Restriction map for
pFN21AE4577
(Linear) (Six-base) MAPSORT of: KIEE4577.seq Check: 2695 from: 1 to: 2292
KIEE4577.orf
With 182 enzymes: SgfI *
December 3, 2014 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2041 2173 2292
Size: 2041 132 119
Fragments arranged by size:
2041 132 119
Acc65I G'GTAC_C
Cuts at: 0 1481 2292
Size: 1481 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1264 2094 2292
Size: 913 351 830 198
Fragments arranged by size:
913 830 351 198
AfeI AGC'GCT
Cuts at: 0 495 2101 2292
Size: 495 1606 191
Fragments arranged by size:
1606 495 191
AflII C'TTAA_G
Cuts at: 0 379 2292
Size: 379 1913
AflIII A'CryG_T
Cuts at: 0 1202 1373 1540 2292
Size: 1202 171 167 752
Fragments arranged by size:
1202 752 171 167
AleI CACnn'nnGTG
Cuts at: 0 1444 2292
Size: 1444 848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1882 2292
Size: 1882 410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 2292
Size: 109 32 2151
Fragments arranged by size:
2151 109 32
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1434 1458 2151 2292
Size: 450 984 24 693 141
Fragments arranged by size:
984 693 450 141 24
ApaI G_GGCC'C
Cuts at: 0 834 1764 2292
Size: 834 930 528
Fragments arranged by size:
930 834 528
ApaLI G'TGCA_C
Cuts at: 0 465 2292
Size: 465 1827
ApoI r'AATT_y
Cuts at: 0 145 1804 2292
Size: 145 1659 488
Fragments arranged by size:
1659 488 145
AvaI C'yCGr_G
Cuts at: 0 251 262 572 2200 2292
Size: 251 11 310 1628 92
Fragments arranged by size:
1628 310 251 92 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1758 1791 2292
Size: 1758 33 501
Fragments arranged by size:
1758 501 33
BamHI G'GATC_C
Cuts at: 0 207 2292
Size: 207 2085
BanI G'GyrC_C
Cuts at: 0 193 1367 1481 1682 2245 2292
Size: 193 1174 114 201 563 47
Fragments arranged by size:
1174 563 201 193 114 47
BanII G_rGCy'C
Cuts at: 0 340 818 834 1093 1124 1764 1799 2292
Size: 340 478 16 259 31 640 35 493
Fragments arranged by size:
640 493 478 340 259 35 31 16
BbeI G_GCGC'C
Cuts at: 0 1371 1686 2292
Size: 1371 315 606
Fragments arranged by size:
1371 606 315
BbsI GAAGACnn'nnnn_
Cuts at: 0 1623 2292
Size: 1623 669
BbvCI CC'TCA_GC
Cuts at: 0 443 2292
Size: 443 1849
BciVI GTATCCnnnnn_n'
Cuts at: 0 1786 2292
Size: 1786 506
Bme1580I G_kGCm'C
Cuts at: 0 469 834 1764 2270 2292
Size: 469 365 930 506 22
Fragments arranged by size:
930 506 469 365 22
BmgBI CAC'GTC
Cuts at: 0 353 2292
Size: 353 1939
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 1758 2139 2292
Size: 456 1302 381 153
Fragments arranged by size:
1302 456 381 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 187 250 1071 2292
Size: 187 63 821 1221
Fragments arranged by size:
1221 821 187 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 706 1084 1989 2026 2266 2292
Size: 5 701 378 905 37 240 26
Fragments arranged by size:
905 701 378 240 37 26 5
Bpu10I CC'TnA_GC
Cuts at: 0 292 443 1666 2292
Size: 292 151 1223 626
Fragments arranged by size:
1223 626 292 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 2292
Size: 877 1415
BsaI GGTCTCn'nnnn_
Cuts at: 0 252 1933 2292
Size: 252 1681 359
Fragments arranged by size:
1681 359 252
BsaAI yAC'GTr
Cuts at: 0 1374 1543 1887 2292
Size: 1374 169 344 405
Fragments arranged by size:
1374 405 344 169
BsaHI Gr'CG_yC
Cuts at: 0 1368 1683 2292
Size: 1368 315 609
Fragments arranged by size:
1368 609 315
BsaWI w'CCGG_w
Cuts at: 0 140 2292
Size: 140 2152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 2292
Size: 109 30 2153
Fragments arranged by size:
2153 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 534 808 1063 1327 2134 2269 2292
Size: 534 274 255 264 807 135 23
Fragments arranged by size:
807 534 274 264 255 135 23
BseYI C'CCAG_C
Cuts at: 0 826 1512 2292
Size: 826 686 780
Fragments arranged by size:
826 780 686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 871 1744 1831 2052 2292
Size: 484 387 873 87 221 240
Fragments arranged by size:
873 484 387 240 221 87
BsiEI CG_ry'CG
Cuts at: 0 585 2292
Size: 585 1707
BsiHKAI G_wGCw'C
Cuts at: 0 469 1799 2292
Size: 469 1330 493
Fragments arranged by size:
1330 493 469
BsmI GAATG_Cn'
Cuts at: 0 401 2292
Size: 401 1891
Bsp1286I G_dGCh'C
Cuts at: 0 340 469 818 834 1093 1124 1764 1799
Size: 340 129 349 16 259 31 640 35
Cuts at: 1799 2270 2292
Size: 471 22
Fragments arranged by size:
640 471 349 340 259 129 35 31
22 16
BspHI T'CATG_A
Cuts at: 0 1574 1931 2292
Size: 1574 357 361
Fragments arranged by size:
1574 361 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 881 1493 1535 1610 2041 2173 2292
Size: 881 612 42 75 431 132 119
Fragments arranged by size:
881 612 431 132 119 75 42
BsrBI CCG'CTC
Cuts at: 0 101 2292
Size: 101 2191
BsrDI GCAATG_nn'
Cuts at: 0 785 1070 1080 2292
Size: 785 285 10 1212
Fragments arranged by size:
1212 785 285 10
BssSI C'ACGA_G
Cuts at: 0 1339 2292
Size: 1339 953
BstAPI GCAn_nnn'nTGC
Cuts at: 0 939 2151 2292
Size: 939 1212 141
Fragments arranged by size:
1212 939 141
BstEII G'GTnAC_C
Cuts at: 0 795 2292
Size: 795 1497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 61 1818 2292
Size: 61 1757 474
Fragments arranged by size:
1757 474 61
BstYI r'GATC_y
Cuts at: 0 207 267 879 1152 1328 1385 2292
Size: 207 60 612 273 176 57 907
Fragments arranged by size:
907 612 273 207 176 60 57
BtgI C'CryG_G
Cuts at: 0 113 1730 1854 2292
Size: 113 1617 124 438
Fragments arranged by size:
1617 438 124 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 489 1908 2292
Size: 489 1419 384
Fragments arranged by size:
1419 489 384
BtsI GCAGTG_nn'
Cuts at: 0 409 535 667 2292
Size: 409 126 132 1625
Fragments arranged by size:
1625 409 132 126
DraIII CAC_nnn'GTG
Cuts at: 0 989 2292
Size: 989 1303
EaeI y'GGCC_r
Cuts at: 0 582 644 899 1469 2292
Size: 582 62 255 570 823
Fragments arranged by size:
823 582 570 255 62
EagI C'GGCC_G
Cuts at: 0 582 2292
Size: 582 1710
EarI CTCTTCn'nnn_
Cuts at: 0 241 511 1423 1804 2292
Size: 241 270 912 381 488
Fragments arranged by size:
912 488 381 270 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 2292
Size: 88 2204
EcoICRI GAG'CTC
Cuts at: 0 1797 2292
Size: 1797 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 706 913 1084 1264 1989 2026 2094
Size: 5 701 207 171 180 725 37 68
Cuts at: 2094 2266 2292
Size: 172 26
Fragments arranged by size:
725 701 207 180 172 171 68 37
26 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 1426 1669 2292
Size: 361 1065 243 623
Fragments arranged by size:
1065 623 361 243
EcoO109I rG'GnC_Cy
Cuts at: 0 830 1431 1599 1702 1760 2169 2292
Size: 830 601 168 103 58 409 123
Fragments arranged by size:
830 601 409 168 123 103 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1191 2292
Size: 1191 1101
HaeII r_GCGC'y
Cuts at: 0 497 1371 1686 2103 2292
Size: 497 874 315 417 189
Fragments arranged by size:
874 497 417 315 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 2158 2190 2233 2265 2292
Size: 415 32 1711 32 43 32 27
Fragments arranged by size:
1711 415 43 32 32 32 27
HincII GTy'rAC
Cuts at: 0 168 1947 2292
Size: 168 1779 345
Fragments arranged by size:
1779 345 168
KasI G'GCGC_C
Cuts at: 0 1367 1682 2292
Size: 1367 315 610
Fragments arranged by size:
1367 610 315
KpnI G_GTAC'C
Cuts at: 0 1485 2292
Size: 1485 807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 1260 2292
Size: 14 1246 1032
Fragments arranged by size:
1246 1032 14
MscI TGG'CCA
Cuts at: 0 1471 2292
Size: 1471 821
MslI CAynn'nnrTG
Cuts at: 0 1444 1816 2292
Size: 1444 372 476
Fragments arranged by size:
1444 476 372
MspA1I CmG'CkG
Cuts at: 0 778 945 1043 1219 1350 1845 1856 1967
Size: 778 167 98 176 131 495 11 111
Cuts at: 1967 2046 2292
Size: 79 246
Fragments arranged by size:
778 495 246 176 167 131 111 98
79 11
NarI GG'CG_CC
Cuts at: 0 1368 1683 2292
Size: 1368 315 609
Fragments arranged by size:
1368 609 315
NcoI C'CATG_G
Cuts at: 0 113 1730 2292
Size: 113 1617 562
Fragments arranged by size:
1617 562 113
NotI GC'GGCC_GC
Cuts at: 0 582 2292
Size: 582 1710
NspI r_CATG'y
Cuts at: 0 571 751 807 1206 1920 2292
Size: 571 180 56 399 714 372
Fragments arranged by size:
714 571 399 372 180 56
PasI CC'CwG_GG
Cuts at: 0 729 2292
Size: 729 1563
PciI A'CATG_T
Cuts at: 0 1202 2292
Size: 1202 1090
PflMI CCAn_nnn'nTGG
Cuts at: 0 662 1478 2292
Size: 662 816 814
Fragments arranged by size:
816 814 662
PfoI T'CCnGG_A
Cuts at: 0 683 1778 1835 2003 2292
Size: 683 1095 57 168 289
Fragments arranged by size:
1095 683 289 168 57
PmlI CAC'GTG
Cuts at: 0 1374 1543 2292
Size: 1374 169 749
Fragments arranged by size:
1374 749 169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 2292
Size: 109 32 2151
Fragments arranged by size:
2151 109 32
PpuMI rG'GwC_Cy
Cuts at: 0 1599 2169 2292
Size: 1599 570 123
Fragments arranged by size:
1599 570 123
PspOMI G'GGCC_C
Cuts at: 0 830 1760 2292
Size: 830 930 532
Fragments arranged by size:
930 830 532
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 2292
Size: 1516 32 744
Fragments arranged by size:
1516 744 32
PstI C_TGCA'G
Cuts at: 0 419 530 716 878 944 1142 1988 2292
Size: 419 111 186 162 66 198 846 304
Fragments arranged by size:
846 419 304 198 186 162 111 66
PvuII CAG'CTG
Cuts at: 0 778 1043 1350 1845 1967 2292
Size: 778 265 307 495 122 325
Fragments arranged by size:
778 495 325 307 265 122
SacI G_AGCT'C
Cuts at: 0 1799 2292
Size: 1799 493
SacII CC_GC'GG
Cuts at: 0 1857 2292
Size: 1857 435
SapI GCTCTTCn'nnn_
Cuts at: 0 241 1804 2292
Size: 241 1563 488
Fragments arranged by size:
1563 488 241
SbfI CC_TGCA'GG
Cuts at: 0 716 878 2292
Size: 716 162 1414
Fragments arranged by size:
1414 716 162
SfcI C'TryA_G
Cuts at: 0 415 526 712 874 940 1138 1566 1649
Size: 415 111 186 162 66 198 428 83
Cuts at: 1649 1697 1984 2292
Size: 48 287 308
Fragments arranged by size:
428 415 308 287 198 186 162 111
83 66 48
SfoI GGC'GCC
Cuts at: 0 1369 1684 2292
Size: 1369 315 608
Fragments arranged by size:
1369 608 315
SmaI CCC'GGG
Cuts at: 0 253 574 2292
Size: 253 321 1718
Fragments arranged by size:
1718 321 253
SmlI C'TyrA_G
Cuts at: 0 379 856 2200 2292
Size: 379 477 1344 92
Fragments arranged by size:
1344 477 379 92
SphI G_CATG'C
Cuts at: 0 571 751 2292
Size: 571 180 1541
Fragments arranged by size:
1541 571 180
SrfI GCCC'GGGC
Cuts at: 0 574 2292
Size: 574 1718
StyI C'CwwG_G
Cuts at: 0 113 603 905 1730 1790 2228 2292
Size: 113 490 302 825 60 438 64
Fragments arranged by size:
825 490 438 302 113 64 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 2292
Size: 239 2053
TatI w'GTAC_w
Cuts at: 0 1766 2292
Size: 1766 526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1532 1564 2292
Size: 1532 32 728
Fragments arranged by size:
1532 728 32
Tth111I GACn'n_nGTC
Cuts at: 0 388 2292
Size: 388 1904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2001 2292
Size: 2001 291
XhoI C'TCGA_G
Cuts at: 0 2200 2292
Size: 2200 92
XmaI C'CCGG_G
Cuts at: 0 251 572 2292
Size: 251 321 1720
Fragments arranged by size:
1720 321 251
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NotI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SapI SbfI SfcI SfoI SmaI SmlI SphI
SrfI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI AclI AgeI AhdI AscI AseI AsiSI
AvrII BcgI BclI BfrBI BglI BglII BlpI BmtI
BsaBI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NruI NsiI PacI PmeI PshAI
PsiI PspXI PvuI RsrII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SspI StuI SwaI
XbaI XmnI ZraI