Restriction map for
pFN21AE5020
(Linear) (Six-base) MAPSORT of: KIEE5020.seq Check: 6412 from: 1 to: 1122
KIEE5020.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 907 1122
Size: 907 215
AatII G_ACGT'C
Cuts at: 0 869 1122
Size: 869 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 794 936 1122
Size: 439 355 142 186
Fragments arranged by size:
439 355 186 142
AflIII A'CryG_T
Cuts at: 0 458 1122
Size: 458 664
AhdI GACnn_n'nnGTC
Cuts at: 0 879 1122
Size: 879 243
AleI CACnn'nnGTG
Cuts at: 0 403 457 1122
Size: 403 54 665
Fragments arranged by size:
665 403 54
AlwNI CAG_nnn'CTG
Cuts at: 0 381 1098 1122
Size: 381 717 24
Fragments arranged by size:
717 381 24
ApoI r'AATT_y
Cuts at: 0 235 1122
Size: 235 887
AvaI C'yCGr_G
Cuts at: 0 85 1122
Size: 85 1037
BanI G'GyrC_C
Cuts at: 0 64 304 634 936 1122
Size: 64 240 330 302 186
Fragments arranged by size:
330 302 240 186 64
BbeI G_GCGC'C
Cuts at: 0 68 638 1122
Size: 68 570 484
Fragments arranged by size:
570 484 68
BglI GCCn_nnn'nGGC
Cuts at: 0 846 1122
Size: 846 276
BglII A'GATC_T
Cuts at: 0 1007 1122
Size: 1007 115
Bme1580I G_kGCm'C
Cuts at: 0 293 1122
Size: 293 829
BmgBI CAC'GTC
Cuts at: 0 459 1122
Size: 459 663
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1122
Size: 531 591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1122
Size: 1026 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1122
Size: 205 917
BsaAI yAC'GTr
Cuts at: 0 615 660 1122
Size: 615 45 462
Fragments arranged by size:
615 462 45
BsaBI GATnn'nnATC
Cuts at: 0 139 1122
Size: 139 983
BsaHI Gr'CG_yC
Cuts at: 0 65 80 224 635 866 1122
Size: 65 15 144 411 231 256
Fragments arranged by size:
411 256 231 144 65 15
BsaWI w'CCGG_w
Cuts at: 0 137 1122
Size: 137 985
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 967 997 1122
Size: 967 30 125
Fragments arranged by size:
967 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 127 145 559 844 1018 1122
Size: 124 3 18 414 285 174 104
Fragments arranged by size:
414 285 174 124 104 18 3
BseYI C'CCAG_C
Cuts at: 0 345 841 1122
Size: 345 496 281
Fragments arranged by size:
496 345 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 281 879 1122
Size: 24 257 598 243
Fragments arranged by size:
598 257 243 24
BsiEI CG_ry'CG
Cuts at: 0 527 927 1122
Size: 527 400 195
Fragments arranged by size:
527 400 195
BsiHKAI G_wGCw'C
Cuts at: 0 892 1122
Size: 892 230
Bsp1286I G_dGCh'C
Cuts at: 0 293 892 1122
Size: 293 599 230
Fragments arranged by size:
599 293 230
BspEI T'CCGG_A
Cuts at: 0 137 1122
Size: 137 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 422 884 907 1122
Size: 62 360 462 23 215
Fragments arranged by size:
462 360 215 62 23
BsrBI CCG'CTC
Cuts at: 0 654 762 908 929 1122
Size: 654 108 146 21 193
Fragments arranged by size:
654 193 146 108 21
BsrFI r'CCGG_y
Cuts at: 0 176 220 241 845 1122
Size: 176 44 21 604 277
Fragments arranged by size:
604 277 176 44 21
BssSI C'ACGA_G
Cuts at: 0 97 1122
Size: 97 1025
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 1122
Size: 15 1107
BstYI r'GATC_y
Cuts at: 0 134 1007 1122
Size: 134 873 115
Fragments arranged by size:
873 134 115
Bsu36I CC'TnA_GG
Cuts at: 0 730 827 1122
Size: 730 97 295
Fragments arranged by size:
730 295 97
BtgI C'CryG_G
Cuts at: 0 835 1122
Size: 835 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1028 1122
Size: 1028 94
EaeI y'GGCC_r
Cuts at: 0 524 924 1122
Size: 524 400 198
Fragments arranged by size:
524 400 198
EagI C'GGCC_G
Cuts at: 0 524 924 1122
Size: 524 400 198
Fragments arranged by size:
524 400 198
EarI CTCTTCn'nnn_
Cuts at: 0 770 1042 1122
Size: 770 272 80
Fragments arranged by size:
770 272 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 877 1122
Size: 877 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 439 794 936 1122
Size: 205 234 355 142 186
Fragments arranged by size:
355 234 205 186 142
EcoO109I rG'GnC_Cy
Cuts at: 0 707 1122
Size: 707 415
FspI TGC'GCA
Cuts at: 0 448 1122
Size: 448 674
HaeII r_GCGC'y
Cuts at: 0 68 638 959 1122
Size: 68 570 321 163
Fragments arranged by size:
570 321 163 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 769 801 865 897 1122
Size: 769 32 64 32 225
Fragments arranged by size:
769 225 64 32 32
HincII GTy'rAC
Cuts at: 0 590 1122
Size: 590 532
HindIII A'AGCT_T
Cuts at: 0 961 1122
Size: 961 161
KasI G'GCGC_C
Cuts at: 0 64 634 1122
Size: 64 570 488
Fragments arranged by size:
570 488 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 185 1122
Size: 71 114 937
Fragments arranged by size:
937 114 71
MslI CAynn'nnrTG
Cuts at: 0 403 457 1122
Size: 403 54 665
Fragments arranged by size:
665 403 54
MspA1I CmG'CkG
Cuts at: 0 183 357 837 1122
Size: 183 174 480 285
Fragments arranged by size:
480 285 183 174
NaeI GCC'GGC
Cuts at: 0 222 243 847 1122
Size: 222 21 604 275
Fragments arranged by size:
604 275 222 21
NarI GG'CG_CC
Cuts at: 0 65 635 1122
Size: 65 570 487
Fragments arranged by size:
570 487 65
NgoMIV G'CCGG_C
Cuts at: 0 220 241 845 1122
Size: 220 21 604 277
Fragments arranged by size:
604 277 220 21
NotI GC'GGCC_GC
Cuts at: 0 524 924 1122
Size: 524 400 198
Fragments arranged by size:
524 400 198
NspI r_CATG'y
Cuts at: 0 333 447 1122
Size: 333 114 675
Fragments arranged by size:
675 333 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1122
Size: 381 741
PfoI T'CCnGG_A
Cuts at: 0 869 1122
Size: 869 253
PshAI GACnn'nnGTC
Cuts at: 0 364 1122
Size: 364 758
PspXI vC'TCGA_Gb
Cuts at: 0 85 1122
Size: 85 1037
PstI C_TGCA'G
Cuts at: 0 203 626 644 881 1122
Size: 203 423 18 237 241
Fragments arranged by size:
423 241 237 203 18
PvuII CAG'CTG
Cuts at: 0 183 1122
Size: 183 939
SacII CC_GC'GG
Cuts at: 0 838 1122
Size: 838 284
SexAI A'CCwGG_T
Cuts at: 0 719 1122
Size: 719 403
SfcI C'TryA_G
Cuts at: 0 199 622 640 877 1122
Size: 199 423 18 237 245
Fragments arranged by size:
423 245 237 199 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 846 1122
Size: 846 276
SfoI GGC'GCC
Cuts at: 0 66 636 1122
Size: 66 570 486
Fragments arranged by size:
570 486 66
SgrAI Cr'CCGG_yG
Cuts at: 0 220 1122
Size: 220 902
SmlI C'TyrA_G
Cuts at: 0 85 1122
Size: 85 1037
SphI G_CATG'C
Cuts at: 0 447 1122
Size: 447 675
StuI AGG'CCT
Cuts at: 0 322 1122
Size: 322 800
StyI C'CwwG_G
Cuts at: 0 629 1122
Size: 629 493
TatI w'GTAC_w
Cuts at: 0 152 1122
Size: 152 970
Tth111I GACn'n_nGTC
Cuts at: 0 24 1122
Size: 24 1098
XhoI C'TCGA_G
Cuts at: 0 85 1122
Size: 85 1037
XmnI GAAnn'nnTTC
Cuts at: 0 400 949 1122
Size: 400 549 173
Fragments arranged by size:
549 400 173
ZraI GAC'GTC
Cuts at: 0 867 1122
Size: 867 255
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlwNI ApoI
AvaI BanI BbeI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI EaeI
EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I
HincII HindIII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PflMI PfoI PshAI PspXI PstI
PvuII SacII SexAI SfcI SfiI SfoI SgrAI SmlI
SphI StuI StyI TatI Tth111I XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI Bpu10I BpuEI BsaI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TstI XbaI XcmI XmaI