(Linear) (Six-base) MAPSORT of: KIEE5107.seq  Check: 3922  from: 1  to: 309



KIEE5107.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     309 
   Size:        130     179
 
AleI CACnn'nnGTG

Cuts at:      0     238     309 
   Size:        238      71
 
BanI G'GyrC_C

Cuts at:      0     148     309 
   Size:        148     161
 
Bme1580I G_kGCm'C

Cuts at:      0     235     309 
   Size:        235      74
 
BmgBI CAC'GTC

Cuts at:      0     236     309 
   Size:        236      73
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     161     309 
   Size:        161     148
 
BsaHI Gr'CG_yC

Cuts at:      0     305     309 
   Size:        305       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     309 
   Size:        118     191
 
BseYI C'CCAG_C

Cuts at:      0     210     309 
   Size:        210      99
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     309 
   Size:        235      74
 
BssSI C'ACGA_G

Cuts at:      0     217     309 
   Size:        217      92
 
BtgI C'CryG_G

Cuts at:      0     239     309 
   Size:        239      70
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     309 
   Size:         19     290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     309 
   Size:        130     179
 
MslI CAynn'nnrTG

Cuts at:      0     238     309 
   Size:        238      71
 
MspA1I CmG'CkG

Cuts at:      0     210     309 
   Size:        210      99
 
NspI r_CATG'y

Cuts at:      0     165     309 
   Size:        165     144
 
PvuII CAG'CTG

Cuts at:      0     210     309 
   Size:        210      99
 
TatI w'GTAC_w

Cuts at:      0      50     158     182     309 
   Size:         50     108      24     127

  Fragments arranged by size: 

                 127     108      50      24
 
XmnI GAAnn'nnTTC

Cuts at:      0     115     309 
   Size:        115     194
 

 Enzymes that do cut:

     AcuI     AleI     BanI Bme1580I    BmgBI     BsaI    BsaHI    BseRI
    BseYI Bsp1286I    BssSI     BtgI     EciI  Eco57MI     MslI   MspA1I
     NspI    PvuII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI