Restriction map for
pFN21AE4588
(Linear) (Six-base) MAPSORT of: KIEE4588.seq Check: 9277 from: 1 to: 2739
KIEE4588.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AclI AA'CG_TT
Cuts at: 0 1648 2739
Size: 1648 1091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1217 1354 1422 1624 2095 2146 2739
Size: 931 286 137 68 202 471 51 593
Fragments arranged by size:
931 593 471 286 202 137 68 51
AflII C'TTAA_G
Cuts at: 0 2656 2739
Size: 2656 83
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1448 1480 2739
Size: 1448 32 1259
Fragments arranged by size:
1448 1259 32
AlwNI CAG_nnn'CTG
Cuts at: 0 94 322 2739
Size: 94 228 2417
Fragments arranged by size:
2417 228 94
ApoI r'AATT_y
Cuts at: 0 951 1136 1406 1615 2203 2632 2739
Size: 951 185 270 209 588 429 107
Fragments arranged by size:
951 588 429 270 209 185 107
AseI AT'TA_AT
Cuts at: 0 487 1210 1318 1520 2739
Size: 487 723 108 202 1219
Fragments arranged by size:
1219 723 487 202 108
AvrII C'CTAG_G
Cuts at: 0 2136 2739
Size: 2136 603
BanII G_rGCy'C
Cuts at: 0 1002 2739
Size: 1002 1737
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 371 2739
Size: 227 144 2368
Fragments arranged by size:
2368 227 144
BbvCI CC'TCA_GC
Cuts at: 0 87 404 2739
Size: 87 317 2335
Fragments arranged by size:
2335 317 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2052 2086 2739
Size: 2052 34 653
Fragments arranged by size:
2052 653 34
BclI T'GATC_A
Cuts at: 0 593 899 905 1014 1772 2739
Size: 593 306 6 109 758 967
Fragments arranged by size:
967 758 593 306 109 6
BglII A'GATC_T
Cuts at: 0 115 785 2739
Size: 115 670 1954
Fragments arranged by size:
1954 670 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 2739
Size: 74 2665
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 429 2739
Size: 429 2310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1006 2212 2739
Size: 394 612 1206 527
Fragments arranged by size:
1206 612 527 394
Bpu10I CC'TnA_GC
Cuts at: 0 87 104 404 2739
Size: 87 17 300 2335
Fragments arranged by size:
2335 300 87 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 2739
Size: 1768 971
BsaI GGTCTCn'nnnn_
Cuts at: 0 2238 2739
Size: 2238 501
BsaAI yAC'GTr
Cuts at: 0 336 2739
Size: 336 2403
BsaBI GATnn'nnATC
Cuts at: 0 904 2464 2739
Size: 904 1560 275
Fragments arranged by size:
1560 904 275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1448 1478 2739
Size: 1448 30 1261
Fragments arranged by size:
1448 1261 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 234 245 405 408 411 414 417 2293
Size: 234 11 160 3 3 3 3 1876
Cuts at: 2293 2739
Size: 446
Fragments arranged by size:
1876 446 234 160 11 3 3 3
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1736 2739
Size: 1045 691 1003
Fragments arranged by size:
1045 1003 691
BsmI GAATG_Cn'
Cuts at: 0 1046 1912 2739
Size: 1046 866 827
Fragments arranged by size:
1046 866 827
BsmBI CGTCTCn'nnnn_
Cuts at: 0 552 2739
Size: 552 2187
Bsp1286I G_dGCh'C
Cuts at: 0 1002 2739
Size: 1002 1737
BspHI T'CATG_A
Cuts at: 0 902 1973 2739
Size: 902 1071 766
Fragments arranged by size:
1071 902 766
BsrDI GCAATG_nn'
Cuts at: 0 885 2739
Size: 885 1854
BsrFI r'CCGG_y
Cuts at: 0 1181 2739
Size: 1181 1558
BsrGI T'GTAC_A
Cuts at: 0 767 2618 2739
Size: 767 1851 121
Fragments arranged by size:
1851 767 121
BstBI TT'CG_AA
Cuts at: 0 1104 2565 2739
Size: 1104 1461 174
Fragments arranged by size:
1461 1104 174
BstYI r'GATC_y
Cuts at: 0 115 785 2739
Size: 115 670 1954
Fragments arranged by size:
1954 670 115
BtgI C'CryG_G
Cuts at: 0 1541 2739
Size: 1541 1198
BtsI GCAGTG_nn'
Cuts at: 0 674 1483 2341 2739
Size: 674 809 858 398
Fragments arranged by size:
858 809 674 398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2130 2165 2739
Size: 2130 35 574
Fragments arranged by size:
2130 574 35
DraI TTT'AAA
Cuts at: 0 606 857 1382 1566 1947 2208 2739
Size: 606 251 525 184 381 261 531
Fragments arranged by size:
606 531 525 381 261 251 184
DraIII CAC_nnn'GTG
Cuts at: 0 2190 2739
Size: 2190 549
EaeI y'GGCC_r
Cuts at: 0 2059 2739
Size: 2059 680
EarI CTCTTCn'nnn_
Cuts at: 0 160 243 252 498 1427 1441 2288 2656
Size: 160 83 9 246 929 14 847 368
Cuts at: 2656 2739
Size: 83
Fragments arranged by size:
929 847 368 246 160 83 83 14
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 137 2739
Size: 137 2602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 931 1006 1217 1354 1422 1624 2095
Size: 394 537 75 211 137 68 202 471
Cuts at: 2095 2146 2212 2739
Size: 51 66 527
Fragments arranged by size:
537 527 471 394 211 202 137 75
68 66 51
EcoRI G'AATT_C
Cuts at: 0 951 1406 2739
Size: 951 455 1333
Fragments arranged by size:
1333 951 455
EcoRV GAT'ATC
Cuts at: 0 1593 2739
Size: 1593 1146
HaeII r_GCGC'y
Cuts at: 0 1842 2739
Size: 1842 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 220 252 265 297 884 916
Size: 100 32 88 32 13 32 587 32
Cuts at: 916 2739
Size: 1823
Fragments arranged by size:
1823 587 100 88 32 32 32 32
13
HincII GTy'rAC
Cuts at: 0 1251 1578 2030 2174 2739
Size: 1251 327 452 144 565
Fragments arranged by size:
1251 565 452 327 144
HpaI GTT'AAC
Cuts at: 0 1251 2030 2739
Size: 1251 779 709
Fragments arranged by size:
1251 779 709
MfeI C'AATT_G
Cuts at: 0 1037 1085 2558 2739
Size: 1037 48 1473 181
Fragments arranged by size:
1473 1037 181 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1739 1830 2739
Size: 1739 91 909
Fragments arranged by size:
1739 909 91
MspA1I CmG'CkG
Cuts at: 0 91 777 1195 1678 2739
Size: 91 686 418 483 1061
Fragments arranged by size:
1061 686 483 418 91
NcoI C'CATG_G
Cuts at: 0 1541 2739
Size: 1541 1198
NdeI CA'TA_TG
Cuts at: 0 2067 2739
Size: 2067 672
NspI r_CATG'y
Cuts at: 0 1122 2739
Size: 1122 1617
PasI CC'CwG_GG
Cuts at: 0 269 2739
Size: 269 2470
PfoI T'CCnGG_A
Cuts at: 0 2013 2739
Size: 2013 726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1448 1474 1480 2739
Size: 1442 6 26 6 1259
Fragments arranged by size:
1442 1259 26 6 6
PsiI TTA'TAA
Cuts at: 0 1009 2739
Size: 1009 1730
PstI C_TGCA'G
Cuts at: 0 2348 2739
Size: 2348 391
PvuII CAG'CTG
Cuts at: 0 91 777 1195 1678 2739
Size: 91 686 418 483 1061
Fragments arranged by size:
1061 686 483 418 91
SapI GCTCTTCn'nnn_
Cuts at: 0 1441 2739
Size: 1441 1298
ScaI AGT'ACT
Cuts at: 0 690 2739
Size: 690 2049
SfcI C'TryA_G
Cuts at: 0 728 1328 1733 1865 2344 2513 2739
Size: 728 600 405 132 479 169 226
Fragments arranged by size:
728 600 479 405 226 169 132
SmlI C'TyrA_G
Cuts at: 0 1747 2656 2739
Size: 1747 909 83
Fragments arranged by size:
1747 909 83
SphI G_CATG'C
Cuts at: 0 1122 2739
Size: 1122 1617
SspI AAT'ATT
Cuts at: 0 2022 2739
Size: 2022 717
StuI AGG'CCT
Cuts at: 0 1813 2739
Size: 1813 926
StyI C'CwwG_G
Cuts at: 0 1541 2136 2739
Size: 1541 595 603
Fragments arranged by size:
1541 603 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1321 2739
Size: 1321 1418
TatI w'GTAC_w
Cuts at: 0 688 701 767 2618 2723 2739
Size: 688 13 66 1851 105 16
Fragments arranged by size:
1851 688 105 66 16 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2174 2206 2739
Size: 2174 32 533
Fragments arranged by size:
2174 533 32
XbaI T'CTAG_A
Cuts at: 0 1260 2739
Size: 1260 1479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 2739
Size: 975 1764
XmnI GAAnn'nnTTC
Cuts at: 0 677 2739
Size: 677 2062
Enzymes that do cut:
AclI AcuI AflII AloI AlwNI ApoI AseI AvrII
BanII BbsI BbvCI BcgI BclI BglII BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BsgI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI
BstBI BstYI BtgI BtsI CspCI DraI DraIII EaeI
EarI EciI Eco57MI EcoRI EcoRV HaeII Hin4I HincII
HpaI MfeI MmeI MspA1I NcoI NdeI NspI PasI
PfoI PpiI PsiI PstI PvuII SapI ScaI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI BaeI
BamHI BanI BbeI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmtI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BspEI BspMI BsrBI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DrdI EagI EcoICRI EcoNI
EcoO109I FalI FseI FspI FspAI HindIII KasI KpnI
MluI MscI MslI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI
ZraI