Restriction map for
pFN21AE5015
(Linear) (Six-base) MAPSORT of: KIEE5015.seq Check: 4880 from: 1 to: 990
KIEE5015.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 990
Size: 651 339
AhdI GACnn_n'nnGTC
Cuts at: 0 121 990
Size: 121 869
AlwNI CAG_nnn'CTG
Cuts at: 0 117 381 990
Size: 117 264 609
Fragments arranged by size:
609 264 117
ApaI G_GGCC'C
Cuts at: 0 975 990
Size: 975 15
ApaLI G'TGCA_C
Cuts at: 0 39 877 990
Size: 39 838 113
Fragments arranged by size:
838 113 39
AvaI C'yCGr_G
Cuts at: 0 85 517 760 795 805 927 933 990
Size: 85 432 243 35 10 122 6 57
Fragments arranged by size:
432 243 122 85 57 35 10 6
BanI G'GyrC_C
Cuts at: 0 410 706 801 990
Size: 410 296 95 189
Fragments arranged by size:
410 296 189 95
BanII G_rGCy'C
Cuts at: 0 212 695 856 934 975 990
Size: 212 483 161 78 41 15
Fragments arranged by size:
483 212 161 78 41 15
BbeI G_GCGC'C
Cuts at: 0 414 710 805 990
Size: 414 296 95 185
Fragments arranged by size:
414 296 185 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 315 858 990
Size: 66 249 543 132
Fragments arranged by size:
543 249 132 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 785 990
Size: 751 34 205
Fragments arranged by size:
751 205 34
BglI GCCn_nnn'nGGC
Cuts at: 0 511 520 990
Size: 511 9 470
Fragments arranged by size:
511 470 9
BlpI GC'TnA_GC
Cuts at: 0 206 990
Size: 206 784
Bme1580I G_kGCm'C
Cuts at: 0 43 881 975 990
Size: 43 838 94 15
Fragments arranged by size:
838 94 43 15
BmgBI CAC'GTC
Cuts at: 0 455 990
Size: 455 535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 205 907 990
Size: 184 21 702 83
Fragments arranged by size:
702 184 83 21
BsaAI yAC'GTr
Cuts at: 0 660 876 990
Size: 660 216 114
Fragments arranged by size:
660 216 114
BsaHI Gr'CG_yC
Cuts at: 0 411 707 802 990
Size: 411 296 95 188
Fragments arranged by size:
411 296 188 95
BsaWI w'CCGG_w
Cuts at: 0 143 990
Size: 143 847
BseYI C'CCAG_C
Cuts at: 0 781 990
Size: 781 209
BsiEI CG_ry'CG
Cuts at: 0 26 108 167 225 990
Size: 26 82 59 58 765
Fragments arranged by size:
765 82 59 58 26
BsiHKAI G_wGCw'C
Cuts at: 0 43 881 990
Size: 43 838 109
Fragments arranged by size:
838 109 43
Bsp1286I G_dGCh'C
Cuts at: 0 43 212 695 856 881 934 975 990
Size: 43 169 483 161 25 53 41 15
Fragments arranged by size:
483 169 161 53 43 41 25 15
BspEI T'CCGG_A
Cuts at: 0 143 990
Size: 143 847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 990
Size: 62 928
BsrBI CCG'CTC
Cuts at: 0 629 845 990
Size: 629 216 145
Fragments arranged by size:
629 216 145
BsrFI r'CCGG_y
Cuts at: 0 220 990
Size: 220 770
BssHII G'CGCG_C
Cuts at: 0 18 765 990
Size: 18 747 225
Fragments arranged by size:
747 225 18
BtgI C'CryG_G
Cuts at: 0 464 813 848 990
Size: 464 349 35 142
Fragments arranged by size:
464 349 142 35
BtsI GCAGTG_nn'
Cuts at: 0 619 990
Size: 619 371
DraIII CAC_nnn'GTG
Cuts at: 0 47 990
Size: 47 943
DrdI GACnn_nn'nnGTC
Cuts at: 0 459 990
Size: 459 531
EaeI y'GGCC_r
Cuts at: 0 23 105 164 222 288 425 482 990
Size: 23 82 59 58 66 137 57 508
Fragments arranged by size:
508 137 82 66 59 58 57 23
EagI C'GGCC_G
Cuts at: 0 23 105 164 222 990
Size: 23 82 59 58 768
Fragments arranged by size:
768 82 59 58 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 846 990
Size: 112 734 144
Fragments arranged by size:
734 144 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 205 651 907 990
Size: 184 21 446 256 83
Fragments arranged by size:
446 256 184 83 21
EcoO109I rG'GnC_Cy
Cuts at: 0 971 990
Size: 971 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 990
Size: 174 816
HaeII r_GCGC'y
Cuts at: 0 68 140 192 199 414 640 710 752
Size: 68 72 52 7 215 226 70 42
Cuts at: 752 805 990
Size: 53 185
Fragments arranged by size:
226 215 185 72 70 68 53 52
42 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 990
Size: 107 32 851
Fragments arranged by size:
851 107 32
KasI G'GCGC_C
Cuts at: 0 410 706 801 990
Size: 410 296 95 189
Fragments arranged by size:
410 296 189 95
MspA1I CmG'CkG
Cuts at: 0 357 990
Size: 357 633
NaeI GCC'GGC
Cuts at: 0 222 990
Size: 222 768
NarI GG'CG_CC
Cuts at: 0 411 707 802 990
Size: 411 296 95 188
Fragments arranged by size:
411 296 188 95
NgoMIV G'CCGG_C
Cuts at: 0 220 990
Size: 220 770
NotI GC'GGCC_GC
Cuts at: 0 105 990
Size: 105 885
NspI r_CATG'y
Cuts at: 0 333 990
Size: 333 657
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 990
Size: 381 609
PmlI CAC'GTG
Cuts at: 0 876 990
Size: 876 114
PshAI GACnn'nnGTC
Cuts at: 0 364 990
Size: 364 626
PspOMI G'GGCC_C
Cuts at: 0 971 990
Size: 971 19
PspXI vC'TCGA_Gb
Cuts at: 0 85 927 990
Size: 85 842 63
Fragments arranged by size:
842 85 63
PstI C_TGCA'G
Cuts at: 0 626 644 990
Size: 626 18 346
Fragments arranged by size:
626 346 18
SexAI A'CCwGG_T
Cuts at: 0 473 719 990
Size: 473 246 271
Fragments arranged by size:
473 271 246
SfcI C'TryA_G
Cuts at: 0 622 640 990
Size: 622 18 350
Fragments arranged by size:
622 350 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 520 990
Size: 520 470
SfoI GGC'GCC
Cuts at: 0 412 708 803 990
Size: 412 296 95 187
Fragments arranged by size:
412 296 187 95
SmaI CCC'GGG
Cuts at: 0 797 935 990
Size: 797 138 55
Fragments arranged by size:
797 138 55
SmlI C'TyrA_G
Cuts at: 0 85 927 990
Size: 85 842 63
Fragments arranged by size:
842 85 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 990
Size: 795 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 471 990
Size: 435 36 519
Fragments arranged by size:
519 435 36
XhoI C'TCGA_G
Cuts at: 0 85 927 990
Size: 85 842 63
Fragments arranged by size:
842 85 63
XmaI C'CCGG_G
Cuts at: 0 795 933 990
Size: 795 138 57
Fragments arranged by size:
795 138 57
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BpmI
BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BssHII BtgI BtsI DraIII DrdI
EaeI EagI EciI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MspA1I NaeI NarI NgoMIV NotI NspI PflMI
PmlI PshAI PspOMI PspXI PstI SexAI SfcI SfiI
SfoI SmaI SmlI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EarI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XmnI ZraI