Restriction map for
pFN21AE4562
(Linear) (Six-base) MAPSORT of: KIEE4562.seq Check: 3825 from: 1 to: 2067
KIEE4562.orf
With 182 enzymes: SgfI *
March 22, 2012 18:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1077 1795 2067
Size: 1077 718 272
Fragments arranged by size:
1077 718 272
Acc65I G'GTAC_C
Cuts at: 0 419 1832 2067
Size: 419 1413 235
Fragments arranged by size:
1413 419 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 967 2067
Size: 703 264 1100
Fragments arranged by size:
1100 703 264
AflIII A'CryG_T
Cuts at: 0 173 350 1862 2067
Size: 173 177 1512 205
Fragments arranged by size:
1512 205 177 173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 66 944 1606 2044 2067
Size: 66 878 662 438 23
Fragments arranged by size:
878 662 438 66 23
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1166 1521 1689 1928 2067
Size: 1062 104 355 168 239 139
Fragments arranged by size:
1062 355 239 168 139 104
ApaI G_GGCC'C
Cuts at: 0 792 2067
Size: 792 1275
AvaI C'yCGr_G
Cuts at: 0 406 598 1209 1595 2050 2067
Size: 406 192 611 386 455 17
Fragments arranged by size:
611 455 406 386 192 17
BanI G'GyrC_C
Cuts at: 0 154 419 1156 1506 1832 2067
Size: 154 265 737 350 326 235
Fragments arranged by size:
737 350 326 265 235 154
BanII G_rGCy'C
Cuts at: 0 119 596 792 1127 1604 2051 2067
Size: 119 477 196 335 477 447 16
Fragments arranged by size:
477 477 447 335 196 119 16
BbeI G_GCGC'C
Cuts at: 0 1160 1510 2067
Size: 1160 350 557
Fragments arranged by size:
1160 557 350
BbvCI CC'TCA_GC
Cuts at: 0 1972 2067
Size: 1972 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 546 580 1708 1742 2067
Size: 546 34 1128 34 325
Fragments arranged by size:
1128 546 325 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1336 1345 2067
Size: 1336 9 722
Fragments arranged by size:
1336 722 9
BlpI GC'TnA_GC
Cuts at: 0 511 2067
Size: 511 1556
Bme1580I G_kGCm'C
Cuts at: 0 90 792 1271 1595 2067
Size: 90 702 479 324 472
Fragments arranged by size:
702 479 472 324 90
BmgBI CAC'GTC
Cuts at: 0 174 327 351 1179 2067
Size: 174 153 24 828 888
Fragments arranged by size:
888 828 174 153 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1539 1815 2067
Size: 1539 276 252
Fragments arranged by size:
1539 276 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 445 562 640 859 961 1543 1627
Size: 358 87 117 78 219 102 582 84
Cuts at: 1627 2067
Size: 440
Fragments arranged by size:
582 440 358 219 117 102 87 84
78
Bpu10I CC'TnA_GC
Cuts at: 0 1972 2017 2067
Size: 1972 45 50
Fragments arranged by size:
1972 50 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 640 751 1051 2067
Size: 178 462 111 300 1016
Fragments arranged by size:
1016 462 300 178 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 1399 1932 2067
Size: 697 702 533 135
Fragments arranged by size:
702 697 533 135
BsaAI yAC'GTr
Cuts at: 0 696 1431 1485 2067
Size: 696 735 54 582
Fragments arranged by size:
735 696 582 54
BsaBI GATnn'nnATC
Cuts at: 0 1330 2067
Size: 1330 737
BsaHI Gr'CG_yC
Cuts at: 0 1157 1507 1817 2006 2067
Size: 1157 350 310 189 61
Fragments arranged by size:
1157 350 310 189 61
BsaWI w'CCGG_w
Cuts at: 0 281 2067
Size: 281 1786
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 1495 1525 2067
Size: 337 30 1128 30 542
Fragments arranged by size:
1128 542 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 736 763 1048 1165 1735 2067
Size: 115 621 27 285 117 570 332
Fragments arranged by size:
621 570 332 285 117 115 27
BseYI C'CCAG_C
Cuts at: 0 89 254 441 807 1107 2067
Size: 89 165 187 366 300 960
Fragments arranged by size:
960 366 300 187 165 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1105 1675 2067
Size: 41 1064 570 392
Fragments arranged by size:
1064 570 392 41
BsiEI CG_ry'CG
Cuts at: 0 990 2067
Size: 990 1077
BsiHKAI G_wGCw'C
Cuts at: 0 55 119 190 1127 1604 2067
Size: 55 64 71 937 477 463
Fragments arranged by size:
937 477 463 71 64 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1030 2067
Size: 1030 1037
Bsp1286I G_dGCh'C
Cuts at: 0 55 90 119 190 596 792 1127 1271
Size: 55 35 29 71 406 196 335 144
Cuts at: 1271 1595 1604 2051 2067
Size: 324 9 447 16
Fragments arranged by size:
447 406 335 324 196 144 71 55
35 29 16 9
BspEI T'CCGG_A
Cuts at: 0 281 2067
Size: 281 1786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 1070 1077 1661 1795 2022 2049 2067
Size: 77 993 7 584 134 227 27 18
Fragments arranged by size:
993 584 227 134 77 27 18 7
BsrBI CCG'CTC
Cuts at: 0 429 1491 2067
Size: 429 1062 576
Fragments arranged by size:
1062 576 429
BsrFI r'CCGG_y
Cuts at: 0 737 2067
Size: 737 1330
BssSI C'ACGA_G
Cuts at: 0 1147 2067
Size: 1147 920
BstAPI GCAn_nnn'nTGC
Cuts at: 0 19 1104 2067
Size: 19 1085 963
Fragments arranged by size:
1085 963 19
BstEII G'GTnAC_C
Cuts at: 0 216 648 2067
Size: 216 432 1419
Fragments arranged by size:
1419 432 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1423 2067
Size: 1423 644
BstYI r'GATC_y
Cuts at: 0 1745 2067
Size: 1745 322
BtgI C'CryG_G
Cuts at: 0 869 1249 1340 1856 2067
Size: 869 380 91 516 211
Fragments arranged by size:
869 516 380 211 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1317 2067
Size: 1317 750
EaeI y'GGCC_r
Cuts at: 0 203 851 872 987 1337 1346 1425 1760
Size: 203 648 21 115 350 9 79 335
Cuts at: 1760 1794 2067
Size: 34 273
Fragments arranged by size:
648 350 335 273 203 115 79 34
21 9
EagI C'GGCC_G
Cuts at: 0 987 2067
Size: 987 1080
EarI CTCTTCn'nnn_
Cuts at: 0 1651 2067
Size: 1651 416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1471 2067
Size: 49 1422 596
Fragments arranged by size:
1422 596 49
EcoICRI GAG'CTC
Cuts at: 0 117 1125 1602 2067
Size: 117 1008 477 465
Fragments arranged by size:
1008 477 465 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 445 562 640 703 859 961 967
Size: 358 87 117 78 63 156 102 6
Cuts at: 967 1543 1627 2067
Size: 576 84 440
Fragments arranged by size:
576 440 358 156 117 102 87 84
78 63 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1066 1306 1672 2020 2067
Size: 547 519 240 366 348 47
Fragments arranged by size:
547 519 366 348 240 47
EcoO109I rG'GnC_Cy
Cuts at: 0 78 972 1478 1686 1755 2067
Size: 78 894 506 208 69 312
Fragments arranged by size:
894 506 312 208 78 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1092 2067
Size: 1092 975
HaeII r_GCGC'y
Cuts at: 0 202 969 1160 1510 1589 1967 1980 2067
Size: 202 767 191 350 79 378 13 87
Fragments arranged by size:
767 378 350 202 191 87 79 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 160 192 1192 1224 1363 1395
Size: 28 32 100 32 1000 32 139 32
Cuts at: 1395 1495 1527 2067
Size: 100 32 540
Fragments arranged by size:
1000 540 139 100 100 32 32 32
32 32 28
KasI G'GCGC_C
Cuts at: 0 1156 1506 2067
Size: 1156 350 561
Fragments arranged by size:
1156 561 350
KpnI G_GTAC'C
Cuts at: 0 423 1836 2067
Size: 423 1413 231
Fragments arranged by size:
1413 423 231
MscI TGG'CCA
Cuts at: 0 853 874 1348 1427 2067
Size: 853 21 474 79 640
Fragments arranged by size:
853 640 474 79 21
MslI CAynn'nnrTG
Cuts at: 0 48 1861 2067
Size: 48 1813 206
Fragments arranged by size:
1813 206 48
MspA1I CmG'CkG
Cuts at: 0 1251 1475 1551 2067
Size: 1251 224 76 516
Fragments arranged by size:
1251 516 224 76
NarI GG'CG_CC
Cuts at: 0 1157 1507 2067
Size: 1157 350 560
Fragments arranged by size:
1157 560 350
NcoI C'CATG_G
Cuts at: 0 1340 1856 2067
Size: 1340 516 211
Fragments arranged by size:
1340 516 211
NspI r_CATG'y
Cuts at: 0 262 1011 1782 1866 2067
Size: 262 749 771 84 201
Fragments arranged by size:
771 749 262 201 84
PasI CC'CwG_GG
Cuts at: 0 912 2067
Size: 912 1155
PciI A'CATG_T
Cuts at: 0 1862 2067
Size: 1862 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 870 1166 2067
Size: 870 296 901
Fragments arranged by size:
901 870 296
PfoI T'CCnGG_A
Cuts at: 0 374 578 836 1604 2067
Size: 374 204 258 768 463
Fragments arranged by size:
768 463 374 258 204
PmlI CAC'GTG
Cuts at: 0 696 1431 1485 2067
Size: 696 735 54 582
Fragments arranged by size:
735 696 582 54
PpuMI rG'GwC_Cy
Cuts at: 0 78 1478 1755 2067
Size: 78 1400 277 312
Fragments arranged by size:
1400 312 277 78
PspOMI G'GGCC_C
Cuts at: 0 788 2067
Size: 788 1279
PstI C_TGCA'G
Cuts at: 0 1292 1391 2033 2067
Size: 1292 99 642 34
Fragments arranged by size:
1292 642 99 34
SacI G_AGCT'C
Cuts at: 0 119 1127 1604 2067
Size: 119 1008 477 463
Fragments arranged by size:
1008 477 463 119
SacII CC_GC'GG
Cuts at: 0 1252 2067
Size: 1252 815
SanDI GG'GwC_CC
Cuts at: 0 1478 1755 2067
Size: 1478 277 312
Fragments arranged by size:
1478 312 277
ScaI AGT'ACT
Cuts at: 0 1075 1825 2067
Size: 1075 750 242
Fragments arranged by size:
1075 750 242
SexAI A'CCwGG_T
Cuts at: 0 146 1025 2067
Size: 146 879 1042
Fragments arranged by size:
1042 879 146
SfcI C'TryA_G
Cuts at: 0 1288 1387 2029 2067
Size: 1288 99 642 38
Fragments arranged by size:
1288 642 99 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1345 2067
Size: 1345 722
SfoI GGC'GCC
Cuts at: 0 1158 1508 2067
Size: 1158 350 559
Fragments arranged by size:
1158 559 350
SmaI CCC'GGG
Cuts at: 0 1597 2067
Size: 1597 470
SmlI C'TyrA_G
Cuts at: 0 193 655 766 1066 2067
Size: 193 462 111 300 1001
Fragments arranged by size:
1001 462 300 193 111
StuI AGG'CCT
Cuts at: 0 727 1643 2067
Size: 727 916 424
Fragments arranged by size:
916 727 424
StyI C'CwwG_G
Cuts at: 0 272 1340 1673 1749 1856 2067
Size: 272 1068 333 76 107 211
Fragments arranged by size:
1068 333 272 211 107 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1521 2067
Size: 172 1349 546
Fragments arranged by size:
1349 546 172
TatI w'GTAC_w
Cuts at: 0 1073 1823 2067
Size: 1073 750 244
Fragments arranged by size:
1073 750 244
Tth111I GACn'n_nGTC
Cuts at: 0 832 2067
Size: 832 1235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 867 1338 1747 1804 2067
Size: 714 153 471 409 57 263
Fragments arranged by size:
714 471 409 263 153 57
XmaI C'CCGG_G
Cuts at: 0 1595 2067
Size: 1595 472
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI KpnI MscI MslI MspA1I NarI NcoI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI
SacI SacII SanDI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BfrBI BglII BmtI BplI
BsiWI BsmI BspHI BsrDI BsrGI BssHII BstBI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SapI SbfI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI XmnI ZraI