Restriction map for
pFN21AE5003
(Linear) (Six-base) MAPSORT of: KIEE5003.seq Check: 513 from: 1 to: 1401
KIEE5003.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AccI GT'mk_AC
Cuts at: 0 41 1401
Size: 41 1360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 359 1401
Size: 184 175 1042
Fragments arranged by size:
1042 184 175
AfeI AGC'GCT
Cuts at: 0 268 1401
Size: 268 1133
AflII C'TTAA_G
Cuts at: 0 766 1401
Size: 766 635
AflIII A'CryG_T
Cuts at: 0 937 1401
Size: 937 464
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 366 398 1401
Size: 366 32 1003
Fragments arranged by size:
1003 366 32
AlwNI CAG_nnn'CTG
Cuts at: 0 314 552 789 873 1401
Size: 314 238 237 84 528
Fragments arranged by size:
528 314 238 237 84
ApoI r'AATT_y
Cuts at: 0 400 466 496 535 614 922 1401
Size: 400 66 30 39 79 308 479
Fragments arranged by size:
479 400 308 79 66 39 30
AvaI C'yCGr_G
Cuts at: 0 578 1401
Size: 578 823
BbsI GAAGACnn'nnnn_
Cuts at: 0 578 1401
Size: 578 823
BbvCI CC'TCA_GC
Cuts at: 0 6 1401
Size: 6 1395
BmgBI CAC'GTC
Cuts at: 0 1381 1401
Size: 1381 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 895 1378 1401
Size: 574 321 483 23
Fragments arranged by size:
574 483 321 23
Bpu10I CC'TnA_GC
Cuts at: 0 6 1401
Size: 6 1395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 451 1401
Size: 98 353 950
Fragments arranged by size:
950 353 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 116 1401
Size: 116 1285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 6 36 253 283 366 396 1401
Size: 6 30 217 30 83 30 1005
Fragments arranged by size:
1005 217 83 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 1401
Size: 11 1390
BseYI C'CCAG_C
Cuts at: 0 505 1401
Size: 505 896
BsiHKAI G_wGCw'C
Cuts at: 0 1089 1401
Size: 1089 312
Bsp1286I G_dGCh'C
Cuts at: 0 1089 1401
Size: 1089 312
BspHI T'CATG_A
Cuts at: 0 1115 1401
Size: 1115 286
BsrDI GCAATG_nn'
Cuts at: 0 402 509 1401
Size: 402 107 892
Fragments arranged by size:
892 402 107
BsrGI T'GTAC_A
Cuts at: 0 1220 1401
Size: 1220 181
BstYI r'GATC_y
Cuts at: 0 882 1353 1401
Size: 882 471 48
Fragments arranged by size:
882 471 48
BtsI GCAGTG_nn'
Cuts at: 0 211 1401
Size: 211 1190
EaeI y'GGCC_r
Cuts at: 0 442 1401
Size: 442 959
EarI CTCTTCn'nnn_
Cuts at: 0 76 841 1401
Size: 76 765 560
Fragments arranged by size:
765 560 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 359 574 895 1378 1401
Size: 184 175 215 321 483 23
Fragments arranged by size:
483 321 215 184 175 23
EcoRI G'AATT_C
Cuts at: 0 496 1401
Size: 496 905
HaeII r_GCGC'y
Cuts at: 0 270 1401
Size: 270 1131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 223 255 632 664 1401
Size: 148 32 43 32 377 32 737
Fragments arranged by size:
737 377 148 43 32 32 32
HincII GTy'rAC
Cuts at: 0 134 1401
Size: 134 1267
HpaI GTT'AAC
Cuts at: 0 134 1401
Size: 134 1267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1401
Size: 525 876
MscI TGG'CCA
Cuts at: 0 444 1401
Size: 444 957
MslI CAynn'nnrTG
Cuts at: 0 704 1401
Size: 704 697
MspA1I CmG'CkG
Cuts at: 0 147 849 1401
Size: 147 702 552
Fragments arranged by size:
702 552 147
NspI r_CATG'y
Cuts at: 0 941 1401
Size: 941 460
PciI A'CATG_T
Cuts at: 0 937 1401
Size: 937 464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 943 975 1401
Size: 366 32 545 32 426
Fragments arranged by size:
545 426 366 32 32
PvuII CAG'CTG
Cuts at: 0 849 1401
Size: 849 552
SmlI C'TyrA_G
Cuts at: 0 113 430 766 1401
Size: 113 317 336 635
Fragments arranged by size:
635 336 317 113
SspI AAT'ATT
Cuts at: 0 669 731 983 1401
Size: 669 62 252 418
Fragments arranged by size:
669 418 252 62
StyI C'CwwG_G
Cuts at: 0 963 1401
Size: 963 438
TatI w'GTAC_w
Cuts at: 0 864 1220 1401
Size: 864 356 181
Fragments arranged by size:
864 356 181
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AloI AlwNI ApoI
AvaI BbsI BbvCI BmgBI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrGI
BstYI BtsI EaeI EarI Eco57MI EcoRI HaeII Hin4I
HincII HpaI MmeI MscI MslI MspA1I NspI PciI
PpiI PvuII SmlI SspI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HindIII KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI