Restriction map for
pFN21AE5007
(Linear) (Six-base) MAPSORT of: KIEE5007.seq Check: 5371 from: 1 to: 1032
KIEE5007.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 388 1032
Size: 388 644
AccI GT'mk_AC
Cuts at: 0 11 92 1032
Size: 11 81 940
Fragments arranged by size:
940 81 11
AfeI AGC'GCT
Cuts at: 0 58 1032
Size: 58 974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 1032
Size: 299 32 701
Fragments arranged by size:
701 299 32
ApaI G_GGCC'C
Cuts at: 0 217 1032
Size: 217 815
AvaI C'yCGr_G
Cuts at: 0 209 1032
Size: 209 823
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 261 294 1032
Size: 261 33 738
Fragments arranged by size:
738 261 33
BanII G_rGCy'C
Cuts at: 0 179 217 769 1032
Size: 179 38 552 263
Fragments arranged by size:
552 263 179 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 609 1032
Size: 609 423
BciVI GTATCCnnnnn_n'
Cuts at: 0 262 1032
Size: 262 770
BglII A'GATC_T
Cuts at: 0 643 790 985 1032
Size: 643 147 195 47
Fragments arranged by size:
643 195 147 47
Bme1580I G_kGCm'C
Cuts at: 0 217 1032
Size: 217 815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 477 1032
Size: 477 555
Bpu10I CC'TnA_GC
Cuts at: 0 25 1032
Size: 25 1007
BsaI GGTCTCn'nnnn_
Cuts at: 0 596 830 1032
Size: 596 234 202
Fragments arranged by size:
596 234 202
BsaHI Gr'CG_yC
Cuts at: 0 21 140 149 1032
Size: 21 119 9 883
Fragments arranged by size:
883 119 21 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 678 708 1032
Size: 678 30 324
Fragments arranged by size:
678 324 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1032
Size: 73 959
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 1032
Size: 381 651
BsiEI CG_ry'CG
Cuts at: 0 228 1032
Size: 228 804
BsiHKAI G_wGCw'C
Cuts at: 0 179 206 1032
Size: 179 27 826
Fragments arranged by size:
826 179 27
Bsp1286I G_dGCh'C
Cuts at: 0 179 206 217 769 1032
Size: 179 27 11 552 263
Fragments arranged by size:
552 263 179 27 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 812 1032
Size: 388 424 220
Fragments arranged by size:
424 388 220
BstYI r'GATC_y
Cuts at: 0 583 643 790 973 985 1032
Size: 583 60 147 183 12 47
Fragments arranged by size:
583 183 147 60 47 12
BtsI GCAGTG_nn'
Cuts at: 0 261 1032
Size: 261 771
DraI TTT'AAA
Cuts at: 0 377 1032
Size: 377 655
EaeI y'GGCC_r
Cuts at: 0 75 1032
Size: 75 957
EcoICRI GAG'CTC
Cuts at: 0 177 1032
Size: 177 855
EcoO109I rG'GnC_Cy
Cuts at: 0 213 1032
Size: 213 819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 983 1032
Size: 983 49
HaeII r_GCGC'y
Cuts at: 0 60 1032
Size: 60 972
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 678 710 751 783 853 885 1032
Size: 678 32 41 32 70 32 147
Fragments arranged by size:
678 147 70 41 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1032
Size: 259 773
NruI TCG'CGA
Cuts at: 0 224 1032
Size: 224 808
PfoI T'CCnGG_A
Cuts at: 0 38 1032
Size: 38 994
PspOMI G'GGCC_C
Cuts at: 0 213 1032
Size: 213 819
PvuI CG_AT'CG
Cuts at: 0 228 1032
Size: 228 804
SacI G_AGCT'C
Cuts at: 0 179 1032
Size: 179 853
SfcI C'TryA_G
Cuts at: 0 237 1032
Size: 237 795
SmaI CCC'GGG
Cuts at: 0 211 1032
Size: 211 821
SspI AAT'ATT
Cuts at: 0 525 1032
Size: 525 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 818 1032
Size: 818 214
XbaI T'CTAG_A
Cuts at: 0 598 727 982 1032
Size: 598 129 255 50
Fragments arranged by size:
598 255 129 50
XmaI C'CCGG_G
Cuts at: 0 209 1032
Size: 209 823
Enzymes that do cut:
AarI AccI AfeI AloI ApaI AvaI BaeI BanII
BbsI BciVI BglII Bme1580I BplI Bpu10I BsaI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BstYI
BtsI DraI EaeI EcoICRI EcoO109I FalI HaeII Hin4I
MmeI NruI PfoI PspOMI PvuI SacI SfcI SmaI
SspI TaqII XbaI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BpmI BpuEI BsaAI
BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TatI TstI Tth111I XcmI XhoI XmnI ZraI