(Linear) (Six-base) MAPSORT of: KIEE5086.seq  Check: 6301  from: 1  to: 237



KIEE5086.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     223     237 
   Size:         60     163      14

  Fragments arranged by size: 

                 163      60      14
 
AvaI C'yCGr_G

Cuts at:      0      55     237 
   Size:         55     182
 
BanII G_rGCy'C

Cuts at:      0      64     237 
   Size:         64     173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32     237 
   Size:         32     205
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     152     237 
   Size:         44     108      85

  Fragments arranged by size: 

                 108      85      44
 
BmgBI CAC'GTC

Cuts at:      0     198     237 
   Size:        198      39
 
BsaWI w'CCGG_w

Cuts at:      0     185     229     237 
   Size:        185      44       8

  Fragments arranged by size: 

                 185      44       8
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      85     115     237 
   Size:         85      30     122

  Fragments arranged by size: 

                 122      85      30
 
BseYI C'CCAG_C

Cuts at:      0     141     237 
   Size:        141      96
 
BsiEI CG_ry'CG

Cuts at:      0     156     237 
   Size:        156      81
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     237 
   Size:         64     173
 
BspEI T'CCGG_A

Cuts at:      0     229     237 
   Size:        229       8
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     237 
   Size:        168      69
 
BstYI r'GATC_y

Cuts at:      0       8     237 
   Size:          8     229
 
EaeI y'GGCC_r

Cuts at:      0     153     237 
   Size:        153      84
 
EagI C'GGCC_G

Cuts at:      0     153     237 
   Size:        153      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     223     237 
   Size:         60     163      14

  Fragments arranged by size: 

                 163      60      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117     158     190     237 
   Size:         85      32      41      32      47

  Fragments arranged by size: 

                  85      47      41      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     237 
   Size:         89     148
 
MspA1I CmG'CkG

Cuts at:      0      80     237 
   Size:         80     157
 
PstI C_TGCA'G

Cuts at:      0      92     237 
   Size:         92     145
 
PvuII CAG'CTG

Cuts at:      0      80     237 
   Size:         80     157
 
SbfI CC_TGCA'GG

Cuts at:      0      92     237 
   Size:         92     145
 
SfcI C'TryA_G

Cuts at:      0      18      88     237 
   Size:         18      70     149

  Fragments arranged by size: 

                 149      70      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     152     237 
   Size:        152      85
 

 Enzymes that do cut:

     AcuI     AvaI    BanII     BbsI     BglI    BmgBI    BsaWI    BsaXI
    BseYI    BsiEI Bsp1286I    BspEI    BstXI    BstYI     EaeI     EagI
  Eco57MI    Hin4I     MmeI   MspA1I     PstI    PvuII     SbfI     SfcI
     SfiI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI