Restriction map for
pFN21AE5022
(Linear) (Six-base) MAPSORT of: KIEE5022.seq Check: 4132 from: 1 to: 1308
KIEE5022.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AatII G_ACGT'C
Cuts at: 0 439 1308
Size: 439 869
AccI GT'mk_AC
Cuts at: 0 1031 1308
Size: 1031 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1308
Size: 582 726
AflIII A'CryG_T
Cuts at: 0 387 1308
Size: 387 921
AgeI A'CCGG_T
Cuts at: 0 117 1074 1308
Size: 117 957 234
Fragments arranged by size:
957 234 117
AleI CACnn'nnGTG
Cuts at: 0 241 695 1308
Size: 241 454 613
Fragments arranged by size:
613 454 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 527 1308
Size: 527 781
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1308
Size: 708 600
ApaI G_GGCC'C
Cuts at: 0 18 86 1308
Size: 18 68 1222
Fragments arranged by size:
1222 68 18
AvaI C'yCGr_G
Cuts at: 0 85 907 1268 1308
Size: 85 822 361 40
Fragments arranged by size:
822 361 85 40
BanI G'GyrC_C
Cuts at: 0 34 108 114 140 784 1308
Size: 34 74 6 26 644 524
Fragments arranged by size:
644 524 74 34 26 6
BanII G_rGCy'C
Cuts at: 0 18 86 1308
Size: 18 68 1222
Fragments arranged by size:
1222 68 18
BbeI G_GCGC'C
Cuts at: 0 38 112 144 1308
Size: 38 74 32 1164
Fragments arranged by size:
1164 74 38 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 1308
Size: 588 720
BbvCI CC'TCA_GC
Cuts at: 0 28 1308
Size: 28 1280
BglI GCCn_nnn'nGGC
Cuts at: 0 32 62 487 562 616 874 1308
Size: 32 30 425 75 54 258 434
Fragments arranged by size:
434 425 258 75 54 32 30
Bme1580I G_kGCm'C
Cuts at: 0 18 86 173 478 702 1308
Size: 18 68 87 305 224 606
Fragments arranged by size:
606 305 224 87 68 18
BmgBI CAC'GTC
Cuts at: 0 249 865 1308
Size: 249 616 443
Fragments arranged by size:
616 443 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 1014 1308
Size: 1014 294
BmtI G_CTAG'C
Cuts at: 0 1265 1308
Size: 1265 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 1308
Size: 852 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1308
Size: 445 863
Bpu10I CC'TnA_GC
Cuts at: 0 28 220 1088 1308
Size: 28 192 868 220
Fragments arranged by size:
868 220 192 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1308
Size: 1114 194
BsaAI yAC'GTr
Cuts at: 0 390 693 1308
Size: 390 303 615
Fragments arranged by size:
615 390 303
BsaHI Gr'CG_yC
Cuts at: 0 35 109 141 436 530 1308
Size: 35 74 32 295 94 778
Fragments arranged by size:
778 295 94 74 35 32
BsaWI w'CCGG_w
Cuts at: 0 117 1074 1308
Size: 117 957 234
Fragments arranged by size:
957 234 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1287 1308
Size: 1287 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 484 1308
Size: 88 396 824
Fragments arranged by size:
824 396 88
BseYI C'CCAG_C
Cuts at: 0 701 1308
Size: 701 607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 325 1308
Size: 142 183 983
Fragments arranged by size:
983 183 142
BsiEI CG_ry'CG
Cuts at: 0 150 482 792 1308
Size: 150 332 310 516
Fragments arranged by size:
516 332 310 150
BsiHKAI G_wGCw'C
Cuts at: 0 527 1308
Size: 527 781
BsmBI CGTCTCn'nnnn_
Cuts at: 0 421 580 1308
Size: 421 159 728
Fragments arranged by size:
728 421 159
Bsp1286I G_dGCh'C
Cuts at: 0 18 86 173 478 527 702 1308
Size: 18 68 87 305 49 175 606
Fragments arranged by size:
606 305 175 87 68 49 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 1308
Size: 671 637
BsrDI GCAATG_nn'
Cuts at: 0 329 509 1308
Size: 329 180 799
Fragments arranged by size:
799 329 180
BsrFI r'CCGG_y
Cuts at: 0 111 117 137 514 1074 1308
Size: 111 6 20 377 560 234
Fragments arranged by size:
560 377 234 111 20 6
BssHII G'CGCG_C
Cuts at: 0 209 211 1308
Size: 209 2 1097
Fragments arranged by size:
1097 209 2
BssSI C'ACGA_G
Cuts at: 0 802 1308
Size: 802 506
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 402 1308
Size: 243 159 906
Fragments arranged by size:
906 243 159
BtgI C'CryG_G
Cuts at: 0 131 144 494 1308
Size: 131 13 350 814
Fragments arranged by size:
814 350 131 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 80 1033 1308
Size: 80 953 275
Fragments arranged by size:
953 275 80
BtsI GCAGTG_nn'
Cuts at: 0 171 511 1089 1308
Size: 171 340 578 219
Fragments arranged by size:
578 340 219 171
DraIII CAC_nnn'GTG
Cuts at: 0 944 1308
Size: 944 364
DrdI GACnn_nn'nnGTC
Cuts at: 0 435 535 1308
Size: 435 100 773
Fragments arranged by size:
773 435 100
EaeI y'GGCC_r
Cuts at: 0 8 201 353 392 404 479 488 554
Size: 8 193 152 39 12 75 9 66
Cuts at: 554 563 875 1308
Size: 9 312 433
Fragments arranged by size:
433 312 193 152 75 66 39 12
9 9 8
EagI C'GGCC_G
Cuts at: 0 479 1308
Size: 479 829
EarI CTCTTCn'nnn_
Cuts at: 0 65 280 313 421 1009 1308
Size: 65 215 33 108 588 299
Fragments arranged by size:
588 299 215 108 65 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 582 1308
Size: 445 137 726
Fragments arranged by size:
726 445 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 1308
Size: 682 626
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 1062 1308
Size: 14 1 1047 246
Fragments arranged by size:
1047 246 14 1
FspI TGC'GCA
Cuts at: 0 457 1308
Size: 457 851
HaeII r_GCGC'y
Cuts at: 0 38 112 144 261 622 1294 1308
Size: 38 74 32 117 361 672 14
Fragments arranged by size:
672 361 117 74 38 32 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 1308
Size: 1287 21
HincII GTy'rAC
Cuts at: 0 1032 1308
Size: 1032 276
KasI G'GCGC_C
Cuts at: 0 34 108 140 1308
Size: 34 74 32 1168
Fragments arranged by size:
1168 74 34 32
MscI TGG'CCA
Cuts at: 0 10 203 394 556 565 877 1308
Size: 10 193 191 162 9 312 431
Fragments arranged by size:
431 312 193 191 162 10 9
MslI CAynn'nnrTG
Cuts at: 0 241 695 758 931 1308
Size: 241 454 63 173 377
Fragments arranged by size:
454 377 241 173 63
MspA1I CmG'CkG
Cuts at: 0 32 146 484 705 747 1308
Size: 32 114 338 221 42 561
Fragments arranged by size:
561 338 221 114 42 32
NaeI GCC'GGC
Cuts at: 0 113 139 516 1308
Size: 113 26 377 792
Fragments arranged by size:
792 377 113 26
NarI GG'CG_CC
Cuts at: 0 35 109 141 1308
Size: 35 74 32 1167
Fragments arranged by size:
1167 74 35 32
NgoMIV G'CCGG_C
Cuts at: 0 111 137 514 1308
Size: 111 26 377 794
Fragments arranged by size:
794 377 111 26
NheI G'CTAG_C
Cuts at: 0 1261 1308
Size: 1261 47
NspI r_CATG'y
Cuts at: 0 242 1308
Size: 242 1066
PasI CC'CwG_GG
Cuts at: 0 1066 1308
Size: 1066 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1308
Size: 762 546
PmlI CAC'GTG
Cuts at: 0 390 693 1308
Size: 390 303 615
Fragments arranged by size:
615 390 303
PpuMI rG'GwC_Cy
Cuts at: 0 1062 1308
Size: 1062 246
PshAI GACnn'nnGTC
Cuts at: 0 592 1075 1308
Size: 592 483 233
Fragments arranged by size:
592 483 233
PspOMI G'GGCC_C
Cuts at: 0 14 82 1308
Size: 14 68 1226
Fragments arranged by size:
1226 68 14
PstI C_TGCA'G
Cuts at: 0 506 713 1084 1308
Size: 506 207 371 224
Fragments arranged by size:
506 371 224 207
PvuI CG_AT'CG
Cuts at: 0 792 1308
Size: 792 516
PvuII CAG'CTG
Cuts at: 0 705 1308
Size: 705 603
SacII CC_GC'GG
Cuts at: 0 147 1308
Size: 147 1161
SalI G'TCGA_C
Cuts at: 0 1030 1308
Size: 1030 278
SapI GCTCTTCn'nnn_
Cuts at: 0 280 313 1308
Size: 280 33 995
Fragments arranged by size:
995 280 33
SexAI A'CCwGG_T
Cuts at: 0 677 1308
Size: 677 631
SfcI C'TryA_G
Cuts at: 0 502 709 1080 1308
Size: 502 207 371 228
Fragments arranged by size:
502 371 228 207
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 487 562 1308
Size: 487 75 746
Fragments arranged by size:
746 487 75
SfoI GGC'GCC
Cuts at: 0 36 110 142 1308
Size: 36 74 32 1166
Fragments arranged by size:
1166 74 36 32
SgrAI Cr'CCGG_yG
Cuts at: 0 117 1308
Size: 117 1191
SmaI CCC'GGG
Cuts at: 0 87 1308
Size: 87 1221
SmlI C'TyrA_G
Cuts at: 0 907 1129 1308
Size: 907 222 179
Fragments arranged by size:
907 222 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 532 1308
Size: 187 345 776
Fragments arranged by size:
776 345 187
TatI w'GTAC_w
Cuts at: 0 737 1308
Size: 737 571
XhoI C'TCGA_G
Cuts at: 0 907 1308
Size: 907 401
XmaI C'CCGG_G
Cuts at: 0 85 1308
Size: 85 1223
ZraI GAC'GTC
Cuts at: 0 437 1308
Size: 437 871
Enzymes that do cut:
AatII AccI AcuI AflIII AgeI AleI AlfI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI
BstXI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
KasI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI
PvuI PvuII SacII SalI SapI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI TaqII TatI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI BsaI BsaBI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI EciI EcoICRI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NotI NruI NsiI
PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI
RsrII SacI SanDI SbfI ScaI SgfI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI Tth111I
XbaI XcmI XmnI