(Linear) (Six-base) MAPSORT of: KIEE5087.seq  Check: 8391  from: 1  to: 237



KIEE5087.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

BanI G'GyrC_C

Cuts at:      0     112     193     237 
   Size:        112      81      44

  Fragments arranged by size: 

                 112      81      44
 
BsaBI GATnn'nnATC

Cuts at:      0     226     237 
   Size:        226      11
 
BseYI C'CCAG_C

Cuts at:      0      32     237 
   Size:         32     205
 
BsiEI CG_ry'CG

Cuts at:      0      51      98     237 
   Size:         51      47     139

  Fragments arranged by size: 

                 139      51      47
 
BsmI GAATG_Cn'

Cuts at:      0     143     237 
   Size:        143      94
 
BsrFI r'CCGG_y

Cuts at:      0       3     237 
   Size:          3     234
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     233     237 
   Size:        233       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     222     237 
   Size:        222      15
 
BtgI C'CryG_G

Cuts at:      0      11      83     237 
   Size:         11      72     154

  Fragments arranged by size: 

                 154      72      11
 
EaeI y'GGCC_r

Cuts at:      0       8      95     212     237 
   Size:          8      87     117      25

  Fragments arranged by size: 

                 117      87      25       8
 
EagI C'GGCC_G

Cuts at:      0      95     237 
   Size:         95     142
 
EarI CTCTTCn'nnn_

Cuts at:      0     112     237 
   Size:        112     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     126     237 
   Size:        126     111
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     237 
   Size:         16     221
 
HaeII r_GCGC'y

Cuts at:      0     106     237 
   Size:        106     131
 
MscI TGG'CCA

Cuts at:      0     214     237 
   Size:        214      23
 
MslI CAynn'nnrTG

Cuts at:      0     208     220     237 
   Size:        208      12      17

  Fragments arranged by size: 

                 208      17      12
 
PpuMI rG'GwC_Cy

Cuts at:      0      16     237 
   Size:         16     221
 
SanDI GG'GwC_CC

Cuts at:      0      16     237 
   Size:         16     221
 
SapI GCTCTTCn'nnn_

Cuts at:      0     112     237 
   Size:        112     125
 
Tth111I GACn'n_nGTC

Cuts at:      0      52     237 
   Size:         52     185
 

 Enzymes that do cut:

     BanI    BsaBI    BseYI    BsiEI     BsmI    BsrFI   BstAPI    BstXI
     BtgI     EaeI     EagI     EarI    EcoNI EcoO109I    HaeII     MscI
     MslI    PpuMI    SanDI     SapI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI