Restriction map for
pFN21AE4595
(Linear) (Six-base) MAPSORT of: KIEE4595.seq Check: 4085 from: 1 to: 3723
KIEE4595.orf
With 182 enzymes: SgfI *
March 22, 2012 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 293 1944 3723
Size: 293 1651 1779
Fragments arranged by size:
1779 1651 293
AccI GT'mk_AC
Cuts at: 0 3006 3723
Size: 3006 717
Acc65I G'GTAC_C
Cuts at: 0 245 3723
Size: 245 3478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 856 1242 3146 3155 3417 3638 3723
Size: 74 782 386 1904 9 262 221 85
Fragments arranged by size:
1904 782 386 262 221 85 74 9
AflIII A'CryG_T
Cuts at: 0 1246 2723 2755 3723
Size: 1246 1477 32 968
Fragments arranged by size:
1477 1246 968 32
AhdI GACnn_n'nnGTC
Cuts at: 0 727 3723
Size: 727 2996
AleI CACnn'nnGTG
Cuts at: 0 828 2248 2588 2687 3723
Size: 828 1420 340 99 1036
Fragments arranged by size:
1420 1036 828 340 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 1015 3723
Size: 199 816 2708
Fragments arranged by size:
2708 816 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 3723
Size: 553 32 3138
Fragments arranged by size:
3138 553 32
AlwNI CAG_nnn'CTG
Cuts at: 0 505 642 834 1143 1329 1959 2358 3032
Size: 505 137 192 309 186 630 399 674
Cuts at: 3032 3723
Size: 691
Fragments arranged by size:
691 674 630 505 399 309 192 186
137
ApaI G_GGCC'C
Cuts at: 0 108 217 359 3723
Size: 108 109 142 3364
Fragments arranged by size:
3364 142 109 108
ApaLI G'TGCA_C
Cuts at: 0 2206 3723
Size: 2206 1517
ApoI r'AATT_y
Cuts at: 0 277 3723
Size: 277 3446
AvaI C'yCGr_G
Cuts at: 0 92 240 358 800 1376 1529 1674 2026
Size: 92 148 118 442 576 153 145 352
Cuts at: 2026 2307 2594 3171 3476 3723
Size: 281 287 577 305 247
Fragments arranged by size:
577 576 442 352 305 287 281 247
153 148 145 118 92
BamHI G'GATC_C
Cuts at: 0 227 3633 3723
Size: 227 3406 90
Fragments arranged by size:
3406 227 90
BanI G'GyrC_C
Cuts at: 0 245 433 2176 2396 2466 3723
Size: 245 188 1743 220 70 1257
Fragments arranged by size:
1743 1257 245 220 188 70
BanII G_rGCy'C
Cuts at: 0 108 217 359 365 571 625 1421 1791
Size: 108 109 142 6 206 54 796 370
Cuts at: 1791 1889 1906 2109 3382 3723
Size: 98 17 203 1273 341
Fragments arranged by size:
1273 796 370 341 206 203 142 109
108 98 54 17 6
BbeI G_GCGC'C
Cuts at: 0 2400 2470 3723
Size: 2400 70 1253
Fragments arranged by size:
2400 1253 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 932 3143 3304 3626 3723
Size: 932 2211 161 322 97
Fragments arranged by size:
2211 932 322 161 97
BbvCI CC'TCA_GC
Cuts at: 0 307 3281 3706 3723
Size: 307 2974 425 17
Fragments arranged by size:
2974 425 307 17
BglI GCCn_nnn'nGGC
Cuts at: 0 338 2029 2422 3723
Size: 338 1691 393 1301
Fragments arranged by size:
1691 1301 393 338
BlpI GC'TnA_GC
Cuts at: 0 943 1597 3723
Size: 943 654 2126
Fragments arranged by size:
2126 943 654
Bme1580I G_kGCm'C
Cuts at: 0 108 217 359 761 871 2179 2210 2232
Size: 108 109 142 402 110 1308 31 22
Cuts at: 2232 2595 3521 3723
Size: 363 926 202
Fragments arranged by size:
1308 926 402 363 202 142 110 109
108 31 22
BmgBI CAC'GTC
Cuts at: 0 2205 3723
Size: 2205 1518
BmrI ACTGGGnnnn_n'
Cuts at: 0 2331 2341 3723
Size: 2331 10 1382
Fragments arranged by size:
2331 1382 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 400 958 3306 3723
Size: 156 244 558 2348 417
Fragments arranged by size:
2348 558 417 244 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 701 1780 2218 2456 2576 2896 2930
Size: 691 10 1079 438 238 120 320 34
Cuts at: 2930 3146 3578 3723
Size: 216 432 145
Fragments arranged by size:
1079 691 438 432 320 238 216 145
120 34 10
Bpu10I CC'TnA_GC
Cuts at: 0 19 307 816 3281 3706 3723
Size: 19 288 509 2465 425 17
Fragments arranged by size:
2465 509 425 288 19 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 1273 3115 3723
Size: 1273 1842 608
Fragments arranged by size:
1842 1273 608
BsaAI yAC'GTr
Cuts at: 0 468 762 1580 3723
Size: 468 294 818 2143
Fragments arranged by size:
2143 818 468 294
BsaBI GATnn'nnATC
Cuts at: 0 1044 2294 3723
Size: 1044 1250 1429
Fragments arranged by size:
1429 1250 1044
BsaHI Gr'CG_yC
Cuts at: 0 655 1682 2397 2467 3273 3723
Size: 655 1027 715 70 806 450
Fragments arranged by size:
1027 806 715 655 450 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 553 583 775 805 2471 2501
Size: 313 30 210 30 192 30 1666 30
Cuts at: 2501 3337 3367 3451 3481 3723
Size: 836 30 84 30 242
Fragments arranged by size:
1666 836 313 242 210 192 84 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1168 2359 3211 3358 3475 3723
Size: 1168 1191 852 147 117 248
Fragments arranged by size:
1191 1168 852 248 147 117
BseYI C'CCAG_C
Cuts at: 0 454 572 663 1125 1369 3086 3440 3723
Size: 454 118 91 462 244 1717 354 283
Fragments arranged by size:
1717 462 454 354 283 244 118 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1642 2140 2265 3607 3723
Size: 271 1371 498 125 1342 116
Fragments arranged by size:
1371 1342 498 271 125 116
BsiEI CG_ry'CG
Cuts at: 0 342 3723
Size: 342 3381
BsiHKAI G_wGCw'C
Cuts at: 0 365 625 878 989 1522 1889 1906 2210
Size: 365 260 253 111 533 367 17 304
Cuts at: 2210 2525 2675 3382 3723
Size: 315 150 707 341
Fragments arranged by size:
707 533 367 365 341 315 304 260
253 150 111 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1673 3723
Size: 1673 2050
Bsp1286I G_dGCh'C
Cuts at: 0 108 217 359 365 571 625 761 871
Size: 108 109 142 6 206 54 136 110
Cuts at: 871 878 989 1421 1522 1791 1889 1906 2109
Size: 7 111 432 101 269 98 17 203
Cuts at: 2109 2179 2210 2232 2525 2595 2675 3382 3521
Size: 70 31 22 293 70 80 707 139
Cuts at: 3521 3723
Size: 202
Fragments arranged by size:
707 432 293 269 206 203 202 142
139 136 111 110 109 108 101 98
80 70 70 54 31 22 17 7
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 993 1873 1944 3723
Size: 293 700 880 71 1779
Fragments arranged by size:
1779 880 700 293 71
BsrBI CCG'CTC
Cuts at: 0 344 3366 3419 3723
Size: 344 3022 53 304
Fragments arranged by size:
3022 344 304 53
BsrDI GCAATG_nn'
Cuts at: 0 193 2714 3723
Size: 193 2521 1009
Fragments arranged by size:
2521 1009 193
BsrFI r'CCGG_y
Cuts at: 0 849 1722 3723
Size: 849 873 2001
Fragments arranged by size:
2001 873 849
BsrGI T'GTAC_A
Cuts at: 0 2758 3723
Size: 2758 965
BssSI C'ACGA_G
Cuts at: 0 364 3723
Size: 364 3359
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1143 3723
Size: 1143 2580
BstEII G'GTnAC_C
Cuts at: 0 441 1005 1965 3723
Size: 441 564 960 1758
Fragments arranged by size:
1758 960 564 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1614 2689 3124 3723
Size: 1614 1075 435 599
Fragments arranged by size:
1614 1075 599 435
BstYI r'GATC_y
Cuts at: 0 227 1039 2646 2655 3633 3723
Size: 227 812 1607 9 978 90
Fragments arranged by size:
1607 978 812 227 90 9
Bsu36I CC'TnA_GG
Cuts at: 0 3424 3723
Size: 3424 299
BtgI C'CryG_G
Cuts at: 0 437 930 1102 1148 1970 2049 2180 2867
Size: 437 493 172 46 822 79 131 687
Cuts at: 2867 3383 3723
Size: 516 340
Fragments arranged by size:
822 687 516 493 437 340 172 131
79 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 326 2071 3723
Size: 326 1745 1652
Fragments arranged by size:
1745 1652 326
BtsI GCAGTG_nn'
Cuts at: 0 183 823 835 903 997 1666 2157 2583
Size: 183 640 12 68 94 669 491 426
Cuts at: 2583 3723
Size: 1140
Fragments arranged by size:
1140 669 640 491 426 183 94 68
12
DraIII CAC_nnn'GTG
Cuts at: 0 863 2868 2961 3723
Size: 863 2005 93 762
Fragments arranged by size:
2005 863 762 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 3272 3723
Size: 3272 451
EaeI y'GGCC_r
Cuts at: 0 339 739 843 1652 1769 1851 3041 3659
Size: 339 400 104 809 117 82 1190 618
Cuts at: 3659 3723
Size: 64
Fragments arranged by size:
1190 809 618 400 339 117 104 82
64
EagI C'GGCC_G
Cuts at: 0 339 3723
Size: 339 3384
EarI CTCTTCn'nnn_
Cuts at: 0 589 2755 3311 3723
Size: 589 2166 556 412
Fragments arranged by size:
2166 589 556 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1709 1794 3723
Size: 1709 85 1929
Fragments arranged by size:
1929 1709 85
EcoICRI GAG'CTC
Cuts at: 0 363 623 1887 1904 3380 3723
Size: 363 260 1264 17 1476 343
Fragments arranged by size:
1476 1264 363 343 260 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 691 701 856 1242 1780 2218 2456
Size: 74 617 10 155 386 538 438 238
Cuts at: 2456 2576 2896 2930 3146 3155 3417 3578 3638
Size: 120 320 34 216 9 262 161 60
Cuts at: 3638 3723
Size: 85
Fragments arranged by size:
617 538 438 386 320 262 238 216
161 155 120 85 74 60 34 10
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 269 751 2539 2831 3723
Size: 269 482 1788 292 892
Fragments arranged by size:
1788 892 482 292 269
EcoO109I rG'GnC_Cy
Cuts at: 0 105 137 214 261 355 675 721 977
Size: 105 32 77 47 94 320 46 256
Cuts at: 977 1342 1373 2238 2637 2901 2934 3083 3166
Size: 365 31 865 399 264 33 149 83
Cuts at: 3166 3249 3568 3595 3603 3723
Size: 83 319 27 8 120
Fragments arranged by size:
865 399 365 320 319 264 256 149
120 105 94 83 83 77 47 46
33 32 31 27 8
EcoRI G'AATT_C
Cuts at: 0 277 3723
Size: 277 3446
EcoRV GAT'ATC
Cuts at: 0 2378 3723
Size: 2378 1345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1299 2907 3723
Size: 1299 1608 816
Fragments arranged by size:
1608 1299 816
HaeII r_GCGC'y
Cuts at: 0 1883 2400 2438 2470 3723
Size: 1883 517 38 32 1253
Fragments arranged by size:
1883 1253 517 38 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 713 745 1326 1358 2362 2394
Size: 352 32 329 32 581 32 1004 32
Cuts at: 2394 2471 2503 2815 2847 3014 3046 3295 3327
Size: 77 32 312 32 167 32 249 32
Cuts at: 3327 3451 3483 3699 3723
Size: 124 32 216 24
Fragments arranged by size:
1004 581 352 329 312 249 216 167
124 77 32 32 32 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 0 474 1728 3723
Size: 474 1254 1995
Fragments arranged by size:
1995 1254 474
KasI G'GCGC_C
Cuts at: 0 2396 2466 3723
Size: 2396 70 1257
Fragments arranged by size:
2396 1257 70
KpnI G_GTAC'C
Cuts at: 0 249 3723
Size: 249 3474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1538 3293 3723
Size: 1223 315 1755 430
Fragments arranged by size:
1755 1223 430 315
MscI TGG'CCA
Cuts at: 0 741 845 1654 1853 3043 3723
Size: 741 104 809 199 1190 680
Fragments arranged by size:
1190 809 741 680 199 104
MslI CAynn'nnrTG
Cuts at: 0 828 1192 2248 2588 2687 3524 3723
Size: 828 364 1056 340 99 837 199
Fragments arranged by size:
1056 837 828 364 340 199 99
MspA1I CmG'CkG
Cuts at: 0 947 1104 1140 1614 1972 2051 2071 2102
Size: 947 157 36 474 358 79 20 31
Cuts at: 2102 2154 2182 2554 3144 3261 3444 3723
Size: 52 28 372 590 117 183 279
Fragments arranged by size:
947 590 474 372 358 279 183 157
117 79 52 36 31 28 20
NarI GG'CG_CC
Cuts at: 0 2397 2467 3723
Size: 2397 70 1256
Fragments arranged by size:
2397 1256 70
NcoI C'CATG_G
Cuts at: 0 437 930 1148 3723
Size: 437 493 218 2575
Fragments arranged by size:
2575 493 437 218
NspI r_CATG'y
Cuts at: 0 1250 1700 2316 2759 3723
Size: 1250 450 616 443 964
Fragments arranged by size:
1250 964 616 450 443
PasI CC'CwG_GG
Cuts at: 0 6 2937 3514 3599 3723
Size: 6 2931 577 85 124
Fragments arranged by size:
2931 577 124 85 6
PciI A'CATG_T
Cuts at: 0 1246 2755 3723
Size: 1246 1509 968
Fragments arranged by size:
1509 1246 968
PflMI CCAn_nnn'nTGG
Cuts at: 0 863 888 2358 2961 3723
Size: 863 25 1470 603 762
Fragments arranged by size:
1470 863 762 603 25
PfoI T'CCnGG_A
Cuts at: 0 717 2234 3723
Size: 717 1517 1489
Fragments arranged by size:
1517 1489 717
PmlI CAC'GTG
Cuts at: 0 762 1580 3723
Size: 762 818 2143
Fragments arranged by size:
2143 818 762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 3723
Size: 553 32 3138
Fragments arranged by size:
3138 553 32
PpuMI rG'GwC_Cy
Cuts at: 0 261 721 977 1342 1373 2238 2637 3083
Size: 261 460 256 365 31 865 399 446
Cuts at: 3083 3166 3249 3568 3595 3603 3723
Size: 83 83 319 27 8 120
Fragments arranged by size:
865 460 446 399 365 319 261 256
120 83 83 31 27 8
PshAI GACnn'nnGTC
Cuts at: 0 1232 1699 3723
Size: 1232 467 2024
Fragments arranged by size:
2024 1232 467
PspOMI G'GGCC_C
Cuts at: 0 104 213 355 3723
Size: 104 109 142 3368
Fragments arranged by size:
3368 142 109 104
PstI C_TGCA'G
Cuts at: 0 830 975 1004 2553 3723
Size: 830 145 29 1549 1170
Fragments arranged by size:
1549 1170 830 145 29
PvuII CAG'CTG
Cuts at: 0 947 1140 1614 2154 2554 3144 3444 3723
Size: 947 193 474 540 400 590 300 279
Fragments arranged by size:
947 590 540 474 400 300 279 193
SacI G_AGCT'C
Cuts at: 0 365 625 1889 1906 3382 3723
Size: 365 260 1264 17 1476 341
Fragments arranged by size:
1476 1264 365 341 260 17
SacII CC_GC'GG
Cuts at: 0 1105 1973 2052 2183 3723
Size: 1105 868 79 131 1540
Fragments arranged by size:
1540 1105 868 131 79
SanDI GG'GwC_CC
Cuts at: 0 261 977 1342 1373 2637 3083 3568 3595
Size: 261 716 365 31 1264 446 485 27
Cuts at: 3595 3723
Size: 128
Fragments arranged by size:
1264 716 485 446 365 261 128 31
27
SexAI A'CCwGG_T
Cuts at: 0 3653 3723
Size: 3653 70
SfcI C'TryA_G
Cuts at: 0 826 971 1000 2549 3007 3723
Size: 826 145 29 1549 458 716
Fragments arranged by size:
1549 826 716 458 145 29
SfoI GGC'GCC
Cuts at: 0 2398 2468 3723
Size: 2398 70 1255
Fragments arranged by size:
2398 1255 70
SmaI CCC'GGG
Cuts at: 0 94 1378 1676 2309 3723
Size: 94 1284 298 633 1414
Fragments arranged by size:
1414 1284 633 298 94
StuI AGG'CCT
Cuts at: 0 59 298 688 746 1255 2032 3723
Size: 59 239 390 58 509 777 1691
Fragments arranged by size:
1691 777 509 390 239 59 58
StyI C'CwwG_G
Cuts at: 0 437 930 1148 1274 2775 3053 3723
Size: 437 493 218 126 1501 278 670
Fragments arranged by size:
1501 670 493 437 278 218 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 1471 3338 3723
Size: 895 576 1867 385
Fragments arranged by size:
1867 895 576 385
TatI w'GTAC_w
Cuts at: 0 2758 3723
Size: 2758 965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 775 807 3337 3369 3704 3723
Size: 313 32 430 32 2530 32 335 19
Fragments arranged by size:
2530 430 335 313 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 1458 2275 3325 3723
Size: 1458 817 1050 398
Fragments arranged by size:
1458 1050 817 398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1032 3051 3121 3723
Size: 1032 2019 70 602
Fragments arranged by size:
2019 1032 602 70
XmaI C'CCGG_G
Cuts at: 0 92 1376 1674 2307 3723
Size: 92 1284 298 633 1416
Fragments arranged by size:
1416 1284 633 298 92
XmnI GAAnn'nnTTC
Cuts at: 0 583 3723
Size: 583 3140
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI SexAI SfcI SfoI
SmaI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BglII BmtI
BpuEI BsaWI BsiWI BsmI BspEI BspHI BssHII BstBI
BstZ17I ClaI CspCI DraI FseI FspI FspAI HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI