Restriction map for
pFN21AE5083
(Linear) (Six-base) MAPSORT of: KIEE5083.seq Check: 6794 from: 1 to: 2931
KIEE5083.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1048 2931
Size: 1048 1883
AatII G_ACGT'C
Cuts at: 0 1953 2931
Size: 1953 978
AccI GT'mk_AC
Cuts at: 0 1571 2931
Size: 1571 1360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 754 2308 2401 2849 2931
Size: 535 219 1554 93 448 82
Fragments arranged by size:
1554 535 448 219 93 82
AfeI AGC'GCT
Cuts at: 0 148 2931
Size: 148 2783
AgeI A'CCGG_T
Cuts at: 0 340 2931
Size: 340 2591
AhdI GACnn_n'nnGTC
Cuts at: 0 2607 2931
Size: 2607 324
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1379 1411 2659 2691 2931
Size: 1379 32 1248 32 240
Fragments arranged by size:
1379 1248 240 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1063 1611 1620 2326 2638 2796 2931
Size: 1063 548 9 706 312 158 135
Fragments arranged by size:
1063 706 548 312 158 135 9
ApaI G_GGCC'C
Cuts at: 0 2561 2931
Size: 2561 370
ApaLI G'TGCA_C
Cuts at: 0 2255 2931
Size: 2255 676
ApoI r'AATT_y
Cuts at: 0 396 1258 1420 2931
Size: 396 862 162 1511
Fragments arranged by size:
1511 862 396 162
AvaI C'yCGr_G
Cuts at: 0 384 431 971 1096 1691 2931
Size: 384 47 540 125 595 1240
Fragments arranged by size:
1240 595 540 384 125 47
AvrII C'CTAG_G
Cuts at: 0 1915 2931
Size: 1915 1016
BanI G'GyrC_C
Cuts at: 0 761 842 1435 1668 1758 2931
Size: 761 81 593 233 90 1173
Fragments arranged by size:
1173 761 593 233 90 81
BanII G_rGCy'C
Cuts at: 0 229 2561 2931
Size: 229 2332 370
Fragments arranged by size:
2332 370 229
BbeI G_GCGC'C
Cuts at: 0 765 2931
Size: 765 2166
BbsI GAAGACnn'nnnn_
Cuts at: 0 2071 2243 2437 2931
Size: 2071 172 194 494
Fragments arranged by size:
2071 494 194 172
BbvCI CC'TCA_GC
Cuts at: 0 474 2622 2931
Size: 474 2148 309
Fragments arranged by size:
2148 474 309
BciVI GTATCCnnnnn_n'
Cuts at: 0 895 2931
Size: 895 2036
BclI T'GATC_A
Cuts at: 0 1104 2708 2849 2931
Size: 1104 1604 141 82
Fragments arranged by size:
1604 1104 141 82
BfrBI ATG'CAT
Cuts at: 0 2409 2931
Size: 2409 522
BglI GCCn_nnn'nGGC
Cuts at: 0 2329 2931
Size: 2329 602
BglII A'GATC_T
Cuts at: 0 1890 2931
Size: 1890 1041
Bme1580I G_kGCm'C
Cuts at: 0 1671 2259 2561 2931
Size: 1671 588 302 370
Fragments arranged by size:
1671 588 370 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 1768 2931
Size: 1768 1163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 1241 1706 2931
Size: 130 1111 465 1225
Fragments arranged by size:
1225 1111 465 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 872 1000 1073 2486 2931
Size: 339 533 128 73 1413 445
Fragments arranged by size:
1413 533 445 339 128 73
Bpu10I CC'TnA_GC
Cuts at: 0 474 2622 2931
Size: 474 2148 309
Fragments arranged by size:
2148 474 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 2470 2931
Size: 1054 1416 461
Fragments arranged by size:
1416 1054 461
BsaI GGTCTCn'nnnn_
Cuts at: 0 968 1901 1970 2144 2931
Size: 968 933 69 174 787
Fragments arranged by size:
968 933 787 174 69
BsaBI GATnn'nnATC
Cuts at: 0 794 1889 2931
Size: 794 1095 1042
Fragments arranged by size:
1095 1042 794
BsaHI Gr'CG_yC
Cuts at: 0 762 1560 1950 2931
Size: 762 798 390 981
Fragments arranged by size:
981 798 762 390
BsaWI w'CCGG_w
Cuts at: 0 340 1118 2192 2931
Size: 340 778 1074 739
Fragments arranged by size:
1074 778 739 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 783 813 2187 2217 2459 2489 2931
Size: 783 30 1374 30 242 30 442
Fragments arranged by size:
1374 783 442 242 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 462 1951 2604 2931
Size: 462 1489 653 327
Fragments arranged by size:
1489 653 462 327
BseYI C'CCAG_C
Cuts at: 0 536 1897 2480 2786 2931
Size: 536 1361 583 306 145
Fragments arranged by size:
1361 583 536 306 145
BsiHKAI G_wGCw'C
Cuts at: 0 391 719 1692 2259 2931
Size: 391 328 973 567 672
Fragments arranged by size:
973 672 567 391 328
BsmBI CGTCTCn'nnnn_
Cuts at: 0 101 2506 2931
Size: 101 2405 425
Fragments arranged by size:
2405 425 101
Bsp1286I G_dGCh'C
Cuts at: 0 229 391 719 1671 1692 2259 2561 2931
Size: 229 162 328 952 21 567 302 370
Fragments arranged by size:
952 567 370 328 302 229 162 21
BspHI T'CATG_A
Cuts at: 0 573 2846 2931
Size: 573 2273 85
Fragments arranged by size:
2273 573 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1048 2931
Size: 1048 1883
BsrBI CCG'CTC
Cuts at: 0 207 645 773 2243 2931
Size: 207 438 128 1470 688
Fragments arranged by size:
1470 688 438 207 128
BsrDI GCAATG_nn'
Cuts at: 0 246 964 1304 2931
Size: 246 718 340 1627
Fragments arranged by size:
1627 718 340 246
BsrFI r'CCGG_y
Cuts at: 0 340 725 758 839 914 2606 2931
Size: 340 385 33 81 75 1692 325
Fragments arranged by size:
1692 385 340 325 81 75 33
BssSI C'ACGA_G
Cuts at: 0 1415 2931
Size: 1415 1516
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1063 1510 1611 1620 2370 2415 2931
Size: 1063 447 101 9 750 45 516
Fragments arranged by size:
1063 750 516 447 101 45 9
BstEII G'GTnAC_C
Cuts at: 0 835 2422 2931
Size: 835 1587 509
Fragments arranged by size:
1587 835 509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2580 2817 2931
Size: 2580 237 114
Fragments arranged by size:
2580 237 114
BstYI r'GATC_y
Cuts at: 0 1890 2931
Size: 1890 1041
Bsu36I CC'TnA_GG
Cuts at: 0 1934 2931
Size: 1934 997
BtgI C'CryG_G
Cuts at: 0 28 102 174 198 2859 2931
Size: 28 74 72 24 2661 72
Fragments arranged by size:
2661 74 72 72 28 24
BtsI GCAGTG_nn'
Cuts at: 0 1995 2270 2636 2931
Size: 1995 275 366 295
Fragments arranged by size:
1995 366 295 275
ClaI AT'CG_AT
Cuts at: 0 1428 2067 2931
Size: 1428 639 864
Fragments arranged by size:
1428 864 639
DraI TTT'AAA
Cuts at: 0 2684 2931
Size: 2684 247
DraIII CAC_nnn'GTG
Cuts at: 0 1546 2776 2931
Size: 1546 1230 155
Fragments arranged by size:
1546 1230 155
EaeI y'GGCC_r
Cuts at: 0 670 689 936 1230 2819 2931
Size: 670 19 247 294 1589 112
Fragments arranged by size:
1589 670 294 247 112 19
EarI CTCTTCn'nnn_
Cuts at: 0 1132 2125 2622 2896 2931
Size: 1132 993 497 274 35
Fragments arranged by size:
1132 993 497 274 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 48 300 947 2931
Size: 48 252 647 1984
Fragments arranged by size:
1984 647 252 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 535 754 872 1000 1073 2308 2401
Size: 339 196 219 118 128 73 1235 93
Cuts at: 2401 2486 2849 2931
Size: 85 363 82
Fragments arranged by size:
1235 363 339 219 196 128 118 93
85 82 73
EcoO109I rG'GnC_Cy
Cuts at: 0 18 533 821 1387 1734 2093 2557 2754
Size: 18 515 288 566 347 359 464 197
Cuts at: 2754 2931
Size: 177
Fragments arranged by size:
566 515 464 359 347 288 197 177
18
EcoRV GAT'ATC
Cuts at: 0 1212 2931
Size: 1212 1719
HaeII r_GCGC'y
Cuts at: 0 150 765 893 2920 2931
Size: 150 615 128 2027 11
Fragments arranged by size:
2027 615 150 128 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1846 1878 2187 2219 2593 2625
Size: 517 32 1297 32 309 32 374 32
Cuts at: 2625 2931
Size: 306
Fragments arranged by size:
1297 517 374 309 306 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 761 2931
Size: 761 2170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 2426 2504 2931
Size: 141 2285 78 427
Fragments arranged by size:
2285 427 141 78
MscI TGG'CCA
Cuts at: 0 2821 2931
Size: 2821 110
MspA1I CmG'CkG
Cuts at: 0 30 63 104 200 254 635 2173 2626
Size: 30 33 41 96 54 381 1538 453
Cuts at: 2626 2931
Size: 305
Fragments arranged by size:
1538 453 381 305 96 54 41 33
30
NaeI GCC'GGC
Cuts at: 0 760 2931
Size: 760 2171
NarI GG'CG_CC
Cuts at: 0 762 2931
Size: 762 2169
NdeI CA'TA_TG
Cuts at: 0 1526 2931
Size: 1526 1405
NgoMIV G'CCGG_C
Cuts at: 0 758 2931
Size: 758 2173
NsiI A_TGCA'T
Cuts at: 0 2411 2931
Size: 2411 520
NspI r_CATG'y
Cuts at: 0 700 1513 2373 2409 2931
Size: 700 813 860 36 522
Fragments arranged by size:
860 813 700 522 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 1546 2931
Size: 1546 1385
PfoI T'CCnGG_A
Cuts at: 0 2795 2931
Size: 2795 136
PpuMI rG'GwC_Cy
Cuts at: 0 18 533 1387 1734 2754 2931
Size: 18 515 854 347 1020 177
Fragments arranged by size:
1020 854 515 347 177 18
PshAI GACnn'nnGTC
Cuts at: 0 2018 2931
Size: 2018 913
PsiI TTA'TAA
Cuts at: 0 1315 2931
Size: 1315 1616
PspOMI G'GGCC_C
Cuts at: 0 2557 2931
Size: 2557 374
PstI C_TGCA'G
Cuts at: 0 923 1616 1803 2931
Size: 923 693 187 1128
Fragments arranged by size:
1128 923 693 187
PvuII CAG'CTG
Cuts at: 0 2173 2626 2931
Size: 2173 453 305
Fragments arranged by size:
2173 453 305
SacII CC_GC'GG
Cuts at: 0 31 105 201 2931
Size: 31 74 96 2730
Fragments arranged by size:
2730 96 74 31
SanDI GG'GwC_CC
Cuts at: 0 18 533 2931
Size: 18 515 2398
Fragments arranged by size:
2398 515 18
SapI GCTCTTCn'nnn_
Cuts at: 0 1132 2125 2931
Size: 1132 993 806
Fragments arranged by size:
1132 993 806
ScaI AGT'ACT
Cuts at: 0 1242 2931
Size: 1242 1689
SexAI A'CCwGG_T
Cuts at: 0 2840 2931
Size: 2840 91
SfcI C'TryA_G
Cuts at: 0 827 919 1612 1799 2931
Size: 827 92 693 187 1132
Fragments arranged by size:
1132 827 693 187 92
SfoI GGC'GCC
Cuts at: 0 763 2931
Size: 763 2168
SmaI CCC'GGG
Cuts at: 0 433 2931
Size: 433 2498
SmlI C'TyrA_G
Cuts at: 0 971 1033 2485 2931
Size: 971 62 1452 446
Fragments arranged by size:
1452 971 446 62
SpeI A'CTAG_T
Cuts at: 0 1237 2931
Size: 1237 1694
SphI G_CATG'C
Cuts at: 0 700 1513 2373 2931
Size: 700 813 860 558
Fragments arranged by size:
860 813 700 558
StuI AGG'CCT
Cuts at: 0 1221 1382 2931
Size: 1221 161 1549
Fragments arranged by size:
1549 1221 161
StyI C'CwwG_G
Cuts at: 0 1039 1390 1865 1915 2810 2931
Size: 1039 351 475 50 895 121
Fragments arranged by size:
1039 895 475 351 121 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 1362 1371 1746 2931
Size: 277 1085 9 375 1185
Fragments arranged by size:
1185 1085 375 277 9
TatI w'GTAC_w
Cuts at: 0 1240 2931
Size: 1240 1691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 2459 2491 2931
Size: 1760 32 667 32 440
Fragments arranged by size:
1760 667 440 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 2308 2352 2931
Size: 708 1600 44 579
Fragments arranged by size:
1600 708 579 44
XhoI C'TCGA_G
Cuts at: 0 971 2931
Size: 971 1960
XmaI C'CCGG_G
Cuts at: 0 431 2931
Size: 431 2500
ZraI GAC'GTC
Cuts at: 0 1951 2931
Size: 1951 980
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I
KasI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PflMI PfoI PpuMI PshAI PsiI PspOMI
PstI PvuII SacII SanDI SapI ScaI SexAI SfcI
SfoI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AleI AlfI AscI AseI
AsiSI BaeI BamHI BcgI BlpI BmgBI BmtI BsaAI
BsgI BsiEI BsiWI BsmI BspEI BsrGI BssHII BstBI
BstZ17I BtgZI CspCI DrdI EagI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NcoI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PspXI PsrI PvuI
RsrII SacI SalI SbfI SfiI SgfI SgrAI SnaBI
SrfI SspI SwaI Tth111I XbaI XmnI