Restriction map for
pFN21AE4586
(Linear) (Six-base) MAPSORT of: KIEE4586.seq Check: 8557 from: 1 to: 2670
KIEE4586.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 570 2670
Size: 570 2100
AatII G_ACGT'C
Cuts at: 0 2393 2670
Size: 2393 277
AccI GT'mk_AC
Cuts at: 0 1526 1976 2670
Size: 1526 450 694
Fragments arranged by size:
1526 694 450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1462 2670
Size: 877 585 1208
Fragments arranged by size:
1208 877 585
AflII C'TTAA_G
Cuts at: 0 2269 2670
Size: 2269 401
AleI CACnn'nnGTG
Cuts at: 0 376 2670
Size: 376 2294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2024 2626 2670
Size: 2024 602 44
Fragments arranged by size:
2024 602 44
ApaI G_GGCC'C
Cuts at: 0 28 1374 2670
Size: 28 1346 1296
Fragments arranged by size:
1346 1296 28
ApoI r'AATT_y
Cuts at: 0 1566 2670
Size: 1566 1104
AvaI C'yCGr_G
Cuts at: 0 122 2670
Size: 122 2548
BanI G'GyrC_C
Cuts at: 0 226 1576 2617 2670
Size: 226 1350 1041 53
Fragments arranged by size:
1350 1041 226 53
BanII G_rGCy'C
Cuts at: 0 28 835 1157 1211 1358 1374 1628 1781
Size: 28 807 322 54 147 16 254 153
Cuts at: 1781 1891 2261 2525 2670
Size: 110 370 264 145
Fragments arranged by size:
807 370 322 264 254 153 147 145
110 54 28 16
BbeI G_GCGC'C
Cuts at: 0 2621 2670
Size: 2621 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 1227 2508 2670
Size: 1227 1281 162
Fragments arranged by size:
1281 1227 162
BbvCI CC'TCA_GC
Cuts at: 0 1561 2670
Size: 1561 1109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 394 428 2448 2482 2670
Size: 394 34 2020 34 188
Fragments arranged by size:
2020 394 188 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 925 1729 2204 2498 2670
Size: 925 804 475 294 172
Fragments arranged by size:
925 804 475 294 172
BlpI GC'TnA_GC
Cuts at: 0 2443 2670
Size: 2443 227
Bme1580I G_kGCm'C
Cuts at: 0 28 659 1374 1579 1598 1820 2670
Size: 28 631 715 205 19 222 850
Fragments arranged by size:
850 715 631 222 205 28 19
BmgBI CAC'GTC
Cuts at: 0 467 1769 2670
Size: 467 1302 901
Fragments arranged by size:
1302 901 467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 535 622 709 906 1225 1666 1795
Size: 295 240 87 87 197 319 441 129
Cuts at: 1795 2098 2506 2670
Size: 303 408 164
Fragments arranged by size:
441 408 319 303 295 240 197 164
129 87 87
Bpu10I CC'TnA_GC
Cuts at: 0 1333 1339 1561 2670
Size: 1333 6 222 1109
Fragments arranged by size:
1333 1109 222 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 2509 2670
Size: 1069 1440 161
Fragments arranged by size:
1440 1069 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 2032 2670
Size: 499 1533 638
Fragments arranged by size:
1533 638 499
BsaAI yAC'GTr
Cuts at: 0 2096 2580 2670
Size: 2096 484 90
Fragments arranged by size:
2096 484 90
BsaHI Gr'CG_yC
Cuts at: 0 770 2390 2618 2670
Size: 770 1620 228 52
Fragments arranged by size:
1620 770 228 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 904 934 2670
Size: 904 30 1736
Fragments arranged by size:
1736 904 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 489 2266 2269 2395 2670
Size: 123 366 1777 3 126 275
Fragments arranged by size:
1777 366 275 126 123 3
BseYI C'CCAG_C
Cuts at: 0 21 194 778 2555 2670
Size: 21 173 584 1777 115
Fragments arranged by size:
1777 584 173 115 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 862 1062 2670
Size: 354 508 200 1608
Fragments arranged by size:
1608 508 354 200
BsiEI CG_ry'CG
Cuts at: 0 2106 2249 2313 2670
Size: 2106 143 64 357
Fragments arranged by size:
2106 357 143 64
BsiHKAI G_wGCw'C
Cuts at: 0 129 629 1345 1628 1781 1891 2261 2525
Size: 129 500 716 283 153 110 370 264
Cuts at: 2525 2594 2670
Size: 69 76
Fragments arranged by size:
716 500 370 283 264 153 129 110
76 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 158 1775 2670
Size: 158 1617 895
Fragments arranged by size:
1617 895 158
Bsp1286I G_dGCh'C
Cuts at: 0 28 129 629 659 835 1157 1211 1345
Size: 28 101 500 30 176 322 54 134
Cuts at: 1345 1358 1374 1579 1598 1628 1781 1820 1891
Size: 13 16 205 19 30 153 39 71
Cuts at: 1891 2261 2525 2594 2670
Size: 370 264 69 76
Fragments arranged by size:
500 370 322 264 205 176 153 134
101 76 71 69 54 39 30 30
28 19 16 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 570 851 1145 1538 2670
Size: 338 232 281 294 393 1132
Fragments arranged by size:
1132 393 338 294 281 232
BsrBI CCG'CTC
Cuts at: 0 1958 2670
Size: 1958 712
BsrFI r'CCGG_y
Cuts at: 0 340 437 1306 1393 1654 1723 2101 2670
Size: 340 97 869 87 261 69 378 569
Fragments arranged by size:
869 569 378 340 261 97 87 69
BssHII G'CGCG_C
Cuts at: 0 2050 2670
Size: 2050 620
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1274 1283 1583 2670
Size: 330 944 9 300 1087
Fragments arranged by size:
1087 944 330 300 9
BstEII G'GTnAC_C
Cuts at: 0 1386 2670
Size: 1386 1284
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1659 1719 2030 2670
Size: 1659 60 311 640
Fragments arranged by size:
1659 640 311 60
BstYI r'GATC_y
Cuts at: 0 2060 2670
Size: 2060 610
Bsu36I CC'TnA_GG
Cuts at: 0 836 2670
Size: 836 1834
BtgI C'CryG_G
Cuts at: 0 587 1067 1631 1799 1892 2243 2333 2670
Size: 587 480 564 168 93 351 90 337
Fragments arranged by size:
587 564 480 351 337 168 93 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 708 1245 2670
Size: 708 537 1425
Fragments arranged by size:
1425 708 537
BtsI GCAGTG_nn'
Cuts at: 0 11 847 2670
Size: 11 836 1823
Fragments arranged by size:
1823 836 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 388 2670
Size: 388 2282
EaeI y'GGCC_r
Cuts at: 0 338 584 612 1376 1452 1479 1721 1922
Size: 338 246 28 764 76 27 242 201
Cuts at: 1922 2103 2246 2279 2670
Size: 181 143 33 391
Fragments arranged by size:
764 391 338 246 242 201 181 143
76 33 28 27
EagI C'GGCC_G
Cuts at: 0 2103 2246 2670
Size: 2103 143 424
Fragments arranged by size:
2103 424 143
EarI CTCTTCn'nnn_
Cuts at: 0 450 823 1546 1699 2670
Size: 450 373 723 153 971
Fragments arranged by size:
971 723 450 373 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 607 2670
Size: 463 144 2063
Fragments arranged by size:
2063 463 144
EcoICRI GAG'CTC
Cuts at: 0 1626 1779 1889 2259 2523 2670
Size: 1626 153 110 370 264 147
Fragments arranged by size:
1626 370 264 153 147 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 535 622 709 877 906 1225 1462
Size: 295 240 87 87 168 29 319 237
Cuts at: 1462 1666 1795 2098 2506 2670
Size: 204 129 303 408 164
Fragments arranged by size:
408 319 303 295 240 237 204 168
164 129 87 87 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2452 2670
Size: 2452 218
EcoO109I rG'GnC_Cy
Cuts at: 0 25 119 294 649 840 1370 1371 1446
Size: 25 94 175 355 191 530 1 75
Cuts at: 1446 2670
Size: 1224
Fragments arranged by size:
1224 530 355 191 175 94 75 25
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1866 2649 2670
Size: 1866 783 21
Fragments arranged by size:
1866 783 21
FseI GG_CCGG'CC
Cuts at: 0 1727 2670
Size: 1727 943
FspI TGC'GCA
Cuts at: 0 529 2670
Size: 529 2141
HaeII r_GCGC'y
Cuts at: 0 696 2147 2360 2621 2670
Size: 696 1451 213 261 49
Fragments arranged by size:
1451 696 261 213 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 2670
Size: 904 32 1734
Fragments arranged by size:
1734 904 32
HincII GTy'rAC
Cuts at: 0 621 1185 2670
Size: 621 564 1485
Fragments arranged by size:
1485 621 564
KasI G'GCGC_C
Cuts at: 0 2617 2670
Size: 2617 53
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 77 2670
Size: 32 45 2593
Fragments arranged by size:
2593 45 32
MscI TGG'CCA
Cuts at: 0 1378 1454 2281 2670
Size: 1378 76 827 389
Fragments arranged by size:
1378 827 389 76
MslI CAynn'nnrTG
Cuts at: 0 376 1066 1468 1717 1909 2161 2670
Size: 376 690 402 249 192 252 509
Fragments arranged by size:
690 509 402 376 252 249 192
MspA1I CmG'CkG
Cuts at: 0 21 172 181 368 376 1038 1560 2354
Size: 21 151 9 187 8 662 522 794
Cuts at: 2354 2537 2670
Size: 183 133
Fragments arranged by size:
794 662 522 187 183 151 133 21
9 8
NaeI GCC'GGC
Cuts at: 0 342 1395 1725 2670
Size: 342 1053 330 945
Fragments arranged by size:
1053 945 342 330
NarI GG'CG_CC
Cuts at: 0 2618 2670
Size: 2618 52
NcoI C'CATG_G
Cuts at: 0 1067 1892 2670
Size: 1067 825 778
Fragments arranged by size:
1067 825 778
NgoMIV G'CCGG_C
Cuts at: 0 340 1393 1723 2670
Size: 340 1053 330 947
Fragments arranged by size:
1053 947 340 330
NruI TCG'CGA
Cuts at: 0 416 2670
Size: 416 2254
NspI r_CATG'y
Cuts at: 0 687 936 2166 2343 2670
Size: 687 249 1230 177 327
Fragments arranged by size:
1230 687 327 249 177
PfoI T'CCnGG_A
Cuts at: 0 272 1094 2483 2670
Size: 272 822 1389 187
Fragments arranged by size:
1389 822 272 187
PmlI CAC'GTG
Cuts at: 0 2096 2580 2670
Size: 2096 484 90
Fragments arranged by size:
2096 484 90
PpuMI rG'GwC_Cy
Cuts at: 0 294 840 1446 2670
Size: 294 546 606 1224
Fragments arranged by size:
1224 606 546 294
PspOMI G'GGCC_C
Cuts at: 0 24 1370 2670
Size: 24 1346 1300
Fragments arranged by size:
1346 1300 24
PstI C_TGCA'G
Cuts at: 0 335 671 797 1751 2071 2227 2670
Size: 335 336 126 954 320 156 443
Fragments arranged by size:
954 443 336 335 320 156 126
PvuII CAG'CTG
Cuts at: 0 368 1560 2537 2670
Size: 368 1192 977 133
Fragments arranged by size:
1192 977 368 133
SacI G_AGCT'C
Cuts at: 0 1628 1781 1891 2261 2525 2670
Size: 1628 153 110 370 264 145
Fragments arranged by size:
1628 370 264 153 145 110
SanDI GG'GwC_CC
Cuts at: 0 294 2670
Size: 294 2376
SapI GCTCTTCn'nnn_
Cuts at: 0 823 2670
Size: 823 1847
SbfI CC_TGCA'GG
Cuts at: 0 797 2670
Size: 797 1873
SexAI A'CCwGG_T
Cuts at: 0 1484 1601 2670
Size: 1484 117 1069
Fragments arranged by size:
1484 1069 117
SfcI C'TryA_G
Cuts at: 0 331 667 793 1747 1809 2067 2223 2670
Size: 331 336 126 954 62 258 156 447
Fragments arranged by size:
954 447 336 331 258 156 126 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1729 2670
Size: 1729 941
SfoI GGC'GCC
Cuts at: 0 2619 2670
Size: 2619 51
SgrAI Cr'CCGG_yG
Cuts at: 0 437 2670
Size: 437 2233
SmlI C'TyrA_G
Cuts at: 0 1084 2269 2524 2670
Size: 1084 1185 255 146
Fragments arranged by size:
1185 1084 255 146
SphI G_CATG'C
Cuts at: 0 687 936 2343 2670
Size: 687 249 1407 327
Fragments arranged by size:
1407 687 327 249
StuI AGG'CCT
Cuts at: 0 1108 2212 2670
Size: 1108 1104 458
Fragments arranged by size:
1108 1104 458
StyI C'CwwG_G
Cuts at: 0 284 299 764 1067 1449 1841 1892 2396
Size: 284 15 465 303 382 392 51 504
Cuts at: 2396 2670
Size: 274
Fragments arranged by size:
504 465 392 382 303 284 274 51
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 523 1360 2071 2580 2670
Size: 523 837 711 509 90
Fragments arranged by size:
837 711 523 509 90
TatI w'GTAC_w
Cuts at: 0 548 2670
Size: 548 2122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 928 960 2670
Size: 928 32 1710
Fragments arranged by size:
1710 928 32
Tth111I GACn'n_nGTC
Cuts at: 0 2389 2670
Size: 2389 281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 145 1164 1854 2670
Size: 93 52 1019 690 816
Fragments arranged by size:
1019 816 690 93 52
ZraI GAC'GTC
Cuts at: 0 2391 2670
Size: 2391 279
Enzymes that do cut:
AarI AatII AccI AcuI AflII AleI AlfI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AhdI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BmrI BmtI BplI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NsiI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI
SgfI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI XmnI