Restriction map for
pFN21AE4590
(Linear) (Six-base) MAPSORT of: KIEE4590.seq Check: 602 from: 1 to: 3282
KIEE4590.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 871 3282
Size: 871 2411
Acc65I G'GTAC_C
Cuts at: 0 1895 3282
Size: 1895 1387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 391 907 1010 1135 1183 2276 2549
Size: 338 53 516 103 125 48 1093 273
Cuts at: 2549 3205 3280 3282
Size: 656 75 2
Fragments arranged by size:
1093 656 516 338 273 125 103 75
53 48 2
AfeI AGC'GCT
Cuts at: 0 402 3282
Size: 402 2880
AflIII A'CryG_T
Cuts at: 0 1319 3282
Size: 1319 1963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 350 2803 3282
Size: 350 2453 479
Fragments arranged by size:
2453 479 350
AlwNI CAG_nnn'CTG
Cuts at: 0 2362 3282
Size: 2362 920
ApaLI G'TGCA_C
Cuts at: 0 3148 3282
Size: 3148 134
ApoI r'AATT_y
Cuts at: 0 813 1172 1222 2007 3045 3282
Size: 813 359 50 785 1038 237
Fragments arranged by size:
1038 813 785 359 237 50
AvaI C'yCGr_G
Cuts at: 0 90 3282
Size: 90 3192
BamHI G'GATC_C
Cuts at: 0 879 2827 3282
Size: 879 1948 455
Fragments arranged by size:
1948 879 455
BanI G'GyrC_C
Cuts at: 0 1858 1895 1926 3282
Size: 1858 37 31 1356
Fragments arranged by size:
1858 1356 37 31
BanII G_rGCy'C
Cuts at: 0 369 3282
Size: 369 2913
BbeI G_GCGC'C
Cuts at: 0 1930 3282
Size: 1930 1352
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 2333 3282
Size: 806 1527 949
Fragments arranged by size:
1527 949 806
BciVI GTATCCnnnnn_n'
Cuts at: 0 2490 3282
Size: 2490 792
BclI T'GATC_A
Cuts at: 0 834 1151 1692 3282
Size: 834 317 541 1590
Fragments arranged by size:
1590 834 541 317
BfrBI ATG'CAT
Cuts at: 0 226 2790 3282
Size: 226 2564 492
Fragments arranged by size:
2564 492 226
BglI GCCn_nnn'nGGC
Cuts at: 0 1331 3282
Size: 1331 1951
BglII A'GATC_T
Cuts at: 0 135 657 2457 3282
Size: 135 522 1800 825
Fragments arranged by size:
1800 825 522 135
BlpI GC'TnA_GC
Cuts at: 0 959 3282
Size: 959 2323
Bme1580I G_kGCm'C
Cuts at: 0 3152 3282
Size: 3152 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 1659 1901 2470 3282
Size: 1659 242 569 812
Fragments arranged by size:
1659 812 569 242
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 2067 2175 3282
Size: 90 1977 108 1107
Fragments arranged by size:
1977 1107 108 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2145 2471 3282
Size: 2145 326 811
Fragments arranged by size:
2145 811 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1840 2836 3282
Size: 1840 996 446
Fragments arranged by size:
1840 996 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 1706 2703 3282
Size: 1706 997 579
Fragments arranged by size:
1706 997 579
BsaAI yAC'GTr
Cuts at: 0 1322 2613 3282
Size: 1322 1291 669
Fragments arranged by size:
1322 1291 669
BsaBI GATnn'nnATC
Cuts at: 0 833 884 1841 3282
Size: 833 51 957 1441
Fragments arranged by size:
1441 957 833 51
BsaHI Gr'CG_yC
Cuts at: 0 1927 3282
Size: 1927 1355
BsaWI w'CCGG_w
Cuts at: 0 34 3282
Size: 34 3248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1033 1063 3282
Size: 1033 30 2219
Fragments arranged by size:
2219 1033 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 2059 2148 2420 3282
Size: 53 2006 89 272 862
Fragments arranged by size:
2006 862 272 89 53
BseYI C'CCAG_C
Cuts at: 0 57 1983 3203 3282
Size: 57 1926 1220 79
Fragments arranged by size:
1926 1220 79 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1403 1793 3020 3119 3282
Size: 1066 337 390 1227 99 163
Fragments arranged by size:
1227 1066 390 337 163 99
BsiHKAI G_wGCw'C
Cuts at: 0 369 3152 3282
Size: 369 2783 130
Fragments arranged by size:
2783 369 130
BsmI GAATG_Cn'
Cuts at: 0 510 939 3282
Size: 510 429 2343
Fragments arranged by size:
2343 510 429
Bsp1286I G_dGCh'C
Cuts at: 0 369 3152 3282
Size: 369 2783 130
Fragments arranged by size:
2783 369 130
BspEI T'CCGG_A
Cuts at: 0 34 3282
Size: 34 3248
BspHI T'CATG_A
Cuts at: 0 449 3282
Size: 449 2833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 711 1425 3282
Size: 711 714 1857
Fragments arranged by size:
1857 714 711
BsrDI GCAATG_nn'
Cuts at: 0 2142 3029 3282
Size: 2142 887 253
Fragments arranged by size:
2142 887 253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 514 2994 3282
Size: 514 2480 288
Fragments arranged by size:
2480 514 288
BstBI TT'CG_AA
Cuts at: 0 1944 3282
Size: 1944 1338
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 1458 3282
Size: 1305 153 1824
Fragments arranged by size:
1824 1305 153
BstYI r'GATC_y
Cuts at: 0 135 330 657 879 2457 2827 3282
Size: 135 195 327 222 1578 370 455
Fragments arranged by size:
1578 455 370 327 222 195 135
BstZ17I GTA'TAC
Cuts at: 0 872 3282
Size: 872 2410
Bsu36I CC'TnA_GG
Cuts at: 0 2822 3282
Size: 2822 460
BtsI GCAGTG_nn'
Cuts at: 0 621 1813 2073 2154 3282
Size: 621 1192 260 81 1128
Fragments arranged by size:
1192 1128 621 260 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2472 2507 3282
Size: 2472 35 775
Fragments arranged by size:
2472 775 35
DraI TTT'AAA
Cuts at: 0 1833 3282
Size: 1833 1449
EaeI y'GGCC_r
Cuts at: 0 260 1448 1915 2957 3111 3282
Size: 260 1188 467 1042 154 171
Fragments arranged by size:
1188 1042 467 260 171 154
EarI CTCTTCn'nnn_
Cuts at: 0 704 1111 1217 2036 2058 2379 2568 3282
Size: 704 407 106 819 22 321 189 714
Fragments arranged by size:
819 714 704 407 321 189 106 22
EcoICRI GAG'CTC
Cuts at: 0 367 3282
Size: 367 2915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 391 907 1010 1135 1183 2145 2276
Size: 338 53 516 103 125 48 962 131
Cuts at: 2276 2471 2549 3205 3280 3282
Size: 195 78 656 75 2
Fragments arranged by size:
962 656 516 338 195 131 125 103
78 75 53 48 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3274 3282
Size: 3274 8
EcoO109I rG'GnC_Cy
Cuts at: 0 95 375 3282
Size: 95 280 2907
Fragments arranged by size:
2907 280 95
EcoRI G'AATT_C
Cuts at: 0 2007 3282
Size: 2007 1275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2690 3282
Size: 2690 592
HaeII r_GCGC'y
Cuts at: 0 404 1930 3282
Size: 404 1526 1352
Fragments arranged by size:
1526 1352 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1596 1628 2068 2100 2332 2364 2517 2549
Size: 1596 32 440 32 232 32 153 32
Cuts at: 2549 3282
Size: 733
Fragments arranged by size:
1596 733 440 232 153 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1136 2555 3282
Size: 1136 1419 727
Fragments arranged by size:
1419 1136 727
HindIII A'AGCT_T
Cuts at: 0 470 1165 1569 3282
Size: 470 695 404 1713
Fragments arranged by size:
1713 695 470 404
KasI G'GCGC_C
Cuts at: 0 1926 3282
Size: 1926 1356
KpnI G_GTAC'C
Cuts at: 0 1899 3282
Size: 1899 1383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1376 2916 3282
Size: 1168 208 1540 366
Fragments arranged by size:
1540 1168 366 208
MscI TGG'CCA
Cuts at: 0 262 1450 1917 2959 3113 3282
Size: 262 1188 467 1042 154 169
Fragments arranged by size:
1188 1042 467 262 169 154
MslI CAynn'nnrTG
Cuts at: 0 2785 3282
Size: 2785 497
MspA1I CmG'CkG
Cuts at: 0 963 3282
Size: 963 2319
NarI GG'CG_CC
Cuts at: 0 1927 3282
Size: 1927 1355
NdeI CA'TA_TG
Cuts at: 0 492 1445 3282
Size: 492 953 1837
Fragments arranged by size:
1837 953 492
NsiI A_TGCA'T
Cuts at: 0 228 2792 3282
Size: 228 2564 490
Fragments arranged by size:
2564 490 228
NspI r_CATG'y
Cuts at: 0 741 2794 3282
Size: 741 2053 488
Fragments arranged by size:
2053 741 488
PflMI CCAn_nnn'nTGG
Cuts at: 0 2935 3282
Size: 2935 347
PfoI T'CCnGG_A
Cuts at: 0 1457 3282
Size: 1457 1825
PmlI CAC'GTG
Cuts at: 0 1322 2613 3282
Size: 1322 1291 669
Fragments arranged by size:
1322 1291 669
PpuMI rG'GwC_Cy
Cuts at: 0 375 3282
Size: 375 2907
PshAI GACnn'nnGTC
Cuts at: 0 2190 3282
Size: 2190 1092
PsiI TTA'TAA
Cuts at: 0 1393 3282
Size: 1393 1889
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2418 2450 2933 2965 3282
Size: 2418 32 483 32 317
Fragments arranged by size:
2418 483 317 32 32
PstI C_TGCA'G
Cuts at: 0 1604 3282
Size: 1604 1678
PvuII CAG'CTG
Cuts at: 0 963 3282
Size: 963 2319
SacI G_AGCT'C
Cuts at: 0 369 3282
Size: 369 2913
ScaI AGT'ACT
Cuts at: 0 2583 3282
Size: 2583 699
SexAI A'CCwGG_T
Cuts at: 0 302 3282
Size: 302 2980
SfcI C'TryA_G
Cuts at: 0 1280 1600 2774 3197 3282
Size: 1280 320 1174 423 85
Fragments arranged by size:
1280 1174 423 320 85
SfoI GGC'GCC
Cuts at: 0 1928 3282
Size: 1928 1354
SmlI C'TyrA_G
Cuts at: 0 1819 2815 3282
Size: 1819 996 467
Fragments arranged by size:
1819 996 467
SpeI A'CTAG_T
Cuts at: 0 696 1014 1576 3282
Size: 696 318 562 1706
Fragments arranged by size:
1706 696 562 318
SspI AAT'ATT
Cuts at: 0 273 1790 2922 3282
Size: 273 1517 1132 360
Fragments arranged by size:
1517 1132 360 273
StuI AGG'CCT
Cuts at: 0 2198 3282
Size: 2198 1084
StyI C'CwwG_G
Cuts at: 0 775 2897 3282
Size: 775 2122 385
Fragments arranged by size:
2122 775 385
TatI w'GTAC_w
Cuts at: 0 1667 2581 3282
Size: 1667 914 701
Fragments arranged by size:
1667 914 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 771 803 3282
Size: 771 32 2479
Fragments arranged by size:
2479 771 32
XbaI T'CTAG_A
Cuts at: 0 2454 3282
Size: 2454 828
XmnI GAAnn'nnTTC
Cuts at: 0 236 581 1120 1526 3282
Size: 236 345 539 406 1756
Fragments arranged by size:
1756 539 406 345 236
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NarI NdeI NsiI
NspI PflMI PfoI PmlI PpuMI PshAI PsiI PsrI
PstI PvuII SacI ScaI SexAI SfcI SfoI SmlI
SpeI SspI StuI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BmgBI BmtI Bpu10I BsiEI BsiWI BsmBI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BtgI BtgZI ClaI DraIII
DrdI EagI EciI EcoRV FseI FspI FspAI HpaI
MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PpiI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SapI SbfI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I
XcmI XhoI XmaI ZraI