Restriction map for
pFN21AE5019
(Linear) (Six-base) MAPSORT of: KIEE5019.seq Check: 163 from: 1 to: 1050
KIEE5019.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AccI GT'mk_AC
Cuts at: 0 761 1050
Size: 761 289
Acc65I G'GTAC_C
Cuts at: 0 809 1050
Size: 809 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 879 1050
Size: 290 589 171
Fragments arranged by size:
589 290 171
AfeI AGC'GCT
Cuts at: 0 212 1050
Size: 212 838
AflII C'TTAA_G
Cuts at: 0 661 1050
Size: 661 389
AhdI GACnn_n'nnGTC
Cuts at: 0 176 1050
Size: 176 874
AlwNI CAG_nnn'CTG
Cuts at: 0 70 604 774 1050
Size: 70 534 170 276
Fragments arranged by size:
534 276 170 70
AvaI C'yCGr_G
Cuts at: 0 802 1050
Size: 802 248
BanI G'GyrC_C
Cuts at: 0 102 449 809 1050
Size: 102 347 360 241
Fragments arranged by size:
360 347 241 102
BanII G_rGCy'C
Cuts at: 0 94 123 225 1050
Size: 94 29 102 825
Fragments arranged by size:
825 102 94 29
BbeI G_GCGC'C
Cuts at: 0 453 1050
Size: 453 597
BbsI GAAGACnn'nnnn_
Cuts at: 0 885 1050
Size: 885 165
BciVI GTATCCnnnnn_n'
Cuts at: 0 785 1050
Size: 785 265
BlpI GC'TnA_GC
Cuts at: 0 476 1050
Size: 476 574
Bme1580I G_kGCm'C
Cuts at: 0 45 841 1050
Size: 45 796 209
Fragments arranged by size:
796 209 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 784 1050
Size: 784 266
BmtI G_CTAG'C
Cuts at: 0 139 1050
Size: 139 911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 269 1050
Size: 269 781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 239 704 1050
Size: 151 88 465 346
Fragments arranged by size:
465 346 151 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 552 1050
Size: 438 114 498
Fragments arranged by size:
498 438 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 743 1050
Size: 743 307
BsaAI yAC'GTr
Cuts at: 0 387 759 1050
Size: 387 372 291
Fragments arranged by size:
387 372 291
BsaHI Gr'CG_yC
Cuts at: 0 450 1050
Size: 450 600
BsaWI w'CCGG_w
Cuts at: 0 227 1050
Size: 227 823
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 1050
Size: 180 30 840
Fragments arranged by size:
840 180 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 335 459 717 892 964 1050
Size: 283 52 124 258 175 72 86
Fragments arranged by size:
283 258 175 124 86 72 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1050
Size: 367 683
BsiHKAI G_wGCw'C
Cuts at: 0 57 123 392 482 1050
Size: 57 66 269 90 568
Fragments arranged by size:
568 269 90 66 57
Bsp1286I G_dGCh'C
Cuts at: 0 45 57 94 123 225 392 482 841
Size: 45 12 37 29 102 167 90 359
Cuts at: 841 1050
Size: 209
Fragments arranged by size:
359 209 167 102 90 45 37 29
12
BspEI T'CCGG_A
Cuts at: 0 227 1050
Size: 227 823
BsrDI GCAATG_nn'
Cuts at: 0 866 1002 1050
Size: 866 136 48
Fragments arranged by size:
866 136 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 413 1050
Size: 413 637
BstXI CCAn_nnnn'nTGG
Cuts at: 0 853 1050
Size: 853 197
BstYI r'GATC_y
Cuts at: 0 422 665 1050
Size: 422 243 385
Fragments arranged by size:
422 385 243
BtsI GCAGTG_nn'
Cuts at: 0 834 841 1050
Size: 834 7 209
Fragments arranged by size:
834 209 7
EcoICRI GAG'CTC
Cuts at: 0 121 1050
Size: 121 929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 239 290 704 879 1050
Size: 151 88 51 414 175 171
Fragments arranged by size:
414 175 171 151 88 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 686 1050
Size: 686 364
EcoO109I rG'GnC_Cy
Cuts at: 0 458 707 962 1050
Size: 458 249 255 88
Fragments arranged by size:
458 255 249 88
HaeII r_GCGC'y
Cuts at: 0 214 453 1050
Size: 214 239 597
Fragments arranged by size:
597 239 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 1050
Size: 162 32 856
Fragments arranged by size:
856 162 32
KasI G'GCGC_C
Cuts at: 0 449 1050
Size: 449 601
KpnI G_GTAC'C
Cuts at: 0 813 1050
Size: 813 237
MspA1I CmG'CkG
Cuts at: 0 816 1050
Size: 816 234
NarI GG'CG_CC
Cuts at: 0 450 1050
Size: 450 600
NheI G'CTAG_C
Cuts at: 0 135 1050
Size: 135 915
PflMI CCAn_nnn'nTGG
Cuts at: 0 262 774 1050
Size: 262 512 276
Fragments arranged by size:
512 276 262
PfoI T'CCnGG_A
Cuts at: 0 152 767 1050
Size: 152 615 283
Fragments arranged by size:
615 283 152
PmlI CAC'GTG
Cuts at: 0 387 1050
Size: 387 663
PpuMI rG'GwC_Cy
Cuts at: 0 458 707 1050
Size: 458 249 343
Fragments arranged by size:
458 343 249
PstI C_TGCA'G
Cuts at: 0 577 800 829 1048 1050
Size: 577 223 29 219 2
Fragments arranged by size:
577 223 219 29 2
PvuII CAG'CTG
Cuts at: 0 816 1050
Size: 816 234
SacI G_AGCT'C
Cuts at: 0 123 1050
Size: 123 927
SanDI GG'GwC_CC
Cuts at: 0 458 1050
Size: 458 592
SfcI C'TryA_G
Cuts at: 0 235 312 573 796 825 1044 1050
Size: 235 77 261 223 29 219 6
Fragments arranged by size:
261 235 223 219 77 29 6
SfoI GGC'GCC
Cuts at: 0 451 1050
Size: 451 599
SmlI C'TyrA_G
Cuts at: 0 417 531 661 1050
Size: 417 114 130 389
Fragments arranged by size:
417 389 130 114
SnaBI TAC'GTA
Cuts at: 0 759 1050
Size: 759 291
StuI AGG'CCT
Cuts at: 0 1036 1050
Size: 1036 14
StyI C'CwwG_G
Cuts at: 0 439 453 919 1050
Size: 439 14 466 131
Fragments arranged by size:
466 439 131 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 338 1050
Size: 338 712
Tth111I GACn'n_nGTC
Cuts at: 0 66 1050
Size: 66 984
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AhdI AlwNI AvaI
BanI BanII BbeI BbsI BciVI BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BsrDI BstAPI
BstXI BstYI BtsI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI KpnI MspA1I NarI NheI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SanDI SfcI SfoI
SmlI SnaBI StuI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII AclI AflIII AgeI AleI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BglI BglII BmgBI
Bpu10I BsaBI BseYI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI MscI MslI NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SpeI SphI SrfI SspI SwaI TatI TstI
XbaI XcmI XhoI XmaI XmnI ZraI