Restriction map for
pFN21AE4578
(Linear) (Six-base) MAPSORT of: KIEE4578.seq Check: 2305 from: 1 to: 2322
KIEE4578.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 877 2322
Size: 877 1445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 2238 2271 2322
Size: 903 1335 33 51
Fragments arranged by size:
1335 903 51 33
AfeI AGC'GCT
Cuts at: 0 769 2322
Size: 769 1553
AflIII A'CryG_T
Cuts at: 0 1853 2060 2322
Size: 1853 207 262
Fragments arranged by size:
1853 262 207
AhdI GACnn_n'nnGTC
Cuts at: 0 1071 2322
Size: 1071 1251
AleI CACnn'nnGTG
Cuts at: 0 598 1939 2104 2243 2322
Size: 598 1341 165 139 79
Fragments arranged by size:
1341 598 165 139 79
AlwNI CAG_nnn'CTG
Cuts at: 0 1129 1967 2322
Size: 1129 838 355
Fragments arranged by size:
1129 838 355
ApaI G_GGCC'C
Cuts at: 0 359 835 2322
Size: 359 476 1487
Fragments arranged by size:
1487 476 359
ApaLI G'TGCA_C
Cuts at: 0 1182 2322
Size: 1182 1140
ApoI r'AATT_y
Cuts at: 0 499 675 2322
Size: 499 176 1647
Fragments arranged by size:
1647 499 176
AvaI C'yCGr_G
Cuts at: 0 100 936 1533 2322
Size: 100 836 597 789
Fragments arranged by size:
836 789 597 100
BanI G'GyrC_C
Cuts at: 0 241 991 1933 2322
Size: 241 750 942 389
Fragments arranged by size:
942 750 389 241
BanII G_rGCy'C
Cuts at: 0 359 835 2297 2322
Size: 359 476 1462 25
Fragments arranged by size:
1462 476 359 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 909 2322
Size: 909 1413
BbvCI CC'TCA_GC
Cuts at: 0 1746 2322
Size: 1746 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1237 1271 2322
Size: 1237 34 1051
Fragments arranged by size:
1237 1051 34
BclI T'GATC_A
Cuts at: 0 515 2322
Size: 515 1807
BglI GCCn_nnn'nGGC
Cuts at: 0 157 239 1120 1313 1439 1595 2322
Size: 157 82 881 193 126 156 727
Fragments arranged by size:
881 727 193 157 156 126 82
BglII A'GATC_T
Cuts at: 0 647 2322
Size: 647 1675
Bme1580I G_kGCm'C
Cuts at: 0 244 359 835 1186 2111 2322
Size: 244 115 476 351 925 211
Fragments arranged by size:
925 476 351 244 211 115
BmgBI CAC'GTC
Cuts at: 0 282 1581 2106 2322
Size: 282 1299 525 216
Fragments arranged by size:
1299 525 282 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 592 1150 1410 2322
Size: 592 558 260 912
Fragments arranged by size:
912 592 558 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 589 2322
Size: 589 1733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 1708 2135 2322
Size: 801 907 427 187
Fragments arranged by size:
907 801 427 187
Bpu10I CC'TnA_GC
Cuts at: 0 64 1746 2322
Size: 64 1682 576
Fragments arranged by size:
1682 576 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 1465 2322
Size: 1465 857
BsaAI yAC'GTr
Cuts at: 0 306 615 2322
Size: 306 309 1707
Fragments arranged by size:
1707 309 306
BsaHI Gr'CG_yC
Cuts at: 0 431 2322
Size: 431 1891
BsaWI w'CCGG_w
Cuts at: 0 944 2322
Size: 944 1378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 301 331 1462 1492 2322
Size: 247 30 24 30 1131 30 830
Fragments arranged by size:
1131 830 247 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 324 1264 1741 1788 2322
Size: 324 940 477 47 534
Fragments arranged by size:
940 534 477 324 47
BseYI C'CCAG_C
Cuts at: 0 181 297 425 828 1098 1437 2054 2322
Size: 181 116 128 403 270 339 617 268
Fragments arranged by size:
617 403 339 270 268 181 128 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 777 1624 1690 2322
Size: 492 285 847 66 632
Fragments arranged by size:
847 632 492 285 66
BsiEI CG_ry'CG
Cuts at: 0 890 1320 1848 2322
Size: 890 430 528 474
Fragments arranged by size:
890 528 474 430
BsiHKAI G_wGCw'C
Cuts at: 0 19 1025 1186 1534 2285 2322
Size: 19 1006 161 348 751 37
Fragments arranged by size:
1006 751 348 161 37 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2257 2322
Size: 2257 65
Bsp1286I G_dGCh'C
Cuts at: 0 19 244 359 835 1025 1186 1534 2111
Size: 19 225 115 476 190 161 348 577
Cuts at: 2111 2285 2297 2322
Size: 174 12 25
Fragments arranged by size:
577 476 348 225 190 174 161 115
25 19 12
BspEI T'CCGG_A
Cuts at: 0 944 2322
Size: 944 1378
BspHI T'CATG_A
Cuts at: 0 1514 2186 2322
Size: 1514 672 136
Fragments arranged by size:
1514 672 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 877 903 1925 2322
Size: 877 26 1022 397
Fragments arranged by size:
1022 877 397 26
BsrDI GCAATG_nn'
Cuts at: 0 1197 2322
Size: 1197 1125
BsrFI r'CCGG_y
Cuts at: 0 117 796 885 1694 1781 2322
Size: 117 679 89 809 87 541
Fragments arranged by size:
809 679 541 117 89 87
BsrGI T'GTAC_A
Cuts at: 0 737 2228 2322
Size: 737 1491 94
Fragments arranged by size:
1491 737 94
BssSI C'ACGA_G
Cuts at: 0 1246 2322
Size: 1246 1076
BstAPI GCAn_nnn'nTGC
Cuts at: 0 248 2322
Size: 248 2074
BstXI CCAn_nnnn'nTGG
Cuts at: 0 385 1686 1816 2245 2322
Size: 385 1301 130 429 77
Fragments arranged by size:
1301 429 385 130 77
BstYI r'GATC_y
Cuts at: 0 647 1175 1805 1859 2322
Size: 647 528 630 54 463
Fragments arranged by size:
647 630 528 463 54
BtgI C'CryG_G
Cuts at: 0 785 890 1283 1418 1721 1769 2045 2322
Size: 785 105 393 135 303 48 276 277
Fragments arranged by size:
785 393 303 277 276 135 105 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 266 2322
Size: 266 2056
BtsI GCAGTG_nn'
Cuts at: 0 1557 1720 2322
Size: 1557 163 602
Fragments arranged by size:
1557 602 163
DraIII CAC_nnn'GTG
Cuts at: 0 248 2322
Size: 248 2074
EaeI y'GGCC_r
Cuts at: 0 158 231 668 775 887 1076 1317 1392
Size: 158 73 437 107 112 189 241 75
Cuts at: 1392 1818 1845 2211 2322
Size: 426 27 366 111
Fragments arranged by size:
437 426 366 241 189 158 112 111
107 75 73 27
EagI C'GGCC_G
Cuts at: 0 887 1317 1845 2322
Size: 887 430 528 477
Fragments arranged by size:
887 528 477 430
EarI CTCTTCn'nnn_
Cuts at: 0 184 2255 2322
Size: 184 2071 67
Fragments arranged by size:
2071 184 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1613 2322
Size: 1613 709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 903 1708 2135 2238 2271 2322
Size: 801 102 805 427 103 33 51
Fragments arranged by size:
805 801 427 103 102 51 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 756 2128 2322
Size: 756 1372 194
Fragments arranged by size:
1372 756 194
EcoO109I rG'GnC_Cy
Cuts at: 0 144 355 832 1065 1367 2322
Size: 144 211 477 233 302 955
Fragments arranged by size:
955 477 302 233 211 144
EcoRI G'AATT_C
Cuts at: 0 675 2322
Size: 675 1647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1980 2322
Size: 1980 342
HaeII r_GCGC'y
Cuts at: 0 771 2322
Size: 771 1551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 301 333 919 951 1057 1089
Size: 247 32 22 32 586 32 106 32
Cuts at: 1089 2322
Size: 1233
Fragments arranged by size:
1233 586 247 106 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 1497 2094 2322
Size: 1497 597 228
Fragments arranged by size:
1497 597 228
MluI A'CGCG_T
Cuts at: 0 1853 2322
Size: 1853 469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 2201 2322
Size: 1850 351 121
Fragments arranged by size:
1850 351 121
MscI TGG'CCA
Cuts at: 0 160 233 777 1394 1820 2322
Size: 160 73 544 617 426 502
Fragments arranged by size:
617 544 502 426 160 73
MslI CAynn'nnrTG
Cuts at: 0 598 1490 1814 1897 1939 2044 2104 2224
Size: 598 892 324 83 42 105 60 120
Cuts at: 2224 2243 2322
Size: 19 79
Fragments arranged by size:
892 598 324 120 105 83 79 60
42 19
MspA1I CmG'CkG
Cuts at: 0 32 86 171 821 1437 2291 2310 2322
Size: 32 54 85 650 616 854 19 12
Fragments arranged by size:
854 650 616 85 54 32 19 12
NaeI GCC'GGC
Cuts at: 0 1783 2322
Size: 1783 539
NcoI C'CATG_G
Cuts at: 0 785 1283 1418 1721 2045 2322
Size: 785 498 135 303 324 277
Fragments arranged by size:
785 498 324 303 277 135
NgoMIV G'CCGG_C
Cuts at: 0 1781 2322
Size: 1781 541
NotI GC'GGCC_GC
Cuts at: 0 1317 2322
Size: 1317 1005
NspI r_CATG'y
Cuts at: 0 413 699 2064 2322
Size: 413 286 1365 258
Fragments arranged by size:
1365 413 286 258
PasI CC'CwG_GG
Cuts at: 0 709 1593 2322
Size: 709 884 729
Fragments arranged by size:
884 729 709
PciI A'CATG_T
Cuts at: 0 2060 2322
Size: 2060 262
PflMI CCAn_nnn'nTGG
Cuts at: 0 320 425 1893 2322
Size: 320 105 1468 429
Fragments arranged by size:
1468 429 320 105
PfoI T'CCnGG_A
Cuts at: 0 319 1466 2322
Size: 319 1147 856
Fragments arranged by size:
1147 856 319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1723 1755 2322
Size: 1723 32 567
Fragments arranged by size:
1723 567 32
PpuMI rG'GwC_Cy
Cuts at: 0 1065 1367 2322
Size: 1065 302 955
Fragments arranged by size:
1065 955 302
PspOMI G'GGCC_C
Cuts at: 0 355 831 2322
Size: 355 476 1491
Fragments arranged by size:
1491 476 355
PstI C_TGCA'G
Cuts at: 0 31 1093 1715 2322
Size: 31 1062 622 607
Fragments arranged by size:
1062 622 607 31
PvuII CAG'CTG
Cuts at: 0 32 86 171 821 1437 2310 2322
Size: 32 54 85 650 616 873 12
Fragments arranged by size:
873 650 616 85 54 32 12
SanDI GG'GwC_CC
Cuts at: 0 1367 2322
Size: 1367 955
ScaI AGT'ACT
Cuts at: 0 106 2322
Size: 106 2216
SexAI A'CCwGG_T
Cuts at: 0 1067 1474 1646 2322
Size: 1067 407 172 676
Fragments arranged by size:
1067 676 407 172
SfcI C'TryA_G
Cuts at: 0 27 1089 1711 2322
Size: 27 1062 622 611
Fragments arranged by size:
1062 622 611 27
SmlI C'TyrA_G
Cuts at: 0 936 2322
Size: 936 1386
SphI G_CATG'C
Cuts at: 0 413 2322
Size: 413 1909
StuI AGG'CCT
Cuts at: 0 9 2322
Size: 9 2313
StyI C'CwwG_G
Cuts at: 0 785 1283 1418 1721 1937 2045 2322
Size: 785 498 135 303 216 108 277
Fragments arranged by size:
785 498 303 277 216 135 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1774 1921 2322
Size: 1774 147 401
Fragments arranged by size:
1774 401 147
TatI w'GTAC_w
Cuts at: 0 104 221 382 737 2228 2322
Size: 104 117 161 355 1491 94
Fragments arranged by size:
1491 355 161 117 104 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1374 1406 2322
Size: 1374 32 916
Fragments arranged by size:
1374 916 32
Tth111I GACn'n_nGTC
Cuts at: 0 1648 2322
Size: 1648 674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 825 1968 2322
Size: 825 1143 354
Fragments arranged by size:
1143 825 354
XhoI C'TCGA_G
Cuts at: 0 936 2322
Size: 936 1386
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI
NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SanDI ScaI SexAI SfcI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BfrBI BlpI BmtI BpuEI BsaBI BsiWI BsmI BsrBI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI NarI NdeI NheI NruI NsiI
PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
XbaI XmaI XmnI ZraI