(Linear) (Six-base) MAPSORT of: KIEE5073.seq  Check: 6807  from: 1  to: 387



KIEE5073.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     136     387 
   Size:        136     251
 
ApaLI G'TGCA_C

Cuts at:      0      84     387 
   Size:         84     303
 
BanI G'GyrC_C

Cuts at:      0     307     322     355     387 
   Size:        307      15      33      32

  Fragments arranged by size: 

                 307      33      32      15
 
BanII G_rGCy'C

Cuts at:      0     298     387 
   Size:        298      89
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     349     383     387 
   Size:        349      34       4

  Fragments arranged by size: 

                 349      34       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     178     387 
   Size:        178     209
 
BglI GCCn_nnn'nGGC

Cuts at:      0      98     387 
   Size:         98     289
 
Bme1580I G_kGCm'C

Cuts at:      0      88     325     387 
   Size:         88     237      62

  Fragments arranged by size: 

                 237      88      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     387 
   Size:        210     177
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     387 
   Size:        282     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     217     355     387 
   Size:        217     138      32

  Fragments arranged by size: 

                 217     138      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133     387 
   Size:        103      30     254

  Fragments arranged by size: 

                 254     103      30
 
BseYI C'CCAG_C

Cuts at:      0      50     163     213     387 
   Size:         50     113      50     174

  Fragments arranged by size: 

                 174     113      50      50
 
BsiHKAI G_wGCw'C

Cuts at:      0      88     298     387 
   Size:         88     210      89

  Fragments arranged by size: 

                 210      89      88
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     298     325     387 
   Size:         88     210      27      62

  Fragments arranged by size: 

                 210      88      62      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     387 
   Size:        136     251
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      76     387 
   Size:         76     311
 
BtgI C'CryG_G

Cuts at:      0      69     387 
   Size:         69     318
 
EcoICRI GAG'CTC

Cuts at:      0     296     387 
   Size:        296      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     387 
   Size:        210     177
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     387 
   Size:         91     296
 
HaeII r_GCGC'y

Cuts at:      0     237     387 
   Size:        237     150
 
MslI CAynn'nnrTG

Cuts at:      0      74     387 
   Size:         74     313
 
MspA1I CmG'CkG

Cuts at:      0     167     305     387 
   Size:        167     138      82

  Fragments arranged by size: 

                 167     138      82
 
NcoI C'CATG_G

Cuts at:      0      69     387 
   Size:         69     318
 
PfoI T'CCnGG_A

Cuts at:      0     121     387 
   Size:        121     266
 
SacI G_AGCT'C

Cuts at:      0     298     387 
   Size:        298      89
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      98     387 
   Size:         98     289
 
SmlI C'TyrA_G

Cuts at:      0     297     387 
   Size:        297      90
 
StyI C'CwwG_G

Cuts at:      0      69      95     227     387 
   Size:         69      26     132     160

  Fragments arranged by size: 

                 160     132      69      26
 

 Enzymes that do cut:

     AarI    ApaLI     BanI    BanII     BcgI    BciVI     BglI Bme1580I
     BpmI    BpuEI     BsaI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI
    BstXI     BtgI  EcoICRI  Eco57MI EcoO109I    HaeII     MslI   MspA1I
     NcoI     PfoI     SacI     SfiI     SmlI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI