Restriction map for
pFN21AE4573
(Linear) (Six-base) MAPSORT of: KIEE4573.seq Check: 7802 from: 1 to: 2226
KIEE4573.orf
With 182 enzymes: SgfI *
March 22, 2012 18:25 ..
AclI AA'CG_TT
Cuts at: 0 1085 2226
Size: 1085 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 776 1143 2104 2226
Size: 669 107 367 961 122
Fragments arranged by size:
961 669 367 122 107
AflIII A'CryG_T
Cuts at: 0 834 2226
Size: 834 1392
AhdI GACnn_n'nnGTC
Cuts at: 0 765 2226
Size: 765 1461
AleI CACnn'nnGTG
Cuts at: 0 1183 2226
Size: 1183 1043
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2051 2083 2226
Size: 2051 32 143
Fragments arranged by size:
2051 143 32
AlwNI CAG_nnn'CTG
Cuts at: 0 229 949 2226
Size: 229 720 1277
Fragments arranged by size:
1277 720 229
ApaI G_GGCC'C
Cuts at: 0 1176 1377 1455 1656 1797 2139 2226
Size: 1176 201 78 201 141 342 87
Fragments arranged by size:
1176 342 201 201 141 87 78
ApoI r'AATT_y
Cuts at: 0 286 453 1130 2226
Size: 286 167 677 1096
Fragments arranged by size:
1096 677 286 167
AvaI C'yCGr_G
Cuts at: 0 251 1447 2226
Size: 251 1196 779
Fragments arranged by size:
1196 779 251
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1056 1089 2226
Size: 1056 33 1137
Fragments arranged by size:
1137 1056 33
BamHI G'GATC_C
Cuts at: 0 256 2226
Size: 256 1970
BanII G_rGCy'C
Cuts at: 0 1167 1176 1377 1446 1455 1656 1797 1904
Size: 1167 9 201 69 9 201 141 107
Cuts at: 1904 2139 2226
Size: 235 87
Fragments arranged by size:
1167 235 201 201 141 107 87 69
9 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 2095 2226
Size: 764 1331 131
Fragments arranged by size:
1331 764 131
BbvCI CC'TCA_GC
Cuts at: 0 1102 2226
Size: 1102 1124
BfrBI ATG'CAT
Cuts at: 0 2043 2226
Size: 2043 183
BglII A'GATC_T
Cuts at: 0 823 1991 2226
Size: 823 1168 235
Fragments arranged by size:
1168 823 235
BlpI GC'TnA_GC
Cuts at: 0 1281 1744 2226
Size: 1281 463 482
Fragments arranged by size:
1281 482 463
Bme1580I G_kGCm'C
Cuts at: 0 1176 1377 1455 1656 1797 2139 2226
Size: 1176 201 78 201 141 342 87
Fragments arranged by size:
1176 342 201 201 141 87 78
BmtI G_CTAG'C
Cuts at: 0 2123 2226
Size: 2123 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1244 1414 1884 2226
Size: 1244 170 470 342
Fragments arranged by size:
1244 470 342 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 718 735 1219 1313 1498 1592 1957
Size: 447 271 17 484 94 185 94 365
Cuts at: 1957 1985 2016 2226
Size: 28 31 210
Fragments arranged by size:
484 447 365 271 210 185 94 94
31 28 17
Bpu10I CC'TnA_GC
Cuts at: 0 1102 2226
Size: 1102 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1880 2226
Size: 1880 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 2226
Size: 628 1598
BsaAI yAC'GTr
Cuts at: 0 835 1109 2226
Size: 835 274 1117
Fragments arranged by size:
1117 835 274
BsaWI w'CCGG_w
Cuts at: 0 883 1134 1413 2226
Size: 883 251 279 813
Fragments arranged by size:
883 813 279 251
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 101 131 329 359 1930 1960 2226
Size: 101 30 198 30 1571 30 266
Fragments arranged by size:
1571 266 198 101 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1310 1313 1589 2226
Size: 67 1243 3 276 637
Fragments arranged by size:
1243 637 276 67 3
BseYI C'CCAG_C
Cuts at: 0 638 1004 1376 1655 2226
Size: 638 366 372 279 571
Fragments arranged by size:
638 571 372 366 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 2226
Size: 1094 1132
BsiHKAI G_wGCw'C
Cuts at: 0 1745 2226
Size: 1745 481
Bsp1286I G_dGCh'C
Cuts at: 0 1167 1176 1377 1446 1455 1656 1745 1797
Size: 1167 9 201 69 9 201 89 52
Cuts at: 1797 1904 2139 2226
Size: 107 235 87
Fragments arranged by size:
1167 235 201 201 107 89 87 69
52 9 9
BspHI T'CATG_A
Cuts at: 0 1254 2226
Size: 1254 972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 710 1202 1481 2226
Size: 710 492 279 745
Fragments arranged by size:
745 710 492 279
BsrDI GCAATG_nn'
Cuts at: 0 1959 2226
Size: 1959 267
BsrGI T'GTAC_A
Cuts at: 0 837 1946 2226
Size: 837 1109 280
Fragments arranged by size:
1109 837 280
BssSI C'ACGA_G
Cuts at: 0 1534 2226
Size: 1534 692
BstYI r'GATC_y
Cuts at: 0 212 256 737 823 872 1991 2226
Size: 212 44 481 86 49 1119 235
Fragments arranged by size:
1119 481 235 212 86 49 44
Bsu36I CC'TnA_GG
Cuts at: 0 1438 2226
Size: 1438 788
BtgI C'CryG_G
Cuts at: 0 88 2226
Size: 88 2138
DraIII CAC_nnn'GTG
Cuts at: 0 1182 2226
Size: 1182 1044
EarI CTCTTCn'nnn_
Cuts at: 0 33 597 688 972 2226
Size: 33 564 91 284 1254
Fragments arranged by size:
1254 564 284 91 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 669 718 735 776 1143 1219 1313
Size: 447 222 49 17 41 367 76 94
Cuts at: 1313 1498 1592 1957 1985 2016 2104 2226
Size: 185 94 365 28 31 88 122
Fragments arranged by size:
447 367 365 222 185 122 94 94
88 76 49 41 31 28 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 1872 2226
Size: 154 1718 354
Fragments arranged by size:
1718 354 154
EcoO109I rG'GnC_Cy
Cuts at: 0 722 1172 1191 1373 1451 1470 1652 1793
Size: 722 450 19 182 78 19 182 141
Cuts at: 1793 2135 2226
Size: 342 91
Fragments arranged by size:
722 450 342 182 182 141 91 78
19 19
EcoRI G'AATT_C
Cuts at: 0 453 1130 2226
Size: 453 677 1096
Fragments arranged by size:
1096 677 453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 2226
Size: 88 2138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 248 280 329 361 722 751
Size: 15 32 201 32 49 32 361 29
Cuts at: 751 754 780 783 812 1878 1910 2226
Size: 3 26 3 29 1066 32 316
Fragments arranged by size:
1066 361 316 201 49 32 32 32
32 29 29 26 15 3 3
MslI CAynn'nnrTG
Cuts at: 0 1183 1259 1385 1538 2226
Size: 1183 76 126 153 688
Fragments arranged by size:
1183 688 153 126 76
MspA1I CmG'CkG
Cuts at: 0 669 894 1078 1720 2226
Size: 669 225 184 642 506
Fragments arranged by size:
669 642 506 225 184
NcoI C'CATG_G
Cuts at: 0 88 2226
Size: 88 2138
NdeI CA'TA_TG
Cuts at: 0 127 2226
Size: 127 2099
NheI G'CTAG_C
Cuts at: 0 2119 2226
Size: 2119 107
NsiI A_TGCA'T
Cuts at: 0 2045 2226
Size: 2045 181
NspI r_CATG'y
Cuts at: 0 283 1033 2226
Size: 283 750 1193
Fragments arranged by size:
1193 750 283
PasI CC'CwG_GG
Cuts at: 0 156 936 1169 1269 2226
Size: 156 780 233 100 957
Fragments arranged by size:
957 780 233 156 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 1182 1461 2226
Size: 1182 279 765
Fragments arranged by size:
1182 765 279
PfoI T'CCnGG_A
Cuts at: 0 33 1696 1882 1962 2226
Size: 33 1663 186 80 264
Fragments arranged by size:
1663 264 186 80 33
PmlI CAC'GTG
Cuts at: 0 1109 2226
Size: 1109 1117
PpuMI rG'GwC_Cy
Cuts at: 0 722 1191 1470 2226
Size: 722 469 279 756
Fragments arranged by size:
756 722 469 279
PsiI TTA'TAA
Cuts at: 0 431 2226
Size: 431 1795
PspOMI G'GGCC_C
Cuts at: 0 1172 1373 1451 1652 1793 2135 2226
Size: 1172 201 78 201 141 342 91
Fragments arranged by size:
1172 342 201 201 141 91 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1698 1730 2226
Size: 1698 32 496
Fragments arranged by size:
1698 496 32
PstI C_TGCA'G
Cuts at: 0 314 1329 2112 2226
Size: 314 1015 783 114
Fragments arranged by size:
1015 783 314 114
PvuII CAG'CTG
Cuts at: 0 669 894 1078 2226
Size: 669 225 184 1148
Fragments arranged by size:
1148 669 225 184
SapI GCTCTTCn'nnn_
Cuts at: 0 597 2226
Size: 597 1629
SbfI CC_TGCA'GG
Cuts at: 0 314 2226
Size: 314 1912
SfcI C'TryA_G
Cuts at: 0 310 1325 1604 2108 2226
Size: 310 1015 279 504 118
Fragments arranged by size:
1015 504 310 279 118
SmaI CCC'GGG
Cuts at: 0 1449 2226
Size: 1449 777
SmlI C'TyrA_G
Cuts at: 0 1895 2226
Size: 1895 331
SspI AAT'ATT
Cuts at: 0 2172 2226
Size: 2172 54
StuI AGG'CCT
Cuts at: 0 1712 1878 2226
Size: 1712 166 348
Fragments arranged by size:
1712 348 166
StyI C'CwwG_G
Cuts at: 0 88 1547 2226
Size: 88 1459 679
Fragments arranged by size:
1459 679 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2111 2226
Size: 2111 115
TatI w'GTAC_w
Cuts at: 0 69 487 837 1520 1946 2226
Size: 69 418 350 683 426 280
Fragments arranged by size:
683 426 418 350 280 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 101 133 1198 1230 1477 1509 2226
Size: 101 32 1065 32 247 32 717
Fragments arranged by size:
1065 717 247 101 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 343 2226
Size: 343 1883
XbaI T'CTAG_A
Cuts at: 0 867 2226
Size: 867 1359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1384 2226
Size: 1384 842
XmaI C'CCGG_G
Cuts at: 0 1447 2226
Size: 1447 779
Enzymes that do cut:
AclI AcuI AflIII AhdI AleI AloI AlwNI ApaI
ApoI AvaI BaeI BamHI BanII BbsI BbvCI BfrBI
BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstYI Bsu36I
BtgI DraIII EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I MslI MspA1I NcoI NdeI NheI NsiI NspI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI
PstI PvuII SapI SbfI SfcI SmaI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AlfI
ApaLI AscI AseI AsiSI AvrII BanI BbeI BcgI
BciVI BclI BglI BmgBI BmrI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NgoMIV NotI
NruI PacI PciI PmeI PpiI PshAI PspXI PvuI
RsrII SacI SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
XhoI XmnI ZraI