Restriction map for
pFN21AE5079
(Linear) (Six-base) MAPSORT of: KIEE5079.seq Check: 4828 from: 1 to: 1077
KIEE5079.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 1077
Size: 727 350
AccI GT'mk_AC
Cuts at: 0 826 1077
Size: 826 251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 350 1077
Size: 206 144 727
Fragments arranged by size:
727 206 144
AleI CACnn'nnGTG
Cuts at: 0 739 1077
Size: 739 338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 934 1077
Size: 934 143
AlwNI CAG_nnn'CTG
Cuts at: 0 328 1077
Size: 328 749
ApoI r'AATT_y
Cuts at: 0 849 1077
Size: 849 228
AvaI C'yCGr_G
Cuts at: 0 693 1077
Size: 693 384
BanI G'GyrC_C
Cuts at: 0 1059 1077
Size: 1059 18
BanII G_rGCy'C
Cuts at: 0 75 442 986 1077
Size: 75 367 544 91
Fragments arranged by size:
544 367 91 75
BbeI G_GCGC'C
Cuts at: 0 1063 1077
Size: 1063 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 633 1077
Size: 294 339 444
Fragments arranged by size:
444 339 294
BbvCI CC'TCA_GC
Cuts at: 0 923 1077
Size: 923 154
BclI T'GATC_A
Cuts at: 0 143 1077
Size: 143 934
BlpI GC'TnA_GC
Cuts at: 0 1000 1077
Size: 1000 77
Bme1580I G_kGCm'C
Cuts at: 0 236 1077
Size: 236 841
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 1077
Size: 460 617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 561 1077
Size: 265 296 516
Fragments arranged by size:
516 296 265
Bpu10I CC'TnA_GC
Cuts at: 0 296 676 759 923 1077
Size: 296 380 83 164 154
Fragments arranged by size:
380 296 164 154 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 948 1077
Size: 948 129
BsaHI Gr'CG_yC
Cuts at: 0 1060 1077
Size: 1060 17
BsaWI w'CCGG_w
Cuts at: 0 1008 1077
Size: 1008 69
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 541 1077
Size: 28 513 536
Fragments arranged by size:
536 513 28
BseYI C'CCAG_C
Cuts at: 0 621 1077
Size: 621 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 913 1077
Size: 841 72 164
Fragments arranged by size:
841 164 72
BsmI GAATG_Cn'
Cuts at: 0 1004 1077
Size: 1004 73
Bsp1286I G_dGCh'C
Cuts at: 0 75 236 442 986 1077
Size: 75 161 206 544 91
Fragments arranged by size:
544 206 161 91 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 1077
Size: 727 350
BssHII G'CGCG_C
Cuts at: 0 1053 1077
Size: 1053 24
BssSI C'ACGA_G
Cuts at: 0 139 1077
Size: 139 938
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 1077
Size: 243 834
BstZ17I GTA'TAC
Cuts at: 0 827 1077
Size: 827 250
BtgI C'CryG_G
Cuts at: 0 1064 1077
Size: 1064 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 573 1077
Size: 573 504
BtsI GCAGTG_nn'
Cuts at: 0 317 734 746 1077
Size: 317 417 12 331
Fragments arranged by size:
417 331 317 12
DraIII CAC_nnn'GTG
Cuts at: 0 432 1077
Size: 432 645
EarI CTCTTCn'nnn_
Cuts at: 0 225 946 1077
Size: 225 721 131
Fragments arranged by size:
721 225 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1077
Size: 760 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 265 350 561 1077
Size: 206 59 85 211 516
Fragments arranged by size:
516 211 206 85 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 1077
Size: 295 782
EcoO109I rG'GnC_Cy
Cuts at: 0 1031 1077
Size: 1031 46
EcoRI G'AATT_C
Cuts at: 0 849 1077
Size: 849 228
EcoRV GAT'ATC
Cuts at: 0 573 1077
Size: 573 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 1077
Size: 355 722
FspI TGC'GCA
Cuts at: 0 1038 1077
Size: 1038 39
HaeII r_GCGC'y
Cuts at: 0 1063 1077
Size: 1063 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1077
Size: 529 32 516
Fragments arranged by size:
529 516 32
KasI G'GCGC_C
Cuts at: 0 1059 1077
Size: 1059 18
MslI CAynn'nnrTG
Cuts at: 0 739 841 1077
Size: 739 102 236
Fragments arranged by size:
739 236 102
MspA1I CmG'CkG
Cuts at: 0 417 927 1077
Size: 417 510 150
Fragments arranged by size:
510 417 150
NarI GG'CG_CC
Cuts at: 0 1060 1077
Size: 1060 17
NspI r_CATG'y
Cuts at: 0 1043 1077
Size: 1043 34
PfoI T'CCnGG_A
Cuts at: 0 902 1077
Size: 902 175
PshAI GACnn'nnGTC
Cuts at: 0 911 1077
Size: 911 166
PstI C_TGCA'G
Cuts at: 0 324 741 1077
Size: 324 417 336
Fragments arranged by size:
417 336 324
PvuII CAG'CTG
Cuts at: 0 417 927 1077
Size: 417 510 150
Fragments arranged by size:
510 417 150
SfcI C'TryA_G
Cuts at: 0 320 528 737 830 861 1077
Size: 320 208 209 93 31 216
Fragments arranged by size:
320 216 209 208 93 31
SfoI GGC'GCC
Cuts at: 0 1061 1077
Size: 1061 16
SphI G_CATG'C
Cuts at: 0 1043 1077
Size: 1043 34
StuI AGG'CCT
Cuts at: 0 709 1077
Size: 709 368
StyI C'CwwG_G
Cuts at: 0 5 42 1026 1077
Size: 5 37 984 51
Fragments arranged by size:
984 51 37 5
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BclI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI
BsgI BsmI Bsp1286I BspMI BssHII BssSI BstXI BstZ17I
BtgI BtgZI BtsI DraIII EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I KasI
MslI MspA1I NarI NspI PfoI PshAI PstI PvuII
SfcI SfoI SphI StuI StyI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstYI Bsu36I ClaI CspCI DraI DrdI
EaeI EagI EcoICRI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI