Restriction map for
pFN21AE4556
(Linear) (Six-base) MAPSORT of: KIEE4556.seq Check: 9333 from: 1 to: 2031
KIEE4556.orf
With 182 enzymes: SgfI *
March 22, 2012 18:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 2031
Size: 229 1802
AfeI AGC'GCT
Cuts at: 0 616 2031
Size: 616 1415
AflIII A'CryG_T
Cuts at: 0 917 1443 2031
Size: 917 526 588
Fragments arranged by size:
917 588 526
AleI CACnn'nnGTG
Cuts at: 0 715 2031
Size: 715 1316
ApaI G_GGCC'C
Cuts at: 0 58 371 696 1190 1620 1789 2031
Size: 58 313 325 494 430 169 242
Fragments arranged by size:
494 430 325 313 242 169 58
ApoI r'AATT_y
Cuts at: 0 802 2031
Size: 802 1229
AseI AT'TA_AT
Cuts at: 0 1748 2031
Size: 1748 283
AvaI C'yCGr_G
Cuts at: 0 364 1781 2031
Size: 364 1417 250
Fragments arranged by size:
1417 364 250
BamHI G'GATC_C
Cuts at: 0 1346 1682 2031
Size: 1346 336 349
Fragments arranged by size:
1346 349 336
BanI G'GyrC_C
Cuts at: 0 1869 2031
Size: 1869 162
BanII G_rGCy'C
Cuts at: 0 58 272 371 696 779 1190 1620 1789
Size: 58 214 99 325 83 411 430 169
Cuts at: 1789 2031
Size: 242
Fragments arranged by size:
430 411 325 242 214 169 99 83
58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 1016 2031
Size: 982 34 1015
Fragments arranged by size:
1015 982 34
BclI T'GATC_A
Cuts at: 0 791 1379 2031
Size: 791 588 652
Fragments arranged by size:
791 652 588
BglI GCCn_nnn'nGGC
Cuts at: 0 898 2031
Size: 898 1133
Bme1580I G_kGCm'C
Cuts at: 0 58 182 254 371 696 1190 1620 1789
Size: 58 124 72 117 325 494 430 169
Cuts at: 1789 1811 1872 2031
Size: 22 61 159
Fragments arranged by size:
494 430 325 169 159 124 117 72
61 58 22
BmgBI CAC'GTC
Cuts at: 0 1444 2031
Size: 1444 587
BmrI ACTGGGnnnn_n'
Cuts at: 0 837 1670 2031
Size: 837 833 361
Fragments arranged by size:
837 833 361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 1882 2031
Size: 1427 455 149
Fragments arranged by size:
1427 455 149
Bpu10I CC'TnA_GC
Cuts at: 0 757 2031
Size: 757 1274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1253 1384 2031
Size: 109 1144 131 647
Fragments arranged by size:
1144 647 131 109
BsaWI w'CCGG_w
Cuts at: 0 1253 2031
Size: 1253 778
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 2031
Size: 521 30 1480
Fragments arranged by size:
1480 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 435 505 2031
Size: 435 70 1526
Fragments arranged by size:
1526 435 70
BseYI C'CCAG_C
Cuts at: 0 223 339 1018 1284 1577 1811 2031
Size: 223 116 679 266 293 234 220
Fragments arranged by size:
679 293 266 234 223 220 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 568 2031
Size: 401 167 1463
Fragments arranged by size:
1463 401 167
BsiEI CG_ry'CG
Cuts at: 0 1323 2031
Size: 1323 708
BsiHKAI G_wGCw'C
Cuts at: 0 669 2031
Size: 669 1362
BsmI GAATG_Cn'
Cuts at: 0 161 2031
Size: 161 1870
Bsp1286I G_dGCh'C
Cuts at: 0 58 182 254 272 371 669 696 779
Size: 58 124 72 18 99 298 27 83
Cuts at: 779 1190 1620 1789 1811 1872 2031
Size: 411 430 169 22 61 159
Fragments arranged by size:
430 411 298 169 159 124 99 83
72 61 58 27 22 18
BspHI T'CATG_A
Cuts at: 0 264 333 2031
Size: 264 69 1698
Fragments arranged by size:
1698 264 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 540 605 2031
Size: 414 126 65 1426
Fragments arranged by size:
1426 414 126 65
BsrBI CCG'CTC
Cuts at: 0 443 2031
Size: 443 1588
BsrDI GCAATG_nn'
Cuts at: 0 81 2031
Size: 81 1950
BsrFI r'CCGG_y
Cuts at: 0 200 2031
Size: 200 1831
BsrGI T'GTAC_A
Cuts at: 0 914 2031
Size: 914 1117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1333 2031
Size: 1333 698
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1720 2031
Size: 1720 311
BstYI r'GATC_y
Cuts at: 0 1346 1682 2031
Size: 1346 336 349
Fragments arranged by size:
1346 349 336
Bsu36I CC'TnA_GG
Cuts at: 0 358 2031
Size: 358 1673
BtgI C'CryG_G
Cuts at: 0 713 894 2031
Size: 713 181 1137
Fragments arranged by size:
1137 713 181
BtsI GCAGTG_nn'
Cuts at: 0 869 1562 1887 2031
Size: 869 693 325 144
Fragments arranged by size:
869 693 325 144
DraIII CAC_nnn'GTG
Cuts at: 0 1012 2031
Size: 1012 1019
EaeI y'GGCC_r
Cuts at: 0 132 274 1596 1764 1774 2031
Size: 132 142 1322 168 10 257
Fragments arranged by size:
1322 257 168 142 132 10
EarI CTCTTCn'nnn_
Cuts at: 0 1302 2031
Size: 1302 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1427 1882 2031
Size: 229 1198 455 149
Fragments arranged by size:
1198 455 229 149
EcoO109I rG'GnC_Cy
Cuts at: 0 55 368 692 693 702 784 1023 1087
Size: 55 313 324 1 9 82 239 64
Cuts at: 1087 1785 1786 2031
Size: 698 1 245
Fragments arranged by size:
698 324 313 245 239 82 64 55
9 1 1
EcoRI G'AATT_C
Cuts at: 0 802 2031
Size: 802 1229
FspI TGC'GCA
Cuts at: 0 73 1561 2031
Size: 73 1488 470
Fragments arranged by size:
1488 470 73
HaeII r_GCGC'y
Cuts at: 0 618 2031
Size: 618 1413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 495 527 776 808 1935 1942
Size: 412 32 51 32 249 32 1127 7
Cuts at: 1942 1967 1974 2031
Size: 25 7 57
Fragments arranged by size:
1127 412 249 57 51 32 32 32
25 7 7
HincII GTy'rAC
Cuts at: 0 1796 2031
Size: 1796 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1655 2031
Size: 473 1182 376
Fragments arranged by size:
1182 473 376
MscI TGG'CCA
Cuts at: 0 134 276 1598 1776 2031
Size: 134 142 1322 178 255
Fragments arranged by size:
1322 255 178 142 134
MslI CAynn'nnrTG
Cuts at: 0 715 1604 1822 1849 2031
Size: 715 889 218 27 182
Fragments arranged by size:
889 715 218 182 27
MspA1I CmG'CkG
Cuts at: 0 469 2031
Size: 469 1562
NdeI CA'TA_TG
Cuts at: 0 1119 1329 2031
Size: 1119 210 702
Fragments arranged by size:
1119 702 210
NspI r_CATG'y
Cuts at: 0 921 2031
Size: 921 1110
PasI CC'CwG_GG
Cuts at: 0 1353 2031
Size: 1353 678
PciI A'CATG_T
Cuts at: 0 917 2031
Size: 917 1114
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 630 1930 2031
Size: 309 321 1300 101
Fragments arranged by size:
1300 321 309 101
PfoI T'CCnGG_A
Cuts at: 0 1719 2031
Size: 1719 312
PpuMI rG'GwC_Cy
Cuts at: 0 784 1023 1087 2031
Size: 784 239 64 944
Fragments arranged by size:
944 784 239 64
PsiI TTA'TAA
Cuts at: 0 541 2031
Size: 541 1490
PspOMI G'GGCC_C
Cuts at: 0 54 367 692 1186 1616 1785 2031
Size: 54 313 325 494 430 169 246
Fragments arranged by size:
494 430 325 313 246 169 54
PstI C_TGCA'G
Cuts at: 0 611 701 1879 2031
Size: 611 90 1178 152
Fragments arranged by size:
1178 611 152 90
PvuI CG_AT'CG
Cuts at: 0 1323 2031
Size: 1323 708
PvuII CAG'CTG
Cuts at: 0 469 2031
Size: 469 1562
SbfI CC_TGCA'GG
Cuts at: 0 701 2031
Size: 701 1330
SfcI C'TryA_G
Cuts at: 0 105 607 697 1875 2031
Size: 105 502 90 1178 156
Fragments arranged by size:
1178 502 156 105 90
SmaI CCC'GGG
Cuts at: 0 366 1783 2031
Size: 366 1417 248
Fragments arranged by size:
1417 366 248
SmlI C'TyrA_G
Cuts at: 0 124 1268 1363 2031
Size: 124 1144 95 668
Fragments arranged by size:
1144 668 124 95
SrfI GCCC'GGGC
Cuts at: 0 366 2031
Size: 366 1665
SspI AAT'ATT
Cuts at: 0 316 2031
Size: 316 1715
StyI C'CwwG_G
Cuts at: 0 1730 1761 2031
Size: 1730 31 270
Fragments arranged by size:
1730 270 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 871 2031
Size: 97 774 1160
Fragments arranged by size:
1160 774 97
TatI w'GTAC_w
Cuts at: 0 914 2031
Size: 914 1117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1612 1644 1849 1881 2031
Size: 1159 32 421 32 205 32 150
Fragments arranged by size:
1159 421 205 150 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1851 2031
Size: 1851 180
XmaI C'CCGG_G
Cuts at: 0 364 1781 2031
Size: 364 1417 250
Fragments arranged by size:
1417 364 250
XmnI GAAnn'nnTTC
Cuts at: 0 317 2031
Size: 317 1714
Enzymes that do cut:
AcuI AfeI AflIII AleI ApaI ApoI AseI AvaI
BamHI BanI BanII BcgI BclI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI
NspI PasI PciI PflMI PfoI PpuMI PsiI PspOMI
PstI PvuI PvuII SbfI SfcI SmaI SmlI SrfI
SspI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI
BbeI BbsI BbvCI BciVI BfrBI BglII BlpI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsiWI BsmBI BspEI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PspXI PsrI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI StuI SwaI
Tth111I XbaI XhoI ZraI