(Linear) (Six-base) MAPSORT of: KIEE5054.seq  Check: 3819  from: 1  to: 459



KIEE5054.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:58  ..

AccI GT'mk_AC

Cuts at:      0     230     459 
   Size:        230     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     172     357     459 
   Size:         46     126     185     102

  Fragments arranged by size: 

                 185     126     102      46
 
AflIII A'CryG_T

Cuts at:      0     334     459 
   Size:        334     125
 
ApaLI G'TGCA_C

Cuts at:      0      12     459 
   Size:         12     447
 
ApoI r'AATT_y

Cuts at:      0      82     410     459 
   Size:         82     328      49

  Fragments arranged by size: 

                 328      82      49
 
BanI G'GyrC_C

Cuts at:      0     316     459 
   Size:        316     143
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     160     459 
   Size:        160     299
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     109     143     459 
   Size:        109      34     316

  Fragments arranged by size: 

                 316     109      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     139     459 
   Size:        139     320
 
Bme1580I G_kGCm'C

Cuts at:      0      16     319     459 
   Size:         16     303     140

  Fragments arranged by size: 

                 303     140      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     338     459 
   Size:        338     121
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     459 
   Size:         40     419
 
BseYI C'CCAG_C

Cuts at:      0       5     459 
   Size:          5     454
 
BsiHKAI G_wGCw'C

Cuts at:      0      16     459 
   Size:         16     443
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     319     459 
   Size:         16     303     140

  Fragments arranged by size: 

                 303     140      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     172     357     459 
   Size:         46     126     185     102

  Fragments arranged by size: 

                 185     126     102      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177     459 
   Size:        177     282
 
HincII GTy'rAC

Cuts at:      0     167     459 
   Size:        167     292
 
HpaI GTT'AAC

Cuts at:      0     167     459 
   Size:        167     292
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     459 
   Size:         83     376
 
MspA1I CmG'CkG

Cuts at:      0       9     459 
   Size:          9     450
 
NspI r_CATG'y

Cuts at:      0     338     459 
   Size:        338     121
 
PasI CC'CwG_GG

Cuts at:      0      72     459 
   Size:         72     387
 
PciI A'CATG_T

Cuts at:      0     334     459 
   Size:        334     125
 
PvuII CAG'CTG

Cuts at:      0       9     459 
   Size:          9     450
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    ApaLI     ApoI     BanI     BbsI     BcgI
     BglI Bme1580I     BsaI    BseRI    BseYI  BsiHKAI Bsp1286I  Eco57MI
     FalI   HincII     HpaI     MmeI   MspA1I     NspI     PasI     PciI
    PvuII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI