(Linear) (Six-base) MAPSORT of: KIEE5101.seq  Check: 7923  from: 1  to: 285



KIEE5101.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     167     285 
   Size:        167     118
 
AlwNI CAG_nnn'CTG

Cuts at:      0     108     285 
   Size:        108     177
 
ApaI G_GGCC'C

Cuts at:      0     213     285 
   Size:        213      72
 
AvaI C'yCGr_G

Cuts at:      0     206     285 
   Size:        206      79
 
BamHI G'GATC_C

Cuts at:      0     155     285 
   Size:        155     130
 
BanII G_rGCy'C

Cuts at:      0     213     269     285 
   Size:        213      56      16

  Fragments arranged by size: 

                 213      56      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       9     285 
   Size:          9     276
 
Bme1580I G_kGCm'C

Cuts at:      0     213     285 
   Size:        213      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     207     285 
   Size:         77     130      78

  Fragments arranged by size: 

                 130      78      77
 
Bsp1286I G_dGCh'C

Cuts at:      0     213     269     285 
   Size:        213      56      16

  Fragments arranged by size: 

                 213      56      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     285 
   Size:        167     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     285 
   Size:         48     237
 
BstYI r'GATC_y

Cuts at:      0     155     285 
   Size:        155     130
 
Bsu36I CC'TnA_GG

Cuts at:      0     109     285 
   Size:        109     176
 
BtgI C'CryG_G

Cuts at:      0      41     285 
   Size:         41     244
 
EaeI y'GGCC_r

Cuts at:      0      50     285 
   Size:         50     235
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     285 
   Size:        148     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210     285 
   Size:        210      75
 
MscI TGG'CCA

Cuts at:      0      52     285 
   Size:         52     233
 
MspA1I CmG'CkG

Cuts at:      0      61     285 
   Size:         61     224
 
NcoI C'CATG_G

Cuts at:      0      41     285 
   Size:         41     244
 
PspOMI G'GGCC_C

Cuts at:      0     209     285 
   Size:        209      76
 
PstI C_TGCA'G

Cuts at:      0      28     285 
   Size:         28     257
 
PvuII CAG'CTG

Cuts at:      0      61     285 
   Size:         61     224
 
SfcI C'TryA_G

Cuts at:      0      24     285 
   Size:         24     261
 
SmaI CCC'GGG

Cuts at:      0     208     285 
   Size:        208      77
 
StyI C'CwwG_G

Cuts at:      0      41     285 
   Size:         41     244
 
XmaI C'CCGG_G

Cuts at:      0     206     285 
   Size:        206      79
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     AvaI    BamHI    BanII     BbsI Bme1580I
     BsgI Bsp1286I    BspMI    BstXI    BstYI   Bsu36I     BtgI     EaeI
     EarI EcoO109I     MscI   MspA1I     NcoI   PspOMI     PstI    PvuII
     SfcI     SmaI     StyI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI