  Fragments arranged by size: 

                 171     123     120
 
PfoI T'CCnGG_A

Cuts at:      0     232     414 
   Size:        232     182
 
PpuMI rG'GwC_Cy

Cuts at:      0     203     321     414 
   Size:        203     118      93

  Fragments arranged by size: 

                 203     118      93
 
PvuII CAG'CTG

Cuts at:      0     175     414 
   Size:        175     239
 
SacI G_AGCT'C

Cuts at:      0     232     414 
   Size:        232     182
 
SmaI CCC'GGG

Cuts at:      0     138     414 
   Size:        138     276
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61     414 
   Size:         61     353
 
XbaI T'CTAG_A

Cuts at:      0      91     414 
   Size:         91     323
 
XmaI C'CCGG_G

Cuts at:      0     136     414 
   Size:        136     278
 
XmnI GAAnn'nnTTC

Cuts at:      0     346     414 
   Size:        346      68
 

 Enzymes that do cut:

     AfeI    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII     BlpI
 Bme1580I    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstYI     BtgI    CspCI     DrdI     EciI  EcoICRI EcoO109I    EcoRI
    HaeII   MspA1I     PasI     PfoI    PpuMI    PvuII     SacI     SmaI
    TaqII     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     EaeI     EagI     EarI  Eco57MI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     ZraI