(Linear) (Six-base) MAPSORT of: KIEE5085.seq  Check: 7696  from: 1  to: 183



KIEE5085.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     183 
   Size:        162      21
 
AfeI AGC'GCT

Cuts at:      0       7     183 
   Size:          7     176
 
BbvCI CC'TCA_GC

Cuts at:      0     105     183 
   Size:        105      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     105     183 
   Size:        105      78
 
EaeI y'GGCC_r

Cuts at:      0     149     183 
   Size:        149      34
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     175     183 
   Size:         19     156       8

  Fragments arranged by size: 

                 156      19       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     183 
   Size:        162      21
 
HaeII r_GCGC'y

Cuts at:      0       9     183 
   Size:          9     174
 
MscI TGG'CCA

Cuts at:      0     151     183 
   Size:        151      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     183 
   Size:         36     147
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     183 
   Size:          7     176
 

 Enzymes that do cut:

     AcuI     AfeI    BbvCI   Bpu10I     EaeI     EarI  Eco57MI    HaeII
     MscI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI