(Linear) (Six-base) MAPSORT of: KIEE5060.seq  Check: 3511  from: 1  to: 1179



KIEE5060.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     866    1179 
   Size:        866     313
 
Acc65I G'GTAC_C

Cuts at:      0     104     452    1179 
   Size:        104     348     727

  Fragments arranged by size: 

                 727     348     104
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    1179 
   Size:        458     721
 
AflII C'TTAA_G

Cuts at:      0     183     525     843    1179 
   Size:        183     342     318     336

  Fragments arranged by size: 

                 342     336     318     183
 
AflIII A'CryG_T

Cuts at:      0     385    1179 
   Size:        385     794
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     232    1179 
   Size:        232     947
 
ApoI r'AATT_y

Cuts at:      0     120     312     496     544    1012    1179 
   Size:        120     192     184      48     468     167

  Fragments arranged by size: 

                 468     192     184     167     120      48
 
AseI AT'TA_AT

Cuts at:      0     236     397    1179 
   Size:        236     161     782

  Fragments arranged by size: 

                 782     236     161
 
AvaI C'yCGr_G

Cuts at:      0     302    1179 
   Size:        302     877
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      88     121    1179 
   Size:         88      33    1058

  Fragments arranged by size: 

                1058      88      33
 
BanI G'GyrC_C

Cuts at:      0     104     452    1179 
   Size:        104     348     727

  Fragments arranged by size: 

                 727     348     104
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     942    1179 
   Size:        942     237
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     655    1055    1179 
   Size:        655     400     124

  Fragments arranged by size: 

                 655     400     124
 
BclI T'GATC_A

Cuts at:      0     735    1179 
   Size:        735     444
 
BfrBI ATG'CAT

Cuts at:      0     209    1179 
   Size:        209     970
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     372     374    1179 
   Size:        372       2     805

  Fragments arranged by size: 

                 805     372       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1179 
   Size:        665     514
 
BsaBI GATnn'nnATC

Cuts at:      0     475     536     679    1179 
   Size:        475      61     143     500

  Fragments arranged by size: 

                 500     475     143      61
 
BsaWI w'CCGG_w

Cuts at:      0     686    1179 
   Size:        686     493
 
BseYI C'CCAG_C

Cuts at:      0     880    1179 
   Size:        880     299
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42    1179 
   Size:         42    1137
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      58    1179 
   Size:         58    1121
 
BspEI T'CCGG_A

Cuts at:      0     686    1179 
   Size:        686     493
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     866    1154    1179 
   Size:        866     288      25

  Fragments arranged by size: 

                 866     288      25
 
BsrDI GCAATG_nn'

Cuts at:      0    1094    1179 
   Size:       1094      85
 
BstYI r'GATC_y

Cuts at:      0     306    1179 
   Size:        306     873
 
BtsI GCAGTG_nn'

Cuts at:      0    1125    1139    1179 
   Size:       1125      14      40

  Fragments arranged by size: 

                1125      40      14
 
DraI TTT'AAA

Cuts at:      0     801    1179 
   Size:        801     378
 
EaeI y'GGCC_r

Cuts at:      0     425    1179 
   Size:        425     754
 
EarI CTCTTCn'nnn_

Cuts at:      0    1070    1179 
   Size:       1070     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     882    1179 
   Size:        882     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     665    1179 
   Size:        458     207     514

  Fragments arranged by size: 

                 514     458     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0     924    1179 
   Size:        924     255
 
EcoRI G'AATT_C

Cuts at:      0     496    1179 
   Size:        496     683
 
HincII GTy'rAC

Cuts at:      0      36    1179 
   Size:         36    1143
 
HindIII A'AGCT_T

Cuts at:      0     777     840    1179 
   Size:        777      63     339

  Fragments arranged by size: 

                 777     339      63
 
KpnI G_GTAC'C

Cuts at:      0     108     456    1179 
   Size:        108     348     723

  Fragments arranged by size: 

                 723     348     108
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     639     984    1179 
   Size:        204     435     345     195

  Fragments arranged by size: 

                 435     345     204     195
 
MscI TGG'CCA

Cuts at:      0     427    1179 
   Size:        427     752
 
MspA1I CmG'CkG

Cuts at:      0     102     643    1179 
   Size:        102     541     536

  Fragments arranged by size: 

                 541     536     102
 
NsiI A_TGCA'T

Cuts at:      0     211    1179 
   Size:        211     968
 
NspI r_CATG'y

Cuts at:      0     745    1179 
   Size:        745     434
 
PasI CC'CwG_GG

Cuts at:      0      59    1179 
   Size:         59    1120
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     643     834     940    1179 
   Size:        643     191     106     239

  Fragments arranged by size: 

                 643     239     191     106
 
PsiI TTA'TAA

Cuts at:      0     749    1179 
   Size:        749     430
 
PstI C_TGCA'G

Cuts at:      0    1031    1134    1179 
   Size:       1031     103      45

  Fragments arranged by size: 

                1031     103      45
 
PvuII CAG'CTG

Cuts at:      0     643    1179 
   Size:        643     536
 
SfcI C'TryA_G

Cuts at:      0      16    1027    1130    1179 
   Size:         16    1011     103      49

  Fragments arranged by size: 

                1011     103      49      16
 
SmlI C'TyrA_G

Cuts at:      0     183     525     843    1179 
   Size:        183     342     318     336

  Fragments arranged by size: 

                 342     336     318     183
 
StyI C'CwwG_G

Cuts at:      0     862    1179 
   Size:        862     317
 
XmnI GAAnn'nnTTC

Cuts at:      0      53    1179 
   Size:         53    1126
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII   AflIII     AlfI     ApoI     AseI
     AvaI     BaeI     BanI     BbsI    BciVI     BclI    BfrBI     BplI
     BpmI    BsaBI    BsaWI    BseYI     BsgI    BsmBI    BspEI    BspMI
    BsrDI    BstYI     BtsI     DraI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI   HincII  HindIII     KpnI     MmeI     MscI   MspA1I
     NsiI     NspI     PasI    PflMI     PsiI     PstI    PvuII     SfcI
     SmlI     StyI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI    BanII
     BbeI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I     HpaI     KasI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI