Restriction map for
pFN21AE4558
(Linear) (Six-base) MAPSORT of: KIEE4558.seq Check: 7494 from: 1 to: 2049
KIEE4558.orf
With 182 enzymes: SgfI *
March 22, 2012 18:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 676 714 1259 1534 1624 2049
Size: 637 39 38 545 275 90 425
Fragments arranged by size:
637 545 425 275 90 39 38
AflIII A'CryG_T
Cuts at: 0 458 1496 2049
Size: 458 1038 553
Fragments arranged by size:
1038 553 458
AhdI GACnn_n'nnGTC
Cuts at: 0 1385 2049
Size: 1385 664
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 2049
Size: 1742 32 275
Fragments arranged by size:
1742 275 32
ApaI G_GGCC'C
Cuts at: 0 275 2049
Size: 275 1774
ApoI r'AATT_y
Cuts at: 0 1144 1709 2049
Size: 1144 565 340
Fragments arranged by size:
1144 565 340
AvaI C'yCGr_G
Cuts at: 0 609 2049
Size: 609 1440
AvrII C'CTAG_G
Cuts at: 0 714 2049
Size: 714 1335
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1481 1514 2049
Size: 1481 33 535
Fragments arranged by size:
1481 535 33
BanI G'GyrC_C
Cuts at: 0 1050 1398 2049
Size: 1050 348 651
Fragments arranged by size:
1050 651 348
BanII G_rGCy'C
Cuts at: 0 275 884 1813 1961 2049
Size: 275 609 929 148 88
Fragments arranged by size:
929 609 275 148 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 1027 1534 1868 2049
Size: 764 263 507 334 181
Fragments arranged by size:
764 507 334 263 181
BciVI GTATCCnnnnn_n'
Cuts at: 0 1501 2049
Size: 1501 548
BclI T'GATC_A
Cuts at: 0 92 623 2049
Size: 92 531 1426
Fragments arranged by size:
1426 531 92
BglII A'GATC_T
Cuts at: 0 686 2049
Size: 686 1363
Bme1580I G_kGCm'C
Cuts at: 0 275 1403 2049
Size: 275 1128 646
Fragments arranged by size:
1128 646 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1274 2049
Size: 1274 775
Bpu10I CC'TnA_GC
Cuts at: 0 763 2049
Size: 763 1286
BsaAI yAC'GTr
Cuts at: 0 461 1419 2049
Size: 461 958 630
Fragments arranged by size:
958 630 461
BsaBI GATnn'nnATC
Cuts at: 0 55 315 2049
Size: 55 260 1734
Fragments arranged by size:
1734 260 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 959 989 1742 1772 2049
Size: 959 30 753 30 277
Fragments arranged by size:
959 753 277 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 2003 2049
Size: 173 1830 46
Fragments arranged by size:
1830 173 46
BsiEI CG_ry'CG
Cuts at: 0 1617 2049
Size: 1617 432
BsiHKAI G_wGCw'C
Cuts at: 0 165 728 2039 2049
Size: 165 563 1311 10
Fragments arranged by size:
1311 563 165 10
BsmI GAATG_Cn'
Cuts at: 0 1345 2049
Size: 1345 704
Bsp1286I G_dGCh'C
Cuts at: 0 165 275 728 884 1403 1813 1961 2039
Size: 165 110 453 156 519 410 148 78
Cuts at: 2039 2049
Size: 10
Fragments arranged by size:
519 453 410 165 156 148 110 78
10
BspHI T'CATG_A
Cuts at: 0 1280 2049
Size: 1280 769
BsrDI GCAATG_nn'
Cuts at: 0 1307 1655 2049
Size: 1307 348 394
Fragments arranged by size:
1307 394 348
BsrGI T'GTAC_A
Cuts at: 0 942 956 1354 1716 2049
Size: 942 14 398 362 333
Fragments arranged by size:
942 398 362 333 14
BstEII G'GTnAC_C
Cuts at: 0 796 2049
Size: 796 1253
BstYI r'GATC_y
Cuts at: 0 686 1070 1211 2049
Size: 686 384 141 838
Fragments arranged by size:
838 686 384 141
BtgI C'CryG_G
Cuts at: 0 542 1046 2049
Size: 542 504 1003
Fragments arranged by size:
1003 542 504
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 766 2049
Size: 400 366 1283
Fragments arranged by size:
1283 400 366
EaeI y'GGCC_r
Cuts at: 0 899 2049
Size: 899 1150
EarI CTCTTCn'nnn_
Cuts at: 0 125 667 2049
Size: 125 542 1382
Fragments arranged by size:
1382 542 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1244 2049
Size: 1244 805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 676 714 1259 1274 1534 1624 2049
Size: 637 39 38 545 15 260 90 425
Fragments arranged by size:
637 545 425 260 90 39 38 15
EcoO109I rG'GnC_Cy
Cuts at: 0 272 1518 2049
Size: 272 1246 531
Fragments arranged by size:
1246 531 272
EcoRV GAT'ATC
Cuts at: 0 537 2049
Size: 537 1512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1122 1134 1218 1830 2049
Size: 1122 12 84 612 219
Fragments arranged by size:
1122 612 219 84 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 676 708 807 839 1366 1371
Size: 62 32 582 32 99 32 527 5
Cuts at: 1371 1398 1403 2049
Size: 27 5 646
Fragments arranged by size:
646 582 527 99 62 32 32 32
27 5 5
HincII GTy'rAC
Cuts at: 0 525 1696 2049
Size: 525 1171 353
Fragments arranged by size:
1171 525 353
HindIII A'AGCT_T
Cuts at: 0 1322 1795 2049
Size: 1322 473 254
Fragments arranged by size:
1322 473 254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 957 2049
Size: 61 896 1092
Fragments arranged by size:
1092 896 61
MscI TGG'CCA
Cuts at: 0 901 2049
Size: 901 1148
MslI CAynn'nnrTG
Cuts at: 0 631 875 2049
Size: 631 244 1174
Fragments arranged by size:
1174 631 244
MspA1I CmG'CkG
Cuts at: 0 962 1048 2049
Size: 962 86 1001
Fragments arranged by size:
1001 962 86
NcoI C'CATG_G
Cuts at: 0 542 2049
Size: 542 1507
NdeI CA'TA_TG
Cuts at: 0 926 2049
Size: 926 1123
NspI r_CATG'y
Cuts at: 0 874 2049
Size: 874 1175
PfoI T'CCnGG_A
Cuts at: 0 1214 2042 2049
Size: 1214 828 7
Fragments arranged by size:
1214 828 7
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 2049
Size: 1742 32 275
Fragments arranged by size:
1742 275 32
PpuMI rG'GwC_Cy
Cuts at: 0 1518 2049
Size: 1518 531
PshAI GACnn'nnGTC
Cuts at: 0 1577 2049
Size: 1577 472
PsiI TTA'TAA
Cuts at: 0 380 2049
Size: 380 1669
PspOMI G'GGCC_C
Cuts at: 0 271 2049
Size: 271 1778
PvuII CAG'CTG
Cuts at: 0 962 2049
Size: 962 1087
SacII CC_GC'GG
Cuts at: 0 1049 2049
Size: 1049 1000
SfcI C'TryA_G
Cuts at: 0 306 2049
Size: 306 1743
SphI G_CATG'C
Cuts at: 0 874 2049
Size: 874 1175
SspI AAT'ATT
Cuts at: 0 831 2049
Size: 831 1218
StyI C'CwwG_G
Cuts at: 0 494 542 714 987 1644 2049
Size: 494 48 172 273 657 405
Fragments arranged by size:
657 494 405 273 172 48
TatI w'GTAC_w
Cuts at: 0 942 956 1354 1716 1773 2049
Size: 942 14 398 362 57 276
Fragments arranged by size:
942 398 362 276 57 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 780 812 1026 1058 1298 1330
Size: 242 32 506 32 214 32 240 32
Cuts at: 1330 1872 1904 2049
Size: 542 32 145
Fragments arranged by size:
542 506 242 240 214 145 32 32
32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 601 1114 1630 2049
Size: 601 513 516 419
Fragments arranged by size:
601 516 513 419
Enzymes that do cut:
AcuI AflIII AhdI AloI ApaI ApoI AvaI AvrII
BaeI BanI BanII BbsI BciVI BclI BglII Bme1580I
BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstEII BstYI BtgI
DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
NdeI NspI PfoI PpiI PpuMI PshAI PsiI PspOMI
PvuII SacII SfcI SphI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AlwNI ApaLI AscI AseI AsiSI BamHI
BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaHI BsaWI BseYI BsgI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI