Restriction map for
pFN21AE5065
(Linear) (Six-base) MAPSORT of: KIEE5065.seq Check: 9694 from: 1 to: 1770
KIEE5065.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
Acc65I G'GTAC_C
Cuts at: 0 1076 1770
Size: 1076 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1770
Size: 1024 746
AflIII A'CryG_T
Cuts at: 0 401 1725 1770
Size: 401 1324 45
Fragments arranged by size:
1324 401 45
AhdI GACnn_n'nnGTC
Cuts at: 0 897 912 1770
Size: 897 15 858
Fragments arranged by size:
897 858 15
ApaI G_GGCC'C
Cuts at: 0 600 726 1770
Size: 600 126 1044
Fragments arranged by size:
1044 600 126
ApaLI G'TGCA_C
Cuts at: 0 817 1770
Size: 817 953
ApoI r'AATT_y
Cuts at: 0 1645 1770
Size: 1645 125
AvaI C'yCGr_G
Cuts at: 0 208 280 295 480 880 902 1770
Size: 208 72 15 185 400 22 868
Fragments arranged by size:
868 400 208 185 72 22 15
BamHI G'GATC_C
Cuts at: 0 101 1770
Size: 101 1669
BanI G'GyrC_C
Cuts at: 0 367 417 485 1076 1098 1392 1770
Size: 367 50 68 591 22 294 378
Fragments arranged by size:
591 378 367 294 68 50 22
BanII G_rGCy'C
Cuts at: 0 207 559 600 635 668 726 751 836
Size: 207 352 41 35 33 58 25 85
Cuts at: 836 1040 1751 1770
Size: 204 711 19
Fragments arranged by size:
711 352 207 204 85 58 41 35
33 25 19
BbeI G_GCGC'C
Cuts at: 0 371 489 1102 1770
Size: 371 118 613 668
Fragments arranged by size:
668 613 371 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 1133 1770
Size: 1133 637
BbvCI CC'TCA_GC
Cuts at: 0 496 1770
Size: 496 1274
BglI GCCn_nnn'nGGC
Cuts at: 0 137 1566 1770
Size: 137 1429 204
Fragments arranged by size:
1429 204 137
BglII A'GATC_T
Cuts at: 0 761 1770
Size: 761 1009
BlpI GC'TnA_GC
Cuts at: 0 553 1770
Size: 553 1217
Bme1580I G_kGCm'C
Cuts at: 0 422 600 726 821 1770
Size: 422 178 126 95 949
Fragments arranged by size:
949 422 178 126 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 1752 1770
Size: 1752 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1770
Size: 850 920
Bpu10I CC'TnA_GC
Cuts at: 0 496 1770
Size: 496 1274
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 1587 1770
Size: 277 1310 183
Fragments arranged by size:
1310 277 183
BsaAI yAC'GTr
Cuts at: 0 404 1728 1770
Size: 404 1324 42
Fragments arranged by size:
1324 404 42
BsaHI Gr'CG_yC
Cuts at: 0 368 459 486 1099 1315 1770
Size: 368 91 27 613 216 455
Fragments arranged by size:
613 455 368 216 91 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 782 812 1770
Size: 782 30 958
Fragments arranged by size:
958 782 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 661 705 727 1770
Size: 226 435 44 22 1043
Fragments arranged by size:
1043 435 226 44 22
BseYI C'CCAG_C
Cuts at: 0 237 422 582 609 845 1770
Size: 237 185 160 27 236 925
Fragments arranged by size:
925 237 236 185 160 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1287 1399 1770
Size: 99 1188 112 371
Fragments arranged by size:
1188 371 112 99
BsiEI CG_ry'CG
Cuts at: 0 517 913 971 1770
Size: 517 396 58 799
Fragments arranged by size:
799 517 396 58
BsiHKAI G_wGCw'C
Cuts at: 0 530 550 635 821 1034 1751 1770
Size: 530 20 85 186 213 717 19
Fragments arranged by size:
717 530 213 186 85 20 19
Bsp1286I G_dGCh'C
Cuts at: 0 207 422 530 550 559 600 635 668
Size: 207 215 108 20 9 41 35 33
Cuts at: 668 726 751 821 836 1034 1040 1751 1770
Size: 58 25 70 15 198 6 711 19
Fragments arranged by size:
711 215 207 198 108 70 58 41
35 33 25 20 19 15 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 726 989 1088 1770
Size: 33 693 263 99 682
Fragments arranged by size:
693 682 263 99 33
BsrBI CCG'CTC
Cuts at: 0 87 1243 1770
Size: 87 1156 527
Fragments arranged by size:
1156 527 87
BsrFI r'CCGG_y
Cuts at: 0 13 20 27 908 966 970 1095 1120
Size: 13 7 7 881 58 4 125 25
Cuts at: 1120 1317 1512 1770
Size: 197 195 258
Fragments arranged by size:
881 258 197 195 125 58 25 13
7 7 4
BssHII G'CGCG_C
Cuts at: 0 9 56 920 1770
Size: 9 47 864 850
Fragments arranged by size:
864 850 47 9
BssSI C'ACGA_G
Cuts at: 0 313 1033 1584 1770
Size: 313 720 551 186
Fragments arranged by size:
720 551 313 186
BstBI TT'CG_AA
Cuts at: 0 362 1770
Size: 362 1408
BstEII G'GTnAC_C
Cuts at: 0 1330 1770
Size: 1330 440
BstYI r'GATC_y
Cuts at: 0 101 761 803 995 1490 1770
Size: 101 660 42 192 495 280
Fragments arranged by size:
660 495 280 192 101 42
Bsu36I CC'TnA_GG
Cuts at: 0 1232 1770
Size: 1232 538
BtgI C'CryG_G
Cuts at: 0 195 264 668 742 1347 1466 1494 1770
Size: 195 69 404 74 605 119 28 276
Fragments arranged by size:
605 404 276 195 119 74 69 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1709 1744 1770
Size: 1709 35 26
Fragments arranged by size:
1709 35 26
EaeI y'GGCC_r
Cuts at: 0 198 514 677 910 968 1344 1463 1510
Size: 198 316 163 233 58 376 119 47
Cuts at: 1510 1539 1770
Size: 29 231
Fragments arranged by size:
376 316 233 231 198 163 119 58
47 29
EagI C'GGCC_G
Cuts at: 0 514 910 968 1770
Size: 514 396 58 802
Fragments arranged by size:
802 514 396 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 988 1147 1770
Size: 496 492 159 623
Fragments arranged by size:
623 496 492 159
EcoICRI GAG'CTC
Cuts at: 0 633 1749 1770
Size: 633 1116 21
Fragments arranged by size:
1116 633 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1024 1770
Size: 850 174 746
Fragments arranged by size:
850 746 174
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1285 1770
Size: 1285 485
EcoO109I rG'GnC_Cy
Cuts at: 0 596 722 723 738 852 876 1770
Size: 596 126 1 15 114 24 894
Fragments arranged by size:
894 596 126 114 24 15 1
HaeII r_GCGC'y
Cuts at: 0 371 489 1102 1363 1770
Size: 371 118 613 261 407
Fragments arranged by size:
613 407 371 261 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 782 814 883 898 915 930 1279 1311
Size: 782 32 69 15 17 15 349 32
Cuts at: 1311 1650 1682 1770
Size: 339 32 88
Fragments arranged by size:
782 349 339 88 69 32 32 32
17 15 15
HincII GTy'rAC
Cuts at: 0 51 186 1770
Size: 51 135 1584
Fragments arranged by size:
1584 135 51
KasI G'GCGC_C
Cuts at: 0 367 485 1098 1770
Size: 367 118 613 672
Fragments arranged by size:
672 613 367 118
KpnI G_GTAC'C
Cuts at: 0 1080 1770
Size: 1080 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1624 1770
Size: 1412 212 146
Fragments arranged by size:
1412 212 146
MscI TGG'CCA
Cuts at: 0 1346 1770
Size: 1346 424
MspA1I CmG'CkG
Cuts at: 0 159 237 266 324 387 617 744 1226
Size: 159 78 29 58 63 230 127 482
Cuts at: 1226 1770
Size: 544
Fragments arranged by size:
544 482 230 159 127 78 63 58
29
NaeI GCC'GGC
Cuts at: 0 15 22 968 972 1097 1319 1514 1770
Size: 15 7 946 4 125 222 195 256
Fragments arranged by size:
946 256 222 195 125 15 7 4
NarI GG'CG_CC
Cuts at: 0 368 486 1099 1770
Size: 368 118 613 671
Fragments arranged by size:
671 613 368 118
NcoI C'CATG_G
Cuts at: 0 195 1494 1770
Size: 195 1299 276
Fragments arranged by size:
1299 276 195
NgoMIV G'CCGG_C
Cuts at: 0 13 20 966 970 1095 1317 1512 1770
Size: 13 7 946 4 125 222 195 258
Fragments arranged by size:
946 258 222 195 125 13 7 4
NotI GC'GGCC_GC
Cuts at: 0 514 1770
Size: 514 1256
PasI CC'CwG_GG
Cuts at: 0 593 780 1185 1770
Size: 593 187 405 585
Fragments arranged by size:
593 585 405 187
PflMI CCAn_nnn'nTGG
Cuts at: 0 1017 1770
Size: 1017 753
PfoI T'CCnGG_A
Cuts at: 0 395 1770
Size: 395 1375
PmlI CAC'GTG
Cuts at: 0 1728 1770
Size: 1728 42
PpuMI rG'GwC_Cy
Cuts at: 0 738 876 1770
Size: 738 138 894
Fragments arranged by size:
894 738 138
PshAI GACnn'nnGTC
Cuts at: 0 1532 1770
Size: 1532 238
PspOMI G'GGCC_C
Cuts at: 0 596 722 1770
Size: 596 126 1048
Fragments arranged by size:
1048 596 126
PvuII CAG'CTG
Cuts at: 0 237 1226 1770
Size: 237 989 544
Fragments arranged by size:
989 544 237
SacI G_AGCT'C
Cuts at: 0 635 1751 1770
Size: 635 1116 19
Fragments arranged by size:
1116 635 19
SacII CC_GC'GG
Cuts at: 0 267 745 1770
Size: 267 478 1025
Fragments arranged by size:
1025 478 267
SfcI C'TryA_G
Cuts at: 0 768 1770
Size: 768 1002
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 137 1770
Size: 137 1633
SfoI GGC'GCC
Cuts at: 0 369 487 1100 1770
Size: 369 118 613 670
Fragments arranged by size:
670 613 369 118
SgrAI Cr'CCGG_yG
Cuts at: 0 1095 1770
Size: 1095 675
SmaI CCC'GGG
Cuts at: 0 482 904 1770
Size: 482 422 866
Fragments arranged by size:
866 482 422
StyI C'CwwG_G
Cuts at: 0 195 1494 1504 1770
Size: 195 1299 10 266
Fragments arranged by size:
1299 266 195 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 1770
Size: 405 1365
Tth111I GACn'n_nGTC
Cuts at: 0 1477 1605 1770
Size: 1477 128 165
Fragments arranged by size:
1477 165 128
XmaI C'CCGG_G
Cuts at: 0 480 902 1770
Size: 480 422 868
Fragments arranged by size:
868 480 422
Enzymes that do cut:
Acc65I AcuI AflIII AhdI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI Bsu36I
BtgI CspCI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PvuII SacI SacII
SfcI SfiI SfoI SgrAI SmaI StyI TaqII Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstAPI BstXI BstZ17I BtgZI BtsI ClaI DraI
DraIII DrdI EarI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI MfeI MluI MslI NdeI NheI
NruI NsiI NspI PacI PciI PmeI PpiI PsiI
PspXI PsrI PstI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmnI ZraI