Restriction map for
pFN21AE4569
(Linear) (Six-base) MAPSORT of: KIEE4569.seq Check: 1198 from: 1 to: 2172
KIEE4569.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AatII G_ACGT'C
Cuts at: 0 284 2172
Size: 284 1888
AccI GT'mk_AC
Cuts at: 0 1442 2172
Size: 1442 730
Acc65I G'GTAC_C
Cuts at: 0 1068 2172
Size: 1068 1104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1476 1493 2172
Size: 1476 17 679
Fragments arranged by size:
1476 679 17
AfeI AGC'GCT
Cuts at: 0 580 2172
Size: 580 1592
AflIII A'CryG_T
Cuts at: 0 110 1406 1454 2172
Size: 110 1296 48 718
Fragments arranged by size:
1296 718 110 48
AhdI GACnn_n'nnGTC
Cuts at: 0 552 1881 1938 2172
Size: 552 1329 57 234
Fragments arranged by size:
1329 552 234 57
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 2172
Size: 64 32 2076
Fragments arranged by size:
2076 64 32
ApaI G_GGCC'C
Cuts at: 0 2008 2172
Size: 2008 164
AvaI C'yCGr_G
Cuts at: 0 314 339 500 1232 1837 2172
Size: 314 25 161 732 605 335
Fragments arranged by size:
732 605 335 314 161 25
AvrII C'CTAG_G
Cuts at: 0 1063 2172
Size: 1063 1109
BamHI G'GATC_C
Cuts at: 0 689 1037 2172
Size: 689 348 1135
Fragments arranged by size:
1135 689 348
BanI G'GyrC_C
Cuts at: 0 1068 1251 1359 2172
Size: 1068 183 108 813
Fragments arranged by size:
1068 813 183 108
BanII G_rGCy'C
Cuts at: 0 508 2008 2172
Size: 508 1500 164
Fragments arranged by size:
1500 508 164
BbsI GAAGACnn'nnnn_
Cuts at: 0 1817 2064 2172
Size: 1817 247 108
Fragments arranged by size:
1817 247 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 826 860 1408 1442 2172
Size: 826 34 548 34 730
Fragments arranged by size:
826 730 548 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 248 533 593 2172
Size: 248 285 60 1579
Fragments arranged by size:
1579 285 248 60
Bme1580I G_kGCm'C
Cuts at: 0 788 1266 1654 2008 2172
Size: 788 478 388 354 164
Fragments arranged by size:
788 478 388 354 164
BmgBI CAC'GTC
Cuts at: 0 42 1407 1455 2172
Size: 42 1365 48 717
Fragments arranged by size:
1365 717 48 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1560 2172
Size: 1560 612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1704 2172
Size: 1704 468
Bpu10I CC'TnA_GC
Cuts at: 0 2009 2172
Size: 2009 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 2172
Size: 1795 377
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 2143 2172
Size: 448 1695 29
Fragments arranged by size:
1695 448 29
BsaAI yAC'GTr
Cuts at: 0 1731 2172
Size: 1731 441
BsaHI Gr'CG_yC
Cuts at: 0 281 553 830 2172
Size: 281 272 277 1342
Fragments arranged by size:
1342 281 277 272
BsaWI w'CCGG_w
Cuts at: 0 840 1034 1461 2172
Size: 840 194 427 711
Fragments arranged by size:
840 711 427 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 742 772 1744 1774 2172
Size: 64 30 648 30 972 30 398
Fragments arranged by size:
972 648 398 64 30 30 30
BseYI C'CCAG_C
Cuts at: 0 148 601 674 2172
Size: 148 453 73 1498
Fragments arranged by size:
1498 453 148 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1603 2172
Size: 258 1345 569
Fragments arranged by size:
1345 569 258
BsiEI CG_ry'CG
Cuts at: 0 655 999 1035 2172
Size: 655 344 36 1137
Fragments arranged by size:
1137 655 344 36
BsiHKAI G_wGCw'C
Cuts at: 0 1562 2172
Size: 1562 610
BsmBI CGTCTCn'nnnn_
Cuts at: 0 821 1208 2172
Size: 821 387 964
Fragments arranged by size:
964 821 387
Bsp1286I G_dGCh'C
Cuts at: 0 508 788 1266 1562 1654 2008 2172
Size: 508 280 478 296 92 354 164
Fragments arranged by size:
508 478 354 296 280 164 92
BspEI T'CCGG_A
Cuts at: 0 840 1461 2172
Size: 840 621 711
Fragments arranged by size:
840 711 621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 417 2117 2172
Size: 54 363 1700 55
Fragments arranged by size:
1700 363 55 54
BsrBI CCG'CTC
Cuts at: 0 221 2172
Size: 221 1951
BsrDI GCAATG_nn'
Cuts at: 0 2130 2172
Size: 2130 42
BsrFI r'CCGG_y
Cuts at: 0 139 679 953 1964 2172
Size: 139 540 274 1011 208
Fragments arranged by size:
1011 540 274 208 139
BssHII G'CGCG_C
Cuts at: 0 173 224 226 570 2172
Size: 173 51 2 344 1602
Fragments arranged by size:
1602 344 173 51 2
BssSI C'ACGA_G
Cuts at: 0 613 2172
Size: 613 1559
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 2172
Size: 42 2130
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1554 2172
Size: 1554 618
BstYI r'GATC_y
Cuts at: 0 689 1037 2172
Size: 689 348 1135
Fragments arranged by size:
1135 689 348
BtgI C'CryG_G
Cuts at: 0 245 1247 2172
Size: 245 1002 925
Fragments arranged by size:
1002 925 245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 656 830 950 1019 1115 2172
Size: 656 174 120 69 96 1057
Fragments arranged by size:
1057 656 174 120 96 69
BtsI GCAGTG_nn'
Cuts at: 0 168 270 1622 2172
Size: 168 102 1352 550
Fragments arranged by size:
1352 550 168 102
EaeI y'GGCC_r
Cuts at: 0 996 1287 1371 1790 2018 2058 2172
Size: 996 291 84 419 228 40 114
Fragments arranged by size:
996 419 291 228 114 84 40
EagI C'GGCC_G
Cuts at: 0 996 2172
Size: 996 1176
EarI CTCTTCn'nnn_
Cuts at: 0 1132 2172
Size: 1132 1040
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1171 2172
Size: 1171 1001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1476 1493 1704 2172
Size: 1476 17 211 468
Fragments arranged by size:
1476 468 211 17
EcoO109I rG'GnC_Cy
Cuts at: 0 861 1496 1834 1908 2004 2005 2172
Size: 861 635 338 74 96 1 167
Fragments arranged by size:
861 635 338 167 96 74 1
HaeII r_GCGC'y
Cuts at: 0 34 582 756 2172
Size: 34 548 174 1416
Fragments arranged by size:
1416 548 174 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1867 1899 1924 1956 2172
Size: 538 32 1297 32 25 32 216
Fragments arranged by size:
1297 538 216 32 32 32 25
HincII GTy'rAC
Cuts at: 0 285 2172
Size: 285 1887
KpnI G_GTAC'C
Cuts at: 0 1072 2172
Size: 1072 1100
MluI A'CGCG_T
Cuts at: 0 110 2172
Size: 110 2062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1830 1906 2172
Size: 1830 76 266
Fragments arranged by size:
1830 266 76
MscI TGG'CCA
Cuts at: 0 1289 2172
Size: 1289 883
MslI CAynn'nnrTG
Cuts at: 0 97 617 1987 2047 2172
Size: 97 520 1370 60 125
Fragments arranged by size:
1370 520 125 97 60
MspA1I CmG'CkG
Cuts at: 0 247 323 350 377 567 888 1848 2120
Size: 247 76 27 27 190 321 960 272
Cuts at: 2120 2160 2172
Size: 40 12
Fragments arranged by size:
960 321 272 247 190 76 40 27
27 12
NaeI GCC'GGC
Cuts at: 0 681 2172
Size: 681 1491
NgoMIV G'CCGG_C
Cuts at: 0 679 2172
Size: 679 1493
NruI TCG'CGA
Cuts at: 0 704 2172
Size: 704 1468
NspI r_CATG'y
Cuts at: 0 622 2172
Size: 622 1550
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 2172
Size: 396 1776
PfoI T'CCnGG_A
Cuts at: 0 725 2172
Size: 725 1447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1639 1671 2172
Size: 64 32 1543 32 501
Fragments arranged by size:
1543 501 64 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 861 1908 2172
Size: 861 1047 264
Fragments arranged by size:
1047 861 264
PshAI GACnn'nnGTC
Cuts at: 0 1127 1241 1457 2172
Size: 1127 114 216 715
Fragments arranged by size:
1127 715 216 114
PspOMI G'GGCC_C
Cuts at: 0 2004 2172
Size: 2004 168
PstI C_TGCA'G
Cuts at: 0 17 38 1961 2172
Size: 17 21 1923 211
Fragments arranged by size:
1923 211 21 17
PvuII CAG'CTG
Cuts at: 0 1848 2160 2172
Size: 1848 312 12
Fragments arranged by size:
1848 312 12
SacII CC_GC'GG
Cuts at: 0 248 2172
Size: 248 1924
SanDI GG'GwC_CC
Cuts at: 0 861 2172
Size: 861 1311
SapI GCTCTTCn'nnn_
Cuts at: 0 1132 2172
Size: 1132 1040
SfcI C'TryA_G
Cuts at: 0 13 34 1957 2172
Size: 13 21 1923 215
Fragments arranged by size:
1923 215 21 13
SmaI CCC'GGG
Cuts at: 0 341 502 1839 2172
Size: 341 161 1337 333
Fragments arranged by size:
1337 341 333 161
SmlI C'TyrA_G
Cuts at: 0 1810 2172
Size: 1810 362
SphI G_CATG'C
Cuts at: 0 622 2172
Size: 622 1550
StuI AGG'CCT
Cuts at: 0 1607 2172
Size: 1607 565
StyI C'CwwG_G
Cuts at: 0 413 1063 2172
Size: 413 650 1109
Fragments arranged by size:
1109 650 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 400 1542 2172
Size: 400 1142 630
Fragments arranged by size:
1142 630 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 742 774 2172
Size: 681 32 29 32 1398
Fragments arranged by size:
1398 681 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 628 859 1327 2172
Size: 628 231 468 845
Fragments arranged by size:
845 628 468 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1728 2172
Size: 1728 444
XmaI C'CCGG_G
Cuts at: 0 339 500 1837 2172
Size: 339 161 1337 335
Fragments arranged by size:
1337 339 335 161
XmnI GAAnn'nnTTC
Cuts at: 0 1777 2172
Size: 1777 395
ZraI GAC'GTC
Cuts at: 0 282 2172
Size: 282 1890
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AhdI AloI
ApaI AvaI AvrII BamHI BanI BanII BbsI BcgI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII
KpnI MluI MmeI MscI MslI MspA1I NaeI NgoMIV
NruI NspI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI PvuII SacII SanDI SapI SfcI SmaI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AleI AlfI AlwNI ApaLI
ApoI AscI AseI AsiSI BaeI BbeI BbvCI BciVI
BclI BfrBI BglII BlpI BmtI BplI BsaBI BseRI
BsiWI BsmI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI NarI NcoI NdeI NheI NotI NsiI PacI
PasI PciI PmeI PmlI PsiI PspXI PsrI PvuI
RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XhoI