Restriction map for
pFN21AE4555
(Linear) (Six-base) MAPSORT of: KIEE4555.seq Check: 1740 from: 1 to: 2025
KIEE4555.orf
With 182 enzymes: SgfI *
March 22, 2012 18:21 ..
Acc65I G'GTAC_C
Cuts at: 0 625 2025
Size: 625 1400
AleI CACnn'nnGTG
Cuts at: 0 1033 2025
Size: 1033 992
ApoI r'AATT_y
Cuts at: 0 94 229 394 448 778 1252 1292 1957
Size: 94 135 165 54 330 474 40 665
Cuts at: 1957 2025
Size: 68
Fragments arranged by size:
665 474 330 165 135 94 68 54
40
AseI AT'TA_AT
Cuts at: 0 214 2025
Size: 214 1811
AvaI C'yCGr_G
Cuts at: 0 1397 1655 2025
Size: 1397 258 370
Fragments arranged by size:
1397 370 258
BanI G'GyrC_C
Cuts at: 0 625 724 1453 1579 2025
Size: 625 99 729 126 446
Fragments arranged by size:
729 625 446 126 99
BbvCI CC'TCA_GC
Cuts at: 0 917 2025
Size: 917 1108
BciVI GTATCCnnnnn_n'
Cuts at: 0 1049 2025
Size: 1049 976
BclI T'GATC_A
Cuts at: 0 1902 2025
Size: 1902 123
BglI GCCn_nnn'nGGC
Cuts at: 0 16 2025
Size: 16 2009
BglII A'GATC_T
Cuts at: 0 704 2025
Size: 704 1321
BlpI GC'TnA_GC
Cuts at: 0 44 2025
Size: 44 1981
Bme1580I G_kGCm'C
Cuts at: 0 261 2025
Size: 261 1764
BmrI ACTGGGnnnn_n'
Cuts at: 0 393 533 1767 2025
Size: 393 140 1234 258
Fragments arranged by size:
1234 393 258 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 641 1034 1322 1334 2025
Size: 499 142 393 288 12 691
Fragments arranged by size:
691 499 393 288 142 12
Bpu10I CC'TnA_GC
Cuts at: 0 858 917 2025
Size: 858 59 1108
Fragments arranged by size:
1108 858 59
BsaBI GATnn'nnATC
Cuts at: 0 216 2025
Size: 216 1809
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 872 1850 2025
Size: 769 103 978 175
Fragments arranged by size:
978 769 175 103
BsiEI CG_ry'CG
Cuts at: 0 8 2025
Size: 8 2017
BsiHKAI G_wGCw'C
Cuts at: 0 485 1410 1752 2025
Size: 485 925 342 273
Fragments arranged by size:
925 485 342 273
BsmI GAATG_Cn'
Cuts at: 0 1070 2025
Size: 1070 955
Bsp1286I G_dGCh'C
Cuts at: 0 261 485 1410 1752 2025
Size: 261 224 925 342 273
Fragments arranged by size:
925 342 273 261 224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1539 1892 2025
Size: 1539 353 133
Fragments arranged by size:
1539 353 133
BssSI C'ACGA_G
Cuts at: 0 1751 2025
Size: 1751 274
BstEII G'GTnAC_C
Cuts at: 0 561 2025
Size: 561 1464
BstXI CCAn_nnnn'nTGG
Cuts at: 0 383 2025
Size: 383 1642
BstYI r'GATC_y
Cuts at: 0 36 704 1358 1442 2025
Size: 36 668 654 84 583
Fragments arranged by size:
668 654 583 84 36
Bsu36I CC'TnA_GG
Cuts at: 0 939 2025
Size: 939 1086
BtgI C'CryG_G
Cuts at: 0 797 1847 2025
Size: 797 1050 178
Fragments arranged by size:
1050 797 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1827 2025
Size: 1827 198
BtsI GCAGTG_nn'
Cuts at: 0 1028 2025
Size: 1028 997
ClaI AT'CG_AT
Cuts at: 0 1046 2025
Size: 1046 979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 152 187 1175 1210 2025
Size: 152 35 988 35 815
Fragments arranged by size:
988 815 152 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 559 2025
Size: 559 1466
EaeI y'GGCC_r
Cuts at: 0 5 1490 1662 2025
Size: 5 1485 172 363
Fragments arranged by size:
1485 363 172 5
EagI C'GGCC_G
Cuts at: 0 5 2025
Size: 5 2020
EarI CTCTTCn'nnn_
Cuts at: 0 884 1984 2025
Size: 884 1100 41
Fragments arranged by size:
1100 884 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 2025
Size: 559 1466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 641 1034 1322 1334 2025
Size: 499 142 393 288 12 691
Fragments arranged by size:
691 499 393 288 142 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1425 2025
Size: 1425 600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 1545 2025
Size: 513 1032 480
Fragments arranged by size:
1032 513 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 867 899 2025
Size: 21 32 814 32 1126
Fragments arranged by size:
1126 814 32 32 21
HincII GTy'rAC
Cuts at: 0 807 2025
Size: 807 1218
KpnI G_GTAC'C
Cuts at: 0 629 2025
Size: 629 1396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 2025
Size: 1002 1023
MscI TGG'CCA
Cuts at: 0 1664 2025
Size: 1664 361
MslI CAynn'nnrTG
Cuts at: 0 1033 1265 2025
Size: 1033 232 760
Fragments arranged by size:
1033 760 232
MspA1I CmG'CkG
Cuts at: 0 43 291 1679 2025
Size: 43 248 1388 346
Fragments arranged by size:
1388 346 248 43
NcoI C'CATG_G
Cuts at: 0 797 1847 2025
Size: 797 1050 178
Fragments arranged by size:
1050 797 178
NdeI CA'TA_TG
Cuts at: 0 441 2025
Size: 441 1584
NotI GC'GGCC_GC
Cuts at: 0 5 2025
Size: 5 2020
NspI r_CATG'y
Cuts at: 0 1466 2025
Size: 1466 559
PfoI T'CCnGG_A
Cuts at: 0 1873 2025
Size: 1873 152
PpuMI rG'GwC_Cy
Cuts at: 0 1425 2025
Size: 1425 600
PspXI vC'TCGA_Gb
Cuts at: 0 1397 2025
Size: 1397 628
PstI C_TGCA'G
Cuts at: 0 1602 2025
Size: 1602 423
PvuII CAG'CTG
Cuts at: 0 43 291 2025
Size: 43 248 1734
Fragments arranged by size:
1734 248 43
SfcI C'TryA_G
Cuts at: 0 708 987 1598 1807 2025
Size: 708 279 611 209 218
Fragments arranged by size:
708 611 279 218 209
SmaI CCC'GGG
Cuts at: 0 1657 2025
Size: 1657 368
SmlI C'TyrA_G
Cuts at: 0 1397 2025
Size: 1397 628
SphI G_CATG'C
Cuts at: 0 1466 2025
Size: 1466 559
SrfI GCCC'GGGC
Cuts at: 0 1657 2025
Size: 1657 368
SspI AAT'ATT
Cuts at: 0 59 2025
Size: 59 1966
StuI AGG'CCT
Cuts at: 0 937 2025
Size: 937 1088
StyI C'CwwG_G
Cuts at: 0 11 797 841 1847 2025
Size: 11 786 44 1006 178
Fragments arranged by size:
1006 786 178 44 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 810 1263 2025
Size: 810 453 762
Fragments arranged by size:
810 762 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1377 1409 2025
Size: 1377 32 616
Fragments arranged by size:
1377 616 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 2025
Size: 118 1907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1081 1730 2025
Size: 1081 649 295
Fragments arranged by size:
1081 649 295
XhoI C'TCGA_G
Cuts at: 0 1397 2025
Size: 1397 628
XmaI C'CCGG_G
Cuts at: 0 1655 2025
Size: 1655 370
Enzymes that do cut:
Acc65I AleI ApoI AseI AvaI BanI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BsaBI BseRI BsiEI BsiHKAI BsmI Bsp1286I BspMI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NdeI NotI NspI PfoI PpuMI PspXI PstI
PvuII SfcI SmaI SmlI SphI SrfI SspI StuI
StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BcgI
BfrBI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
DraI DraIII EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI
NarI NgoMIV NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SwaI TatI XbaI XmnI ZraI