(Linear) (Six-base) MAPSORT of: KIEE5070.seq  Check: 4573  from: 1  to: 279



KIEE5070.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

Acc65I G'GTAC_C

Cuts at:      0     143     279 
   Size:        143     136
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     279 
   Size:        236      43
 
ApaI G_GGCC'C

Cuts at:      0      35     279 
   Size:         35     244
 
ApoI r'AATT_y

Cuts at:      0     216     279 
   Size:        216      63
 
AvaI C'yCGr_G

Cuts at:      0      28     119     279 
   Size:         28      91     160

  Fragments arranged by size: 

                 160      91      28
 
BanI G'GyrC_C

Cuts at:      0     124     143     279 
   Size:        124      19     136

  Fragments arranged by size: 

                 136     124      19
 
BanII G_rGCy'C

Cuts at:      0      35     211     279 
   Size:         35     176      68

  Fragments arranged by size: 

                 176      68      35
 
BbeI G_GCGC'C

Cuts at:      0     128     279 
   Size:        128     151
 
BbvCI CC'TCA_GC

Cuts at:      0     148     279 
   Size:        148     131
 
Bme1580I G_kGCm'C

Cuts at:      0      35     197     279 
   Size:         35     162      82

  Fragments arranged by size: 

                 162      82      35
 
BmgBI CAC'GTC

Cuts at:      0      81     279 
   Size:         81     198
 
Bpu10I CC'TnA_GC

Cuts at:      0     148     279 
   Size:        148     131
 
BsaHI Gr'CG_yC

Cuts at:      0     125     279 
   Size:        125     154
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     118     190     193     279 
   Size:        115       3      72       3      86

  Fragments arranged by size: 

                 115      86      72       3       3
 
BseYI C'CCAG_C

Cuts at:      0     158     271     279 
   Size:        158     113       8

  Fragments arranged by size: 

                 158     113       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     279 
   Size:         67     212
 
BsiHKAI G_wGCw'C

Cuts at:      0     211     279 
   Size:        211      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     197     211     279 
   Size:         35     162      14      68

  Fragments arranged by size: 

                 162      68      35      14
 
BstYI r'GATC_y

Cuts at:      0      50     279 
   Size:         50     229
 
BtgI C'CryG_G

Cuts at:      0     189     279 
   Size:        189      90
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     127     279 
   Size:        127     152
 
EcoICRI GAG'CTC

Cuts at:      0     209     279 
   Size:        209      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     279 
   Size:        236      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     279 
   Size:        132     147
 
EcoRI G'AATT_C

Cuts at:      0     216     279 
   Size:        216      63
 
HaeII r_GCGC'y

Cuts at:      0     128     279 
   Size:        128     151
 
KasI G'GCGC_C

Cuts at:      0     124     279 
   Size:        124     155
 
KpnI G_GTAC'C

Cuts at:      0     147     279 
   Size:        147     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     279 
   Size:         71     208
 
MspA1I CmG'CkG

Cuts at:      0     191     279 
   Size:        191      88
 
NarI GG'CG_CC

Cuts at:      0     125     279 
   Size:        125     154
 
PfoI T'CCnGG_A

Cuts at:      0      65     279 
   Size:         65     214
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     279 
   Size:        132     147
 
PspOMI G'GGCC_C

Cuts at:      0      31     279 
   Size:         31     248
 
SacI G_AGCT'C

Cuts at:      0     211     279 
   Size:        211      68
 
SacII CC_GC'GG

Cuts at:      0     192     279 
   Size:        192      87
 
SanDI GG'GwC_CC

Cuts at:      0     132     279 
   Size:        132     147
 
SfoI GGC'GCC

Cuts at:      0     126     279 
   Size:        126     153
 
SmaI CCC'GGG

Cuts at:      0      30     279 
   Size:         30     249
 
SrfI GCCC'GGGC

Cuts at:      0      30     279 
   Size:         30     249
 
XmaI C'CCGG_G

Cuts at:      0      28     279 
   Size:         28     251
 

 Enzymes that do cut:

   Acc65I     AcuI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
    BbvCI Bme1580I    BmgBI   Bpu10I    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BstYI     BtgI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    HaeII     KasI     KpnI     MmeI   MspA1I     NarI     PfoI
    PpuMI   PspOMI     SacI    SacII    SanDI     SfoI     SmaI     SrfI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI     MscI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI