(Linear) (Six-base) MAPSORT of: KIEE5104.seq  Check: 3056  from: 1  to: 351



KIEE5104.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AflIII A'CryG_T

Cuts at:      0     216     351 
   Size:        216     135
 
Bme1580I G_kGCm'C

Cuts at:      0     148     351 
   Size:        148     203
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     351 
   Size:        307      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     351 
   Size:        148     203
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     289     351 
   Size:        289      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     227     351 
   Size:        227     124
 
NspI r_CATG'y

Cuts at:      0     137     220     351 
   Size:        137      83     131

  Fragments arranged by size: 

                 137     131      83
 
PciI A'CATG_T

Cuts at:      0     216     351 
   Size:        216     135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     261     293     351 
   Size:        261      32      58

  Fragments arranged by size: 

                 261      58      32
 

 Enzymes that do cut:

   AflIII Bme1580I    BseRI Bsp1286I    EcoNI EcoO109I     NspI     PciI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI