Restriction map for
pFN21AE4591
(Linear) (Six-base) MAPSORT of: KIEE4591.seq Check: 2163 from: 1 to: 3321
KIEE4591.orf
With 182 enzymes: SgfI *
March 22, 2012 18:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1371 3321
Size: 1371 1950
AatII G_ACGT'C
Cuts at: 0 1787 2175 2600 3206 3321
Size: 1787 388 425 606 115
Fragments arranged by size:
1787 606 425 388 115
AccI GT'mk_AC
Cuts at: 0 743 2099 2390 3321
Size: 743 1356 291 931
Fragments arranged by size:
1356 931 743 291
Acc65I G'GTAC_C
Cuts at: 0 599 3321
Size: 599 2722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1558 2098 3321
Size: 850 708 540 1223
Fragments arranged by size:
1223 850 708 540
AfeI AGC'GCT
Cuts at: 0 257 2422 3148 3178 3321
Size: 257 2165 726 30 143
Fragments arranged by size:
2165 726 257 143 30
AgeI A'CCGG_T
Cuts at: 0 1180 3321
Size: 1180 2141
AhdI GACnn_n'nnGTC
Cuts at: 0 2343 3321
Size: 2343 978
AleI CACnn'nnGTG
Cuts at: 0 482 1618 3321
Size: 482 1136 1703
Fragments arranged by size:
1703 1136 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 2995 3268 3321
Size: 935 2060 273 53
Fragments arranged by size:
2060 935 273 53
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 2812 2844 3321
Size: 674 32 2106 32 477
Fragments arranged by size:
2106 674 477 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 1887 1977 2055 2883 3285 3321
Size: 1386 501 90 78 828 402 36
Fragments arranged by size:
1386 828 501 402 90 78 36
ApaI G_GGCC'C
Cuts at: 0 32 54 319 344 473 1230 3321
Size: 32 22 265 25 129 757 2091
Fragments arranged by size:
2091 757 265 129 32 25 22
AvaI C'yCGr_G
Cuts at: 0 31 472 581 1223 1870 2734 3092 3321
Size: 31 441 109 642 647 864 358 229
Fragments arranged by size:
864 647 642 441 358 229 109 31
BanI G'GyrC_C
Cuts at: 0 36 599 1093 2045 2113 3321
Size: 36 563 494 952 68 1208
Fragments arranged by size:
1208 952 563 494 68 36
BanII G_rGCy'C
Cuts at: 0 32 54 278 319 344 473 803 1046
Size: 32 22 224 41 25 129 330 243
Cuts at: 1046 1230 1766 2915 3321
Size: 184 536 1149 406
Fragments arranged by size:
1149 536 406 330 243 224 184 129
41 32 25 22
BbeI G_GCGC'C
Cuts at: 0 2049 2117 3321
Size: 2049 68 1204
Fragments arranged by size:
2049 1204 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 1948 3275 3321
Size: 1948 1327 46
Fragments arranged by size:
1948 1327 46
BbvCI CC'TCA_GC
Cuts at: 0 1627 2306 3321
Size: 1627 679 1015
Fragments arranged by size:
1627 1015 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2607 2641 2914 2948 3321
Size: 2607 34 273 34 373
Fragments arranged by size:
2607 373 273 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1677 3132 3321
Size: 1677 1455 189
Fragments arranged by size:
1677 1455 189
BclI T'GATC_A
Cuts at: 0 1175 2774 3321
Size: 1175 1599 547
Fragments arranged by size:
1599 1175 547
BglI GCCn_nnn'nGGC
Cuts at: 0 339 562 968 1560 2020 2288 2370 3321
Size: 339 223 406 592 460 268 82 951
Fragments arranged by size:
951 592 460 406 339 268 223 82
BglII A'GATC_T
Cuts at: 0 1007 2144 3321
Size: 1007 1137 1177
Fragments arranged by size:
1177 1137 1007
BlpI GC'TnA_GC
Cuts at: 0 937 3055 3321
Size: 937 2118 266
Fragments arranged by size:
2118 937 266
Bme1580I G_kGCm'C
Cuts at: 0 32 41 54 319 344 473 611 712
Size: 32 9 13 265 25 129 138 101
Cuts at: 712 1083 1230 1625 1998 3321
Size: 371 147 395 373 1323
Fragments arranged by size:
1323 395 373 371 265 147 138 129
101 32 25 13 9
BmgBI CAC'GTC
Cuts at: 0 867 882 1173 1464 3321
Size: 867 15 291 291 1857
Fragments arranged by size:
1857 867 291 291 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 557 1897 2349 3321
Size: 557 1340 452 972
Fragments arranged by size:
1340 972 557 452
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 3168 3321
Size: 420 2748 153
Fragments arranged by size:
2748 420 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 817 877 1588 1999 2161 2395 2506
Size: 205 612 60 711 411 162 234 111
Cuts at: 2506 2857 2893 3160 3321
Size: 351 36 267 161
Fragments arranged by size:
711 612 411 351 267 234 205 162
161 111 60 36
Bpu10I CC'TnA_GC
Cuts at: 0 980 986 1627 2306 3321
Size: 980 6 641 679 1015
Fragments arranged by size:
1015 980 679 641 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 3321
Size: 1222 2099
BsaI GGTCTCn'nnnn_
Cuts at: 0 2558 3321
Size: 2558 763
BsaAI yAC'GTr
Cuts at: 0 2700 2772 3321
Size: 2700 72 549
Fragments arranged by size:
2700 549 72
BsaHI Gr'CG_yC
Cuts at: 0 1784 2046 2114 2172 2456 2597 3203 3321
Size: 1784 262 68 58 284 141 606 118
Fragments arranged by size:
1784 606 284 262 141 118 68 58
BsaWI w'CCGG_w
Cuts at: 0 755 897 1180 3321
Size: 755 142 283 2141
Fragments arranged by size:
2141 755 283 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 601 631 1739 1769 2197 2227
Size: 160 30 411 30 1108 30 428 30
Cuts at: 2227 2254 2284 3321
Size: 27 30 1037
Fragments arranged by size:
1108 1037 428 411 160 30 30 30
30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 139 175 178 181 1135 1800 2433
Size: 118 21 36 3 3 954 665 633
Cuts at: 2433 3157 3321
Size: 724 164
Fragments arranged by size:
954 724 665 633 164 118 36 21
3 3
BseYI C'CCAG_C
Cuts at: 0 318 450 715 804 808 1017 1028 1670
Size: 318 132 265 89 4 209 11 642
Cuts at: 1670 2636 2746 3321
Size: 966 110 575
Fragments arranged by size:
966 642 575 318 265 209 132 110
89 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1384 1486 1862 3178 3195 3321
Size: 1123 261 102 376 1316 17 126
Fragments arranged by size:
1316 1123 376 261 126 102 17
BsiEI CG_ry'CG
Cuts at: 0 557 963 2466 2562 2760 3321
Size: 557 406 1503 96 198 561
Fragments arranged by size:
1503 561 557 406 198 96
BsiHKAI G_wGCw'C
Cuts at: 0 374 479 580 803 938 2843 2984 3056
Size: 374 105 101 223 135 1905 141 72
Cuts at: 3056 3321
Size: 265
Fragments arranged by size:
1905 374 265 223 141 135 105 101
72
BsmI GAATG_Cn'
Cuts at: 0 3138 3321
Size: 3138 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 679 3321
Size: 679 2642
Bsp1286I G_dGCh'C
Cuts at: 0 32 41 54 278 319 344 374 473
Size: 32 9 13 224 41 25 30 99
Cuts at: 473 479 580 611 712 803 938 1046 1083
Size: 6 101 31 101 91 135 108 37
Cuts at: 1083 1230 1625 1766 1998 2843 2915 2984 3056
Size: 147 395 141 232 845 72 69 72
Cuts at: 3056 3321
Size: 265
Fragments arranged by size:
845 395 265 232 224 147 141 135
108 101 101 99 91 72 72 69
41 37 32 31 30 25 13 9
6
BspHI T'CATG_A
Cuts at: 0 1829 3321
Size: 1829 1492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 497 1136 1371 1496 1559 1643 1644
Size: 195 302 639 235 125 63 84 1
Cuts at: 1644 1994 2411 2618 3321
Size: 350 417 207 703
Fragments arranged by size:
703 639 417 350 302 235 207 195
125 84 63 1
BsrBI CCG'CTC
Cuts at: 0 990 1409 1572 3321
Size: 990 419 163 1749
Fragments arranged by size:
1749 990 419 163
BsrDI GCAATG_nn'
Cuts at: 0 917 3321
Size: 917 2404
BsrFI r'CCGG_y
Cuts at: 0 552 958 1180 1273 1325 2683 3321
Size: 552 406 222 93 52 1358 638
Fragments arranged by size:
1358 638 552 406 222 93 52
BssSI C'ACGA_G
Cuts at: 0 655 1693 2515 3196 3321
Size: 655 1038 822 681 125
Fragments arranged by size:
1038 822 681 655 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1386 1689 2474 2847 3032 3041 3285 3321
Size: 1386 303 785 373 185 9 244 36
Fragments arranged by size:
1386 785 373 303 244 185 36 9
BstEII G'GTnAC_C
Cuts at: 0 387 484 3321
Size: 387 97 2837
Fragments arranged by size:
2837 387 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1833 2199 2373 2796 3321
Size: 1833 366 174 423 525
Fragments arranged by size:
1833 525 423 366 174
BstYI r'GATC_y
Cuts at: 0 225 752 1007 1973 2144 3321
Size: 225 527 255 966 171 1177
Fragments arranged by size:
1177 966 527 255 225 171
BstZ17I GTA'TAC
Cuts at: 0 2100 3321
Size: 2100 1221
Bsu36I CC'TnA_GG
Cuts at: 0 823 2570 3311 3321
Size: 823 1747 741 10
Fragments arranged by size:
1747 823 741 10
BtgI C'CryG_G
Cuts at: 0 54 354 423 603 638 907 1595 1712
Size: 54 300 69 180 35 269 688 117
Cuts at: 1712 2333 3321
Size: 621 988
Fragments arranged by size:
988 688 621 300 269 180 117 69
54 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1776 2326 3321
Size: 1776 550 995
Fragments arranged by size:
1776 995 550
BtsI GCAGTG_nn'
Cuts at: 0 853 1555 2531 2539 3175 3321
Size: 853 702 976 8 636 146
Fragments arranged by size:
976 853 702 636 146 8
ClaI AT'CG_AT
Cuts at: 0 2627 3321
Size: 2627 694
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 3321
Size: 205 3116
EaeI y'GGCC_r
Cuts at: 0 16 88 509 554 960 1271 1647 2021
Size: 16 72 421 45 406 311 376 374
Cuts at: 2021 2519 2708 2798 2885 3321
Size: 498 189 90 87 436
Fragments arranged by size:
498 436 421 406 376 374 311 189
90 87 72 45 16
EagI C'GGCC_G
Cuts at: 0 554 960 3321
Size: 554 406 2361
Fragments arranged by size:
2361 554 406
EarI CTCTTCn'nnn_
Cuts at: 0 1305 1996 2443 3321
Size: 1305 691 447 878
Fragments arranged by size:
1305 878 691 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 877 3321
Size: 877 2444
EcoICRI GAG'CTC
Cuts at: 0 801 3321
Size: 801 2520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 817 850 877 1558 1588 1999 2098
Size: 205 612 33 27 681 30 411 99
Cuts at: 2098 2161 2395 2506 2857 2893 3160 3321
Size: 63 234 111 351 36 267 161
Fragments arranged by size:
681 612 411 351 267 234 205 161
111 99 63 36 33 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 1132 1897 2923 3196 3321
Size: 682 450 765 1026 273 125
Fragments arranged by size:
1026 765 682 450 273 125
EcoO109I rG'GnC_Cy
Cuts at: 0 28 60 108 207 340 341 993 1125
Size: 28 32 48 99 133 1 652 132
Cuts at: 1125 1916 2052 2481 2730 2820 3261 3321
Size: 791 136 429 249 90 441 60
Fragments arranged by size:
791 652 441 429 249 136 133 132
99 90 60 48 32 28 1
EcoRV GAT'ATC
Cuts at: 0 948 2631 3321
Size: 948 1683 690
Fragments arranged by size:
1683 948 690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2409 3321
Size: 2409 912
FseI GG_CCGG'CC
Cuts at: 0 556 1277 3321
Size: 556 721 2044
Fragments arranged by size:
2044 721 556
FspI TGC'GCA
Cuts at: 0 631 2497 2902 3244 3321
Size: 631 1866 405 342 77
Fragments arranged by size:
1866 631 405 342 77
HaeII r_GCGC'y
Cuts at: 0 259 457 646 1063 2049 2077 2090 2117
Size: 259 198 189 417 986 28 13 27
Cuts at: 2117 2424 3150 3180 3294 3321
Size: 307 726 30 114 27
Fragments arranged by size:
986 726 417 307 259 198 189 114
30 28 27 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 1739 1771 1795 1827 1887 1919
Size: 160 32 1547 32 24 32 60 32
Cuts at: 1919 2254 2286 2329 2361 2587 2619 3321
Size: 335 32 43 32 226 32 702
Fragments arranged by size:
1547 702 335 226 160 60 43 32
32 32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 0 744 3321
Size: 744 2577
KasI G'GCGC_C
Cuts at: 0 2045 2113 3321
Size: 2045 68 1208
Fragments arranged by size:
2045 1208 68
KpnI G_GTAC'C
Cuts at: 0 603 3321
Size: 603 2718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 312 1310 2699 2795 3321
Size: 275 37 998 1389 96 526
Fragments arranged by size:
1389 998 526 275 96 37
MscI TGG'CCA
Cuts at: 0 511 1649 2023 2521 2887 3321
Size: 511 1138 374 498 366 434
Fragments arranged by size:
1138 511 498 434 374 366
MslI CAynn'nnrTG
Cuts at: 0 482 637 694 1618 1831 3131 3321
Size: 482 155 57 924 213 1300 190
Fragments arranged by size:
1300 924 482 213 190 155 57
MspA1I CmG'CkG
Cuts at: 0 808 855 1674 2070 2199 2335 2640 2826
Size: 808 47 819 396 129 136 305 186
Cuts at: 2826 2883 3321
Size: 57 438
Fragments arranged by size:
819 808 438 396 305 186 136 129
57 47
NaeI GCC'GGC
Cuts at: 0 554 960 1275 1327 2685 3321
Size: 554 406 315 52 1358 636
Fragments arranged by size:
1358 636 554 406 315 52
NarI GG'CG_CC
Cuts at: 0 2046 2114 3321
Size: 2046 68 1207
Fragments arranged by size:
2046 1207 68
NcoI C'CATG_G
Cuts at: 0 907 3321
Size: 907 2414
NgoMIV G'CCGG_C
Cuts at: 0 552 958 1273 1325 2683 3321
Size: 552 406 315 52 1358 638
Fragments arranged by size:
1358 638 552 406 315 52
NspI r_CATG'y
Cuts at: 0 594 1377 3243 3321
Size: 594 783 1866 78
Fragments arranged by size:
1866 783 594 78
PasI CC'CwG_GG
Cuts at: 0 25 2319 3321
Size: 25 2294 1002
Fragments arranged by size:
2294 1002 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 294 312 1443 1977 2055 3321
Size: 294 18 1131 534 78 1266
Fragments arranged by size:
1266 1131 534 294 78 18
PfoI T'CCnGG_A
Cuts at: 0 182 677 1292 1976 2054 3321
Size: 182 495 615 684 78 1267
Fragments arranged by size:
1267 684 615 495 182 78
PmlI CAC'GTG
Cuts at: 0 2700 3321
Size: 2700 621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1891 1923 3321
Size: 742 32 1117 32 1398
Fragments arranged by size:
1398 1117 742 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 108 207 993 1125 1916 2052 2730 2820
Size: 108 99 786 132 791 136 678 90
Cuts at: 2820 3261 3321
Size: 441 60
Fragments arranged by size:
791 786 678 441 136 132 108 99
90 60
PspOMI G'GGCC_C
Cuts at: 0 28 50 315 340 469 1226 3321
Size: 28 22 265 25 129 757 2095
Fragments arranged by size:
2095 757 265 129 28 25 22
PstI C_TGCA'G
Cuts at: 0 152 176 1136 1382 1493 3321
Size: 152 24 960 246 111 1828
Fragments arranged by size:
1828 960 246 152 111 24
PvuII CAG'CTG
Cuts at: 0 808 855 1674 2070 2199 2640 2883 3321
Size: 808 47 819 396 129 441 243 438
Fragments arranged by size:
819 808 441 438 396 243 129 47
SacI G_AGCT'C
Cuts at: 0 803 3321
Size: 803 2518
SacII CC_GC'GG
Cuts at: 0 2336 3321
Size: 2336 985
SalI G'TCGA_C
Cuts at: 0 742 3321
Size: 742 2579
SanDI GG'GwC_CC
Cuts at: 0 993 2730 3321
Size: 993 1737 591
Fragments arranged by size:
1737 993 591
SapI GCTCTTCn'nnn_
Cuts at: 0 2443 3321
Size: 2443 878
ScaI AGT'ACT
Cuts at: 0 2866 3321
Size: 2866 455
SexAI A'CCwGG_T
Cuts at: 0 2525 3321
Size: 2525 796
SfcI C'TryA_G
Cuts at: 0 148 172 1132 1378 1489 2232 3321
Size: 148 24 960 246 111 743 1089
Fragments arranged by size:
1089 960 743 246 148 111 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2020 3321
Size: 2020 1301
SfoI GGC'GCC
Cuts at: 0 2047 2115 3321
Size: 2047 68 1206
Fragments arranged by size:
2047 1206 68
SmaI CCC'GGG
Cuts at: 0 33 1872 2736 3094 3321
Size: 33 1839 864 358 227
Fragments arranged by size:
1839 864 358 227 33
SmlI C'TyrA_G
Cuts at: 0 1237 3321
Size: 1237 2084
StuI AGG'CCT
Cuts at: 0 704 1138 3316 3321
Size: 704 434 2178 5
Fragments arranged by size:
2178 704 434 5
StyI C'CwwG_G
Cuts at: 0 907 1097 1337 2009 2024 2246 2651 2789
Size: 907 190 240 672 15 222 405 138
Cuts at: 2789 3321
Size: 532
Fragments arranged by size:
907 672 532 405 240 222 190 138
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 908 1081 2331 2576 3321
Size: 472 436 173 1250 245 745
Fragments arranged by size:
1250 745 472 436 245 173
TatI w'GTAC_w
Cuts at: 0 1397 1529 2213 2864 3321
Size: 1397 132 684 651 457
Fragments arranged by size:
1397 684 651 457 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 856 888 2599 2631 3321
Size: 601 32 223 32 1711 32 690
Fragments arranged by size:
1711 690 601 223 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 778 883 997 2272 2761 3321
Size: 778 105 114 1275 489 560
Fragments arranged by size:
1275 778 560 489 114 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1797 2016 2056 2199 2247 3321
Size: 1797 219 40 143 48 1074
Fragments arranged by size:
1797 1074 219 143 48 40
XmaI C'CCGG_G
Cuts at: 0 31 1870 2734 3092 3321
Size: 31 1839 864 358 229
Fragments arranged by size:
1839 864 358 229 31
ZraI GAC'GTC
Cuts at: 0 1785 2173 2598 3204 3321
Size: 1785 388 425 606 117
Fragments arranged by size:
1785 606 425 388 117
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AflII AflIII ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BfrBI BmtI BsaBI BsiWI BspEI
BsrGI BssHII BstBI CspCI DraI DraIII EcoRI FspAI
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SbfI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI