(Linear) (Six-base) MAPSORT of: KIEE5063.seq  Check: 4145  from: 1  to: 1551



KIEE5063.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:58  ..

AccI GT'mk_AC

Cuts at:      0       5    1551 
   Size:          5    1546
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1325    1551 
   Size:       1325     226
 
AgeI A'CCGG_T

Cuts at:      0     509     593    1551 
   Size:        509      84     958

  Fragments arranged by size: 

                 958     509      84
 
ApoI r'AATT_y

Cuts at:      0     120     259     371     623     707     959    1551 
   Size:        120     139     112     252      84     252     592

  Fragments arranged by size: 

                 592     252     252     139     120     112      84
 
BbvCI CC'TCA_GC

Cuts at:      0    1104    1551 
   Size:       1104     447
 
BfrBI ATG'CAT

Cuts at:      0     157    1551 
   Size:        157    1394
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      10     809    1551 
   Size:         10     799     742

  Fragments arranged by size: 

                 799     742      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     485     569     905    1073    1409    1493    1551 
   Size:        485      84     336     168     336      84      58

  Fragments arranged by size: 

                 485     336     336     168      84      84      58
 
Bpu10I CC'TnA_GC

Cuts at:      0    1104    1551 
   Size:       1104     447
 
BsaAI yAC'GTr

Cuts at:      0    1372    1551 
   Size:       1372     179
 
BsaWI w'CCGG_w

Cuts at:      0     509     593    1551 
   Size:        509      84     958

  Fragments arranged by size: 

                 958     509      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1352    1551 
   Size:       1352     199
 
BsiHKAI G_wGCw'C

Cuts at:      0      59    1551 
   Size:         59    1492
 
Bsp1286I G_dGCh'C

Cuts at:      0      59    1551 
   Size:         59    1492
 
BsrDI GCAATG_nn'

Cuts at:      0    1344    1551 
   Size:       1344     207
 
BsrFI r'CCGG_y

Cuts at:      0     509     593    1551 
   Size:        509      84     958

  Fragments arranged by size: 

                 958     509      84
 
BsrGI T'GTAC_A

Cuts at:      0     167    1551 
   Size:        167    1384
 
BtgI C'CryG_G

Cuts at:      0     324    1551 
   Size:        324    1227
 
DraI TTT'AAA

Cuts at:      0    1011    1551 
   Size:       1011     540
 
EaeI y'GGCC_r

Cuts at:      0      49    1551 
   Size:         49    1502
 
EarI CTCTTCn'nnn_

Cuts at:      0     629    1551 
   Size:        629     922
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     485     569     905    1073    1325    1409    1493    1551 
   Size:        485      84     336     168     252      84      84      58

  Fragments arranged by size: 

                 485     336     252     168      84      84      84      58
            
 
EcoRI G'AATT_C

Cuts at:      0     623     707     959    1551 
   Size:        623      84     252     592

  Fragments arranged by size: 

                 623     592     252      84
 
HindIII A'AGCT_T

Cuts at:      0     500     584    1004    1551 
   Size:        500      84     420     547

  Fragments arranged by size: 

                 547     500     420      84
 
MscI TGG'CCA

Cuts at:      0      51    1551 
   Size:         51    1500
 
MslI CAynn'nnrTG

Cuts at:      0     146     816    1496    1551 
   Size:        146     670     680      55

  Fragments arranged by size: 

                 680     670     146      55
 
NdeI CA'TA_TG

Cuts at:      0      99     713    1133    1551 
   Size:         99     614     420     418

  Fragments arranged by size: 

                 614     420     418      99
 
NsiI A_TGCA'T

Cuts at:      0     159    1551 
   Size:        159    1392
 
NspI r_CATG'y

Cuts at:      0     161     345    1551 
   Size:        161     184    1206

  Fragments arranged by size: 

                1206     184     161
 
PsiI TTA'TAA

Cuts at:      0     182    1551 
   Size:        182    1369
 
SphI G_CATG'C

Cuts at:      0     345    1551 
   Size:        345    1206
 
StuI AGG'CCT

Cuts at:      0     670    1090    1551 
   Size:        670     420     461

  Fragments arranged by size: 

                 670     461     420
 
TatI w'GTAC_w

Cuts at:      0     167    1551 
   Size:        167    1384
 
XmnI GAAnn'nnTTC

Cuts at:      0     307    1551 
   Size:        307    1244
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     ApoI    BbvCI    BfrBI     BmrI     BpmI
   Bpu10I    BsaAI    BsaWI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrFI
    BsrGI     BtgI     DraI     EaeI     EarI  Eco57MI    EcoRI  HindIII
     MscI     MslI     NdeI     NsiI     NspI     PsiI     SphI     StuI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI