Restriction map for
pFN21AE4576
(Linear) (Six-base) MAPSORT of: KIEE4576.seq Check: 9890 from: 1 to: 2289
KIEE4576.orf
With 182 enzymes: SgfI *
March 22, 2012 18:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 289 2097 2289
Size: 289 1808 192
Fragments arranged by size:
1808 289 192
Acc65I G'GTAC_C
Cuts at: 0 508 2289
Size: 508 1781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 536 1260 1553 1688 1996 2015 2289
Size: 30 506 724 293 135 308 19 274
Fragments arranged by size:
724 506 308 293 274 135 30 19
AflII C'TTAA_G
Cuts at: 0 1360 2289
Size: 1360 929
AflIII A'CryG_T
Cuts at: 0 314 1791 2289
Size: 314 1477 498
Fragments arranged by size:
1477 498 314
AleI CACnn'nnGTG
Cuts at: 0 1759 2289
Size: 1759 530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2076 2289
Size: 2076 213
AlwNI CAG_nnn'CTG
Cuts at: 0 304 1041 1524 2289
Size: 304 737 483 765
Fragments arranged by size:
765 737 483 304
ApoI r'AATT_y
Cuts at: 0 976 1057 1944 2289
Size: 976 81 887 345
Fragments arranged by size:
976 887 345 81
AvaI C'yCGr_G
Cuts at: 0 62 2289
Size: 62 2227
AvrII C'CTAG_G
Cuts at: 0 1881 2289
Size: 1881 408
BamHI G'GATC_C
Cuts at: 0 50 427 2289
Size: 50 377 1862
Fragments arranged by size:
1862 377 50
BanI G'GyrC_C
Cuts at: 0 508 1747 2173 2289
Size: 508 1239 426 116
Fragments arranged by size:
1239 508 426 116
BanII G_rGCy'C
Cuts at: 0 1068 2289
Size: 1068 1221
BbeI G_GCGC'C
Cuts at: 0 2177 2289
Size: 2177 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 2018 2289
Size: 86 1932 271
Fragments arranged by size:
1932 271 86
BbvCI CC'TCA_GC
Cuts at: 0 28 2289
Size: 28 2261
BclI T'GATC_A
Cuts at: 0 246 2289
Size: 246 2043
BglI GCCn_nnn'nGGC
Cuts at: 0 13 2289
Size: 13 2276
BglII A'GATC_T
Cuts at: 0 764 1803 2289
Size: 764 1039 486
Fragments arranged by size:
1039 764 486
BmrI ACTGGGnnnn_n'
Cuts at: 0 1298 2289
Size: 1298 991
BmtI G_CTAG'C
Cuts at: 0 2251 2289
Size: 2251 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1546 1669 2289
Size: 677 869 123 620
Fragments arranged by size:
869 677 620 123
Bpu10I CC'TnA_GC
Cuts at: 0 28 2289
Size: 28 2261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 1573 2182 2289
Size: 469 1104 609 107
Fragments arranged by size:
1104 609 469 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1591 2289
Size: 1591 698
BsaBI GATnn'nnATC
Cuts at: 0 22 2289
Size: 22 2267
BsaHI Gr'CG_yC
Cuts at: 0 2174 2289
Size: 2174 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 974 1004 2289
Size: 974 30 1285
Fragments arranged by size:
1285 974 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 455 2289
Size: 455 1834
BseYI C'CCAG_C
Cuts at: 0 525 2289
Size: 525 1764
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 881 1124 2289
Size: 644 237 243 1165
Fragments arranged by size:
1165 644 243 237
BsiEI CG_ry'CG
Cuts at: 0 1541 2289
Size: 1541 748
BsiHKAI G_wGCw'C
Cuts at: 0 1068 2289
Size: 1068 1221
BsmI GAATG_Cn'
Cuts at: 0 617 2289
Size: 617 1672
Bsp1286I G_dGCh'C
Cuts at: 0 1068 2289
Size: 1068 1221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 2097 2289
Size: 289 1808 192
Fragments arranged by size:
1808 289 192
BsrBI CCG'CTC
Cuts at: 0 59 1827 2289
Size: 59 1768 462
Fragments arranged by size:
1768 462 59
BsrFI r'CCGG_y
Cuts at: 0 952 2192 2289
Size: 952 1240 97
Fragments arranged by size:
1240 952 97
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1371 2289
Size: 1371 918
BstBI TT'CG_AA
Cuts at: 0 156 2289
Size: 156 2133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1761 2289
Size: 573 1188 528
Fragments arranged by size:
1188 573 528
BstYI r'GATC_y
Cuts at: 0 50 427 764 1803 2075 2163 2289
Size: 50 377 337 1039 272 88 126
Fragments arranged by size:
1039 377 337 272 126 88 50
BtgI C'CryG_G
Cuts at: 0 1029 1139 1505 2289
Size: 1029 110 366 784
Fragments arranged by size:
1029 784 366 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1110 2289
Size: 1110 1179
BtsI GCAGTG_nn'
Cuts at: 0 1181 2289
Size: 1181 1108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1713 1748 2289
Size: 1713 35 541
Fragments arranged by size:
1713 541 35
DraIII CAC_nnn'GTG
Cuts at: 0 1371 2289
Size: 1371 918
EaeI y'GGCC_r
Cuts at: 0 2081 2289
Size: 2081 208
EarI CTCTTCn'nnn_
Cuts at: 0 1924 2289
Size: 1924 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1813 2014 2289
Size: 1813 201 275
Fragments arranged by size:
1813 275 201
EcoICRI GAG'CTC
Cuts at: 0 1066 2289
Size: 1066 1223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 536 677 1260 1546 1553 1669 1688
Size: 30 506 141 583 286 7 116 19
Cuts at: 1688 1996 2015 2289
Size: 308 19 274
Fragments arranged by size:
583 506 308 286 274 141 116 30
19 19 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2068 2289
Size: 2068 221
EcoO109I rG'GnC_Cy
Cuts at: 0 530 1038 1350 2146 2289
Size: 530 508 312 796 143
Fragments arranged by size:
796 530 508 312 143
EcoRI G'AATT_C
Cuts at: 0 1944 2289
Size: 1944 345
EcoRV GAT'ATC
Cuts at: 0 309 2289
Size: 309 1980
HaeII r_GCGC'y
Cuts at: 0 2177 2289
Size: 2177 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1109 1141 1386 1418 2289
Size: 1109 32 245 32 871
Fragments arranged by size:
1109 871 245 32 32
HincII GTy'rAC
Cuts at: 0 654 1698 2289
Size: 654 1044 591
Fragments arranged by size:
1044 654 591
HindIII A'AGCT_T
Cuts at: 0 1728 2289
Size: 1728 561
KasI G'GCGC_C
Cuts at: 0 2173 2289
Size: 2173 116
KpnI G_GTAC'C
Cuts at: 0 512 2289
Size: 512 1777
MscI TGG'CCA
Cuts at: 0 2083 2289
Size: 2083 206
MslI CAynn'nnrTG
Cuts at: 0 1609 1759 2289
Size: 1609 150 530
Fragments arranged by size:
1609 530 150
MspA1I CmG'CkG
Cuts at: 0 1521 1573 2244 2289
Size: 1521 52 671 45
Fragments arranged by size:
1521 671 52 45
NarI GG'CG_CC
Cuts at: 0 2174 2289
Size: 2174 115
NcoI C'CATG_G
Cuts at: 0 1029 2289
Size: 1029 1260
NheI G'CTAG_C
Cuts at: 0 2247 2289
Size: 2247 42
NspI r_CATG'y
Cuts at: 0 318 1491 1795 2289
Size: 318 1173 304 494
Fragments arranged by size:
1173 494 318 304
PasI CC'CwG_GG
Cuts at: 0 672 2289
Size: 672 1617
PciI A'CATG_T
Cuts at: 0 314 1791 2289
Size: 314 1477 498
Fragments arranged by size:
1477 498 314
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 2289
Size: 663 1626
PfoI T'CCnGG_A
Cuts at: 0 1685 2289
Size: 1685 604
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1350 2289
Size: 1038 312 939
Fragments arranged by size:
1038 939 312
PshAI GACnn'nnGTC
Cuts at: 0 1355 2289
Size: 1355 934
PstI C_TGCA'G
Cuts at: 0 2094 2289
Size: 2094 195
PvuII CAG'CTG
Cuts at: 0 1521 1573 2244 2289
Size: 1521 52 671 45
Fragments arranged by size:
1521 671 52 45
SacI G_AGCT'C
Cuts at: 0 1068 2289
Size: 1068 1221
SexAI A'CCwGG_T
Cuts at: 0 1642 2289
Size: 1642 647
SfcI C'TryA_G
Cuts at: 0 1574 2090 2289
Size: 1574 516 199
Fragments arranged by size:
1574 516 199
SfoI GGC'GCC
Cuts at: 0 2175 2289
Size: 2175 114
SmlI C'TyrA_G
Cuts at: 0 448 1360 1588 2197 2289
Size: 448 912 228 609 92
Fragments arranged by size:
912 609 448 228 92
SphI G_CATG'C
Cuts at: 0 1491 2289
Size: 1491 798
StuI AGG'CCT
Cuts at: 0 887 1012 2289
Size: 887 125 1277
Fragments arranged by size:
1277 887 125
StyI C'CwwG_G
Cuts at: 0 1029 1305 1871 1881 2289
Size: 1029 276 566 10 408
Fragments arranged by size:
1029 566 408 276 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 565 1208 1555 1735 2289
Size: 565 643 347 180 554
Fragments arranged by size:
643 565 554 347 180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 124 2289
Size: 124 2165
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BclI BglI BglII BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII HindIII KasI KpnI MscI
MslI MspA1I NarI NcoI NheI NspI PasI PciI
PflMI PfoI PpuMI PshAI PstI PvuII SacI SexAI
SfcI SfoI SmlI SphI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI BaeI BcgI BciVI BfrBI
BlpI Bme1580I BmgBI BplI BsaAI BsaWI BsiWI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I
Bsu36I ClaI DraI DrdI EagI FalI FseI FspI
FspAI HpaI MfeI MluI MmeI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI