Restriction map for
pFN21AE4583
(Linear) (Six-base) MAPSORT of: KIEE4583.seq Check: 8119 from: 1 to: 2520
KIEE4583.orf
With 182 enzymes: SgfI *
March 22, 2012 18:25 ..
AccI GT'mk_AC
Cuts at: 0 1460 2520
Size: 1460 1060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 2109 2402 2520
Size: 103 2006 293 118
Fragments arranged by size:
2006 293 118 103
AflIII A'CryG_T
Cuts at: 0 84 2024 2520
Size: 84 1940 496
Fragments arranged by size:
1940 496 84
AleI CACnn'nnGTG
Cuts at: 0 2171 2520
Size: 2171 349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 2520
Size: 404 32 2084
Fragments arranged by size:
2084 404 32
AlwNI CAG_nnn'CTG
Cuts at: 0 824 2109 2520
Size: 824 1285 411
Fragments arranged by size:
1285 824 411
ApoI r'AATT_y
Cuts at: 0 235 514 572 918 993 2520
Size: 235 279 58 346 75 1527
Fragments arranged by size:
1527 346 279 235 75 58
BamHI G'GATC_C
Cuts at: 0 1316 2520
Size: 1316 1204
BanI G'GyrC_C
Cuts at: 0 1447 2520
Size: 1447 1073
BanII G_rGCy'C
Cuts at: 0 108 889 1413 1426 1798 2010 2407 2478
Size: 108 781 524 13 372 212 397 71
Cuts at: 2478 2520
Size: 42
Fragments arranged by size:
781 524 397 372 212 108 71 42
13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1481 1730 2267 2520
Size: 1481 249 537 253
Fragments arranged by size:
1481 537 253 249
BbvCI CC'TCA_GC
Cuts at: 0 1158 2520
Size: 1158 1362
BclI T'GATC_A
Cuts at: 0 32 2520
Size: 32 2488
BglI GCCn_nnn'nGGC
Cuts at: 0 1223 2520
Size: 1223 1297
BglII A'GATC_T
Cuts at: 0 561 2520
Size: 561 1959
BlpI GC'TnA_GC
Cuts at: 0 2116 2520
Size: 2116 404
Bme1580I G_kGCm'C
Cuts at: 0 1184 2493 2520
Size: 1184 1309 27
Fragments arranged by size:
1309 1184 27
BmgBI CAC'GTC
Cuts at: 0 1092 2025 2499 2520
Size: 1092 933 474 21
Fragments arranged by size:
1092 933 474 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 577 1948 2520
Size: 577 1371 572
Fragments arranged by size:
1371 577 572
BmtI G_CTAG'C
Cuts at: 0 670 2520
Size: 670 1850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2497 2520
Size: 2497 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1080 1937 2520
Size: 130 950 857 583
Fragments arranged by size:
950 857 583 130
Bpu10I CC'TnA_GC
Cuts at: 0 1104 1158 1666 2520
Size: 1104 54 508 854
Fragments arranged by size:
1104 854 508 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2190 2376 2520
Size: 2190 186 144
Fragments arranged by size:
2190 186 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 2126 2287 2520
Size: 2126 161 233
Fragments arranged by size:
2126 233 161
BsaAI yAC'GTr
Cuts at: 0 87 1965 2015 2520
Size: 87 1878 50 505
Fragments arranged by size:
1878 505 87 50
BsaBI GATnn'nnATC
Cuts at: 0 176 2520
Size: 176 2344
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 482 512 2520
Size: 482 30 2008
Fragments arranged by size:
2008 482 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 506 989 2520
Size: 506 483 1531
Fragments arranged by size:
1531 506 483
BseYI C'CCAG_C
Cuts at: 0 1145 1660 1821 2520
Size: 1145 515 161 699
Fragments arranged by size:
1145 699 515 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2149 2520
Size: 2149 371
BsiEI CG_ry'CG
Cuts at: 0 2268 2520
Size: 2268 252
BsiHKAI G_wGCw'C
Cuts at: 0 108 259 1413 2407 2520
Size: 108 151 1154 994 113
Fragments arranged by size:
1154 994 151 113 108
BsiWI C'GTAC_G
Cuts at: 0 2015 2520
Size: 2015 505
BsmI GAATG_Cn'
Cuts at: 0 1815 2038 2520
Size: 1815 223 482
Fragments arranged by size:
1815 482 223
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 2520
Size: 1098 1422
Bsp1286I G_dGCh'C
Cuts at: 0 108 259 889 1184 1413 1426 1798 2010
Size: 108 151 630 295 229 13 372 212
Cuts at: 2010 2407 2478 2493 2520
Size: 397 71 15 27
Fragments arranged by size:
630 397 372 295 229 212 151 108
71 27 15 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 839 2520
Size: 839 1681
BsrDI GCAATG_nn'
Cuts at: 0 851 1157 1269 1934 2447 2520
Size: 851 306 112 665 513 73
Fragments arranged by size:
851 665 513 306 112 73
BssSI C'ACGA_G
Cuts at: 0 1192 2520
Size: 1192 1328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2109 2520
Size: 2109 411
BstEII G'GTnAC_C
Cuts at: 0 1186 2520
Size: 1186 1334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1821 2520
Size: 1821 699
BstYI r'GATC_y
Cuts at: 0 561 1316 2520
Size: 561 755 1204
Fragments arranged by size:
1204 755 561
Bsu36I CC'TnA_GG
Cuts at: 0 424 1338 2221 2520
Size: 424 914 883 299
Fragments arranged by size:
914 883 424 299
BtgI C'CryG_G
Cuts at: 0 431 1403 2520
Size: 431 972 1117
Fragments arranged by size:
1117 972 431
DraIII CAC_nnn'GTG
Cuts at: 0 70 1615 2520
Size: 70 1545 905
Fragments arranged by size:
1545 905 70
EaeI y'GGCC_r
Cuts at: 0 864 1224 1400 1893 1959 2265 2520
Size: 864 360 176 493 66 306 255
Fragments arranged by size:
864 493 360 306 255 176 66
EagI C'GGCC_G
Cuts at: 0 2265 2520
Size: 2265 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2081 2520
Size: 2081 439
EcoICRI GAG'CTC
Cuts at: 0 106 1411 2405 2520
Size: 106 1305 994 115
Fragments arranged by size:
1305 994 115 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 130 1080 1937 2109 2402 2520
Size: 103 27 950 857 172 293 118
Fragments arranged by size:
950 857 293 172 118 103 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1171 1300 2520
Size: 1171 129 1220
Fragments arranged by size:
1220 1171 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 439 2483 2520
Size: 439 2044 37
Fragments arranged by size:
2044 439 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 2011 2043 2224 2256 2520
Size: 1000 32 979 32 181 32 264
Fragments arranged by size:
1000 979 264 181 32 32 32
HincII GTy'rAC
Cuts at: 0 141 948 1287 2520
Size: 141 807 339 1233
Fragments arranged by size:
1233 807 339 141
HindIII A'AGCT_T
Cuts at: 0 795 2520
Size: 795 1725
HpaI GTT'AAC
Cuts at: 0 141 2520
Size: 141 2379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1349 1983 2520
Size: 785 564 634 537
Fragments arranged by size:
785 634 564 537
MscI TGG'CCA
Cuts at: 0 1961 2520
Size: 1961 559
MslI CAynn'nnrTG
Cuts at: 0 170 2077 2171 2416 2520
Size: 170 1907 94 245 104
Fragments arranged by size:
1907 245 170 104 94
MspA1I CmG'CkG
Cuts at: 0 392 1162 2129 2520
Size: 392 770 967 391
Fragments arranged by size:
967 770 392 391
NcoI C'CATG_G
Cuts at: 0 431 2520
Size: 431 2089
NheI G'CTAG_C
Cuts at: 0 666 2520
Size: 666 1854
NspI r_CATG'y
Cuts at: 0 860 2082 2520
Size: 860 1222 438
Fragments arranged by size:
1222 860 438
PasI CC'CwG_GG
Cuts at: 0 320 882 2520
Size: 320 562 1638
Fragments arranged by size:
1638 562 320
PfoI T'CCnGG_A
Cuts at: 0 997 2520
Size: 997 1523
PstI C_TGCA'G
Cuts at: 0 1560 2520
Size: 1560 960
PvuII CAG'CTG
Cuts at: 0 392 1162 2520
Size: 392 770 1358
Fragments arranged by size:
1358 770 392
SacI G_AGCT'C
Cuts at: 0 108 1413 2407 2520
Size: 108 1305 994 113
Fragments arranged by size:
1305 994 113 108
SexAI A'CCwGG_T
Cuts at: 0 1611 2520
Size: 1611 909
SfcI C'TryA_G
Cuts at: 0 144 1212 1556 2502 2520
Size: 144 1068 344 946 18
Fragments arranged by size:
1068 946 344 144 18
SmlI C'TyrA_G
Cuts at: 0 2169 2391 2520
Size: 2169 222 129
Fragments arranged by size:
2169 222 129
SnaBI TAC'GTA
Cuts at: 0 2015 2520
Size: 2015 505
SspI AAT'ATT
Cuts at: 0 780 2520
Size: 780 1740
StyI C'CwwG_G
Cuts at: 0 431 1358 1860 2061 2301 2426 2508 2520
Size: 431 927 502 201 240 125 82 12
Fragments arranged by size:
927 502 431 240 201 125 82 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 2520
Size: 895 1625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 2520
Size: 482 32 2006
Fragments arranged by size:
2006 482 32
XmnI GAAnn'nnTTC
Cuts at: 0 366 1488 2463 2520
Size: 366 1122 975 57
Fragments arranged by size:
1122 975 366 57
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI BamHI
BanI BanII BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI FalI Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NcoI NheI
NspI PasI PfoI PstI PvuII SacI SexAI SfcI
SmlI SnaBI SspI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BfrBI BsaHI BsaWI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EarI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI