Restriction map for
pFN21AE5006
(Linear) (Six-base) MAPSORT of: KIEE5006.seq Check: 6609 from: 1 to: 963
KIEE5006.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
Acc65I G'GTAC_C
Cuts at: 0 448 963
Size: 448 515
AclI AA'CG_TT
Cuts at: 0 953 963
Size: 953 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 916 922 963
Size: 637 279 6 41
Fragments arranged by size:
637 279 41 6
AflIII A'CryG_T
Cuts at: 0 362 963
Size: 362 601
AhdI GACnn_n'nnGTC
Cuts at: 0 689 963
Size: 689 274
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 963
Size: 640 32 291
Fragments arranged by size:
640 291 32
AlwNI CAG_nnn'CTG
Cuts at: 0 10 963
Size: 10 953
ApoI r'AATT_y
Cuts at: 0 127 197 963
Size: 127 70 766
Fragments arranged by size:
766 127 70
AseI AT'TA_AT
Cuts at: 0 674 898 963
Size: 674 224 65
Fragments arranged by size:
674 224 65
AvaI C'yCGr_G
Cuts at: 0 164 963
Size: 164 799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 432 465 963
Size: 432 33 498
Fragments arranged by size:
498 432 33
BanI G'GyrC_C
Cuts at: 0 448 963
Size: 448 515
BanII G_rGCy'C
Cuts at: 0 165 963
Size: 165 798
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 963
Size: 110 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 670 704 963
Size: 670 34 259
Fragments arranged by size:
670 259 34
BclI T'GATC_A
Cuts at: 0 369 963
Size: 369 594
BglII A'GATC_T
Cuts at: 0 913 963
Size: 913 50
BmgBI CAC'GTC
Cuts at: 0 363 963
Size: 363 600
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 640 670 963
Size: 400 30 210 30 293
Fragments arranged by size:
400 293 210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 963
Size: 103 860
BsmI GAATG_Cn'
Cuts at: 0 289 963
Size: 289 674
Bsp1286I G_dGCh'C
Cuts at: 0 165 963
Size: 165 798
BspHI T'CATG_A
Cuts at: 0 39 137 963
Size: 39 98 826
Fragments arranged by size:
826 98 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 933 963
Size: 933 30
BsrDI GCAATG_nn'
Cuts at: 0 218 963
Size: 218 745
BsrGI T'GTAC_A
Cuts at: 0 347 963
Size: 347 616
BstYI r'GATC_y
Cuts at: 0 6 913 963
Size: 6 907 50
Fragments arranged by size:
907 50 6
Bsu36I CC'TnA_GG
Cuts at: 0 11 963
Size: 11 952
BtgI C'CryG_G
Cuts at: 0 178 963
Size: 178 785
BtsI GCAGTG_nn'
Cuts at: 0 350 963
Size: 350 613
ClaI AT'CG_AT
Cuts at: 0 530 963
Size: 530 433
DraIII CAC_nnn'GTG
Cuts at: 0 358 963
Size: 358 605
EarI CTCTTCn'nnn_
Cuts at: 0 38 269 963
Size: 38 231 694
Fragments arranged by size:
694 231 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 916 922 963
Size: 637 279 6 41
Fragments arranged by size:
637 279 41 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 675 707 963
Size: 485 32 158 32 256
Fragments arranged by size:
485 256 158 32 32
KpnI G_GTAC'C
Cuts at: 0 452 963
Size: 452 511
MslI CAynn'nnrTG
Cuts at: 0 434 963
Size: 434 529
MspA1I CmG'CkG
Cuts at: 0 228 963
Size: 228 735
NcoI C'CATG_G
Cuts at: 0 178 963
Size: 178 785
NdeI CA'TA_TG
Cuts at: 0 339 963
Size: 339 624
NspI r_CATG'y
Cuts at: 0 820 963
Size: 820 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 963
Size: 258 705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 963
Size: 640 32 291
Fragments arranged by size:
640 291 32
PspXI vC'TCGA_Gb
Cuts at: 0 164 963
Size: 164 799
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 832 864 963
Size: 832 32 99
Fragments arranged by size:
832 99 32
PstI C_TGCA'G
Cuts at: 0 34 683 963
Size: 34 649 280
Fragments arranged by size:
649 280 34
PvuII CAG'CTG
Cuts at: 0 228 963
Size: 228 735
ScaI AGT'ACT
Cuts at: 0 397 963
Size: 397 566
SfcI C'TryA_G
Cuts at: 0 30 284 679 963
Size: 30 254 395 284
Fragments arranged by size:
395 284 254 30
SmlI C'TyrA_G
Cuts at: 0 164 963
Size: 164 799
StyI C'CwwG_G
Cuts at: 0 178 963
Size: 178 785
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 338 963
Size: 338 625
TatI w'GTAC_w
Cuts at: 0 347 395 963
Size: 347 48 568
Fragments arranged by size:
568 347 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 963
Size: 611 32 320
Fragments arranged by size:
611 320 32
XhoI C'TCGA_G
Cuts at: 0 164 963
Size: 164 799
XmnI GAAnn'nnTTC
Cuts at: 0 578 963
Size: 578 385
Enzymes that do cut:
Acc65I AclI AcuI AflIII AhdI AloI AlwNI ApoI
AseI AvaI BaeI BanI BanII BbsI BcgI BclI
BglII BmgBI BsaXI BseRI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstYI Bsu36I BtgI BtsI ClaI DraIII
EarI Eco57MI Hin4I KpnI MslI MspA1I NcoI NdeI
NspI PflMI PpiI PspXI PsrI PstI PvuII ScaI
SfcI SmlI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AleI AlfI
ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI
BciVI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XmaI ZraI