(Linear) (Six-base) MAPSORT of: KIEE5105.seq  Check: 7977  from: 1  to: 345



KIEE5105.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     345 
   Size:        182     163
 
ApoI r'AATT_y

Cuts at:      0     112     345 
   Size:        112     233
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     165     345 
   Size:        165     180
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      43      77     345 
   Size:         43      34     268

  Fragments arranged by size: 

                 268      43      34
 
Bme1580I G_kGCm'C

Cuts at:      0      81      92     345 
   Size:         81      11     253

  Fragments arranged by size: 

                 253      81      11
 
BmgBI CAC'GTC

Cuts at:      0     325     345 
   Size:        325      20
 
BsaAI yAC'GTr

Cuts at:      0      51     345 
   Size:         51     294
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51      73     345 
   Size:         51      22     272

  Fragments arranged by size: 

                 272      51      22
 
Bsp1286I G_dGCh'C

Cuts at:      0      81      92     345 
   Size:         81      11     253

  Fragments arranged by size: 

                 253      81      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     315     345 
   Size:        315      30
 
BssSI C'ACGA_G

Cuts at:      0     263     345 
   Size:        263      82
 
BstBI TT'CG_AA

Cuts at:      0     123     345 
   Size:        123     222
 
BtgI C'CryG_G

Cuts at:      0      42     345 
   Size:         42     303
 
ClaI AT'CG_AT

Cuts at:      0     258     345 
   Size:        258      87
 
EaeI y'GGCC_r

Cuts at:      0      45     345 
   Size:         45     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     345 
   Size:        182     163
 
MslI CAynn'nnrTG

Cuts at:      0     218     345 
   Size:        218     127
 
MspA1I CmG'CkG

Cuts at:      0      44     345 
   Size:         44     301
 
PmlI CAC'GTG

Cuts at:      0      51     345 
   Size:         51     294
 
SacII CC_GC'GG

Cuts at:      0      45     345 
   Size:         45     300
 
StyI C'CwwG_G

Cuts at:      0      92     345 
   Size:         92     253
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     296     345 
   Size:        296      49
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI     BcgI Bme1580I    BmgBI    BsaAI     BsgI
 Bsp1286I    BspMI    BssSI    BstBI     BtgI     ClaI     EaeI  Eco57MI
     MslI   MspA1I     PmlI    SacII     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI