Restriction map for
pFN21AE5057
(Linear) (Six-base) MAPSORT of: KIEE5057.seq Check: 8890 from: 1 to: 576
KIEE5057.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 576
Size: 207 369
AflIII A'CryG_T
Cuts at: 0 94 576
Size: 94 482
AleI CACnn'nnGTG
Cuts at: 0 97 576
Size: 97 479
ApoI r'AATT_y
Cuts at: 0 499 576
Size: 499 77
AvaI C'yCGr_G
Cuts at: 0 250 576
Size: 250 326
BanI G'GyrC_C
Cuts at: 0 289 576
Size: 289 287
BanII G_rGCy'C
Cuts at: 0 179 431 576
Size: 179 252 145
Fragments arranged by size:
252 179 145
BbeI G_GCGC'C
Cuts at: 0 293 576
Size: 293 283
BbvCI CC'TCA_GC
Cuts at: 0 158 576
Size: 158 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 521 576
Size: 487 34 55
Fragments arranged by size:
487 55 34
BglI GCCn_nnn'nGGC
Cuts at: 0 304 576
Size: 304 272
Bme1580I G_kGCm'C
Cuts at: 0 146 251 576
Size: 146 105 325
Fragments arranged by size:
325 146 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 336 576
Size: 336 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 576
Size: 343 233
Bpu10I CC'TnA_GC
Cuts at: 0 158 576
Size: 158 418
BsaI GGTCTCn'nnnn_
Cuts at: 0 132 221 576
Size: 132 89 355
Fragments arranged by size:
355 132 89
BsaAI yAC'GTr
Cuts at: 0 95 576
Size: 95 481
BsaHI Gr'CG_yC
Cuts at: 0 290 576
Size: 290 286
BsaWI w'CCGG_w
Cuts at: 0 77 576
Size: 77 499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 408 439 508 576
Size: 385 23 31 69 68
Fragments arranged by size:
385 69 68 31 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 174 576
Size: 37 137 402
Fragments arranged by size:
402 137 37
Bsp1286I G_dGCh'C
Cuts at: 0 146 179 251 431 576
Size: 146 33 72 180 145
Fragments arranged by size:
180 146 145 72 33
BspEI T'CCGG_A
Cuts at: 0 77 576
Size: 77 499
BsrFI r'CCGG_y
Cuts at: 0 53 576
Size: 53 523
Bsu36I CC'TnA_GG
Cuts at: 0 451 576
Size: 451 125
BtgI C'CryG_G
Cuts at: 0 260 299 576
Size: 260 39 277
Fragments arranged by size:
277 260 39
BtsI GCAGTG_nn'
Cuts at: 0 503 576
Size: 503 73
EaeI y'GGCC_r
Cuts at: 0 257 296 305 576
Size: 257 39 9 271
Fragments arranged by size:
271 257 39 9
EarI CTCTTCn'nnn_
Cuts at: 0 175 526 576
Size: 175 351 50
Fragments arranged by size:
351 175 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 343 576
Size: 207 136 233
Fragments arranged by size:
233 207 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 235 576
Size: 157 78 341
Fragments arranged by size:
341 157 78
EcoRI G'AATT_C
Cuts at: 0 499 576
Size: 499 77
HaeII r_GCGC'y
Cuts at: 0 293 576
Size: 293 283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 576
Size: 346 32 198
Fragments arranged by size:
346 198 32
KasI G'GCGC_C
Cuts at: 0 289 576
Size: 289 287
MslI CAynn'nnrTG
Cuts at: 0 97 484 576
Size: 97 387 92
Fragments arranged by size:
387 97 92
MspA1I CmG'CkG
Cuts at: 0 539 576
Size: 539 37
NarI GG'CG_CC
Cuts at: 0 290 576
Size: 290 286
PasI CC'CwG_GG
Cuts at: 0 569 576
Size: 569 7
PmlI CAC'GTG
Cuts at: 0 95 576
Size: 95 481
PshAI GACnn'nnGTC
Cuts at: 0 7 576
Size: 7 569
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 304 576
Size: 304 272
SfoI GGC'GCC
Cuts at: 0 291 576
Size: 291 285
SmaI CCC'GGG
Cuts at: 0 252 576
Size: 252 324
SrfI GCCC'GGGC
Cuts at: 0 252 576
Size: 252 324
StuI AGG'CCT
Cuts at: 0 30 576
Size: 30 546
StyI C'CwwG_G
Cuts at: 0 293 576
Size: 293 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 576
Size: 114 462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 396 576
Size: 318 78 180
Fragments arranged by size:
318 180 78
XmaI C'CCGG_G
Cuts at: 0 250 576
Size: 250 326
Enzymes that do cut:
AcuI AflIII AleI ApoI AvaI BanI BanII BbeI
BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BseRI BsgI Bsp1286I BspEI BsrFI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoRI
HaeII Hin4I KasI MslI MspA1I NarI PasI PmlI
PshAI SfiI SfoI SmaI SrfI StuI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaBI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI