Restriction map for
pFN21AE4560
(Linear) (Six-base) MAPSORT of: KIEE4560.seq Check: 3998 from: 1 to: 2055
KIEE4560.orf
With 182 enzymes: SgfI *
March 22, 2012 18:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1050 2055
Size: 1050 1005
AccI GT'mk_AC
Cuts at: 0 1668 2044 2055
Size: 1668 376 11
Fragments arranged by size:
1668 376 11
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 151 1169 1580 2055
Size: 26 125 1018 411 475
Fragments arranged by size:
1018 475 411 125 26
AleI CACnn'nnGTG
Cuts at: 0 647 1060 2055
Size: 647 413 995
Fragments arranged by size:
995 647 413
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 2055
Size: 202 32 1821
Fragments arranged by size:
1821 202 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1703 2055
Size: 1703 352
ApaI G_GGCC'C
Cuts at: 0 365 2055
Size: 365 1690
ApoI r'AATT_y
Cuts at: 0 855 893 949 1791 2055
Size: 855 38 56 842 264
Fragments arranged by size:
855 842 264 56 38
AseI AT'TA_AT
Cuts at: 0 619 1499 2055
Size: 619 880 556
Fragments arranged by size:
880 619 556
AvaI C'yCGr_G
Cuts at: 0 254 2055
Size: 254 1801
BamHI G'GATC_C
Cuts at: 0 105 2055
Size: 105 1950
BanII G_rGCy'C
Cuts at: 0 170 365 1124 2055
Size: 170 195 759 931
Fragments arranged by size:
931 759 195 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 2055
Size: 89 1966
BbvCI CC'TCA_GC
Cuts at: 0 1237 2055
Size: 1237 818
BglII A'GATC_T
Cuts at: 0 374 420 2055
Size: 374 46 1635
Fragments arranged by size:
1635 374 46
Bme1580I G_kGCm'C
Cuts at: 0 365 2055
Size: 365 1690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1951 2055
Size: 1951 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 768 2055
Size: 112 656 1287
Fragments arranged by size:
1287 656 112
Bpu10I CC'TnA_GC
Cuts at: 0 1237 2055
Size: 1237 818
BsaHI Gr'CG_yC
Cuts at: 0 182 2055
Size: 182 1873
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 2055
Size: 202 30 1823
Fragments arranged by size:
1823 202 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1681 2055
Size: 1681 374
BsiHKAI G_wGCw'C
Cuts at: 0 299 2055
Size: 299 1756
BsmI GAATG_Cn'
Cuts at: 0 1073 2055
Size: 1073 982
Bsp1286I G_dGCh'C
Cuts at: 0 170 299 365 1124 2055
Size: 170 129 66 759 931
Fragments arranged by size:
931 759 170 129 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1050 2055
Size: 1050 1005
BsrDI GCAATG_nn'
Cuts at: 0 320 2055
Size: 320 1735
BsrGI T'GTAC_A
Cuts at: 0 1742 2055
Size: 1742 313
BssSI C'ACGA_G
Cuts at: 0 645 2049 2055
Size: 645 1404 6
Fragments arranged by size:
1404 645 6
BstEII G'GTnAC_C
Cuts at: 0 1577 2055
Size: 1577 478
BstXI CCAn_nnnn'nTGG
Cuts at: 0 229 736 1062 1529 2055
Size: 229 507 326 467 526
Fragments arranged by size:
526 507 467 326 229
BstYI r'GATC_y
Cuts at: 0 105 260 374 420 983 2055
Size: 105 155 114 46 563 1072
Fragments arranged by size:
1072 563 155 114 105 46
BstZ17I GTA'TAC
Cuts at: 0 2045 2055
Size: 2045 10
BtgI C'CryG_G
Cuts at: 0 1100 2055
Size: 1100 955
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 285 1954 2055
Size: 285 1669 101
Fragments arranged by size:
1669 285 101
BtsI GCAGTG_nn'
Cuts at: 0 1534 2055
Size: 1534 521
ClaI AT'CG_AT
Cuts at: 0 626 2055
Size: 626 1429
DraI TTT'AAA
Cuts at: 0 464 614 854 2055
Size: 464 150 240 1201
Fragments arranged by size:
1201 464 240 150
DraIII CAC_nnn'GTG
Cuts at: 0 1605 2055
Size: 1605 450
EaeI y'GGCC_r
Cuts at: 0 231 305 2055
Size: 231 74 1750
Fragments arranged by size:
1750 231 74
EarI CTCTTCn'nnn_
Cuts at: 0 271 2055
Size: 271 1784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 112 151 768 1169 1580 2055
Size: 26 86 39 617 401 411 475
Fragments arranged by size:
617 475 411 401 86 39 26
EcoO109I rG'GnC_Cy
Cuts at: 0 361 2055
Size: 361 1694
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 90 122 968 1000 2055
Size: 14 32 44 32 846 32 1055
Fragments arranged by size:
1055 846 44 32 32 32 14
HincII GTy'rAC
Cuts at: 0 1386 2055
Size: 1386 669
MfeI C'AATT_G
Cuts at: 0 670 2055
Size: 670 1385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 2055
Size: 658 1397
MscI TGG'CCA
Cuts at: 0 233 2055
Size: 233 1822
MslI CAynn'nnrTG
Cuts at: 0 34 647 734 1060 1679 2055
Size: 34 613 87 326 619 376
Fragments arranged by size:
619 613 376 326 87 34
NcoI C'CATG_G
Cuts at: 0 1100 2055
Size: 1100 955
NspI r_CATG'y
Cuts at: 0 1245 1305 1401 2018 2055
Size: 1245 60 96 617 37
Fragments arranged by size:
1245 617 96 60 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 761 2055
Size: 761 1294
PfoI T'CCnGG_A
Cuts at: 0 539 1007 1288 2055
Size: 539 468 281 767
Fragments arranged by size:
767 539 468 281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 2055
Size: 202 32 1821
Fragments arranged by size:
1821 202 32
PsiI TTA'TAA
Cuts at: 0 497 2055
Size: 497 1558
PspOMI G'GGCC_C
Cuts at: 0 361 2055
Size: 361 1694
SexAI A'CCwGG_T
Cuts at: 0 1573 2055
Size: 1573 482
SphI G_CATG'C
Cuts at: 0 1245 2018 2055
Size: 1245 773 37
Fragments arranged by size:
1245 773 37
SspI AAT'ATT
Cuts at: 0 1464 2055
Size: 1464 591
StyI C'CwwG_G
Cuts at: 0 153 206 382 1100 2055
Size: 153 53 176 718 955
Fragments arranged by size:
955 718 176 153 53
SwaI ATTT'AAAT
Cuts at: 0 854 2055
Size: 854 1201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 768 2055
Size: 768 1287
TatI w'GTAC_w
Cuts at: 0 1184 1742 2028 2055
Size: 1184 558 286 27
Fragments arranged by size:
1184 558 286 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1320 1352 2055
Size: 1320 32 703
Fragments arranged by size:
1320 703 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 1078 2055
Size: 1015 63 977
Fragments arranged by size:
1015 977 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 758 1059 1313 2055
Size: 758 301 254 742
Fragments arranged by size:
758 742 301 254
XmnI GAAnn'nnTTC
Cuts at: 0 86 2055
Size: 86 1969
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApoI
AseI AvaI BamHI BanII BbsI BbvCI BglII Bme1580I
BplI BpmI Bpu10I BsaHI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BssSI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EarI Eco57MI EcoO109I Hin4I HincII MfeI MmeI MscI
MslI NcoI NspI PflMI PfoI PpiI PsiI PspOMI
SexAI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI AscI AsiSI AvrII BaeI BanI BbeI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BstAPI BstBI Bsu36I CspCI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpuMI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI XbaI XhoI XmaI
ZraI