Restriction map for
pFN21AE4554
(Linear) (Six-base) MAPSORT of: KIEE4554.seq Check: 4043 from: 1 to: 2007
KIEE4554.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 1231 1840 2007
Size: 1231 609 167
Fragments arranged by size:
1231 609 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 791 1045 2007
Size: 439 352 254 962
Fragments arranged by size:
962 439 352 254
AflII C'TTAA_G
Cuts at: 0 1189 2007
Size: 1189 818
AflIII A'CryG_T
Cuts at: 0 1812 2007
Size: 1812 195
ApoI r'AATT_y
Cuts at: 0 219 533 873 1733 1920 1966 2007
Size: 219 314 340 860 187 46 41
Fragments arranged by size:
860 340 314 219 187 46 41
BanI G'GyrC_C
Cuts at: 0 36 63 226 493 2007
Size: 36 27 163 267 1514
Fragments arranged by size:
1514 267 163 36 27
BanII G_rGCy'C
Cuts at: 0 182 293 708 2007
Size: 182 111 415 1299
Fragments arranged by size:
1299 415 182 111
BbeI G_GCGC'C
Cuts at: 0 67 497 2007
Size: 67 430 1510
Fragments arranged by size:
1510 430 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 758 779 1033 1520 2007
Size: 758 21 254 487 487
Fragments arranged by size:
758 487 487 254 21
BciVI GTATCCnnnnn_n'
Cuts at: 0 328 2007
Size: 328 1679
BclI T'GATC_A
Cuts at: 0 1476 2007
Size: 1476 531
BfrBI ATG'CAT
Cuts at: 0 303 1585 2007
Size: 303 1282 422
Fragments arranged by size:
1282 422 303
Bme1580I G_kGCm'C
Cuts at: 0 41 953 2007
Size: 41 912 1054
Fragments arranged by size:
1054 912 41
BmgBI CAC'GTC
Cuts at: 0 1813 2007
Size: 1813 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 758 2007
Size: 758 1249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 2007
Size: 666 1341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 2007
Size: 589 1418
Bpu10I CC'TnA_GC
Cuts at: 0 1080 2007
Size: 1080 927
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 1554 1651 2007
Size: 607 947 97 356
Fragments arranged by size:
947 607 356 97
BsaAI yAC'GTr
Cuts at: 0 81 957 1062 1571 2007
Size: 81 876 105 509 436
Fragments arranged by size:
876 509 436 105 81
BsaHI Gr'CG_yC
Cuts at: 0 64 494 2007
Size: 64 430 1513
Fragments arranged by size:
1513 430 64
BseYI C'CCAG_C
Cuts at: 0 102 420 812 1400 1937 2007
Size: 102 318 392 588 537 70
Fragments arranged by size:
588 537 392 318 102 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 2007
Size: 824 1183
BsiEI CG_ry'CG
Cuts at: 0 11 2007
Size: 11 1996
BsiHKAI G_wGCw'C
Cuts at: 0 86 2007
Size: 86 1921
BsiWI C'GTAC_G
Cuts at: 0 77 2007
Size: 77 1930
Bsp1286I G_dGCh'C
Cuts at: 0 41 86 182 293 708 953 2007
Size: 41 45 96 111 415 245 1054
Fragments arranged by size:
1054 415 245 111 96 45 41
BsrDI GCAATG_nn'
Cuts at: 0 1682 2007
Size: 1682 325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 736 2007
Size: 736 1271
BstYI r'GATC_y
Cuts at: 0 1195 1759 2007
Size: 1195 564 248
Fragments arranged by size:
1195 564 248
BstZ17I GTA'TAC
Cuts at: 0 1232 1841 2007
Size: 1232 609 166
Fragments arranged by size:
1232 609 166
BtgI C'CryG_G
Cuts at: 0 11 32 400 940 2007
Size: 11 21 368 540 1067
Fragments arranged by size:
1067 540 368 21 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1316 2007
Size: 1316 691
DraI TTT'AAA
Cuts at: 0 245 788 827 2007
Size: 245 543 39 1180
Fragments arranged by size:
1180 543 245 39
DraIII CAC_nnn'GTG
Cuts at: 0 957 2007
Size: 957 1050
EaeI y'GGCC_r
Cuts at: 0 8 1035 1138 2007
Size: 8 1027 103 869
Fragments arranged by size:
1027 869 103 8
EagI C'GGCC_G
Cuts at: 0 8 2007
Size: 8 1999
EarI CTCTTCn'nnn_
Cuts at: 0 361 685 1686 2007
Size: 361 324 1001 321
Fragments arranged by size:
1001 361 324 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 589 791 1045 2007
Size: 439 150 202 254 962
Fragments arranged by size:
962 439 254 202 150
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1667 2007
Size: 1667 340
HaeII r_GCGC'y
Cuts at: 0 67 497 2007
Size: 67 430 1510
Fragments arranged by size:
1510 430 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 2007
Size: 602 32 1373
Fragments arranged by size:
1373 602 32
HincII GTy'rAC
Cuts at: 0 746 1853 2007
Size: 746 1107 154
Fragments arranged by size:
1107 746 154
HindIII A'AGCT_T
Cuts at: 0 757 2007
Size: 757 1250
HpaI GTT'AAC
Cuts at: 0 1853 2007
Size: 1853 154
KasI G'GCGC_C
Cuts at: 0 63 493 2007
Size: 63 430 1514
Fragments arranged by size:
1514 430 63
MfeI C'AATT_G
Cuts at: 0 1946 2007
Size: 1946 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1613 2007
Size: 119 1494 394
Fragments arranged by size:
1494 394 119
MscI TGG'CCA
Cuts at: 0 1037 2007
Size: 1037 970
MslI CAynn'nnrTG
Cuts at: 0 1331 1915 2007
Size: 1331 584 92
Fragments arranged by size:
1331 584 92
MspA1I CmG'CkG
Cuts at: 0 13 34 137 459 1275 2007
Size: 13 21 103 322 816 732
Fragments arranged by size:
816 732 322 103 21 13
NarI GG'CG_CC
Cuts at: 0 64 494 2007
Size: 64 430 1513
Fragments arranged by size:
1513 430 64
NcoI C'CATG_G
Cuts at: 0 400 940 2007
Size: 400 540 1067
Fragments arranged by size:
1067 540 400
NotI GC'GGCC_GC
Cuts at: 0 8 2007
Size: 8 1999
NsiI A_TGCA'T
Cuts at: 0 305 1587 2007
Size: 305 1282 420
Fragments arranged by size:
1282 420 305
NspI r_CATG'y
Cuts at: 0 303 1884 2007
Size: 303 1581 123
Fragments arranged by size:
1581 303 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 513 946 1571 1933 2007
Size: 513 433 625 362 74
Fragments arranged by size:
625 513 433 362 74
PfoI T'CCnGG_A
Cuts at: 0 714 2007
Size: 714 1293
PvuII CAG'CTG
Cuts at: 0 459 1275 2007
Size: 459 816 732
Fragments arranged by size:
816 732 459
SacII CC_GC'GG
Cuts at: 0 14 35 2007
Size: 14 21 1972
Fragments arranged by size:
1972 21 14
SfcI C'TryA_G
Cuts at: 0 1219 2007
Size: 1219 788
SfoI GGC'GCC
Cuts at: 0 65 495 2007
Size: 65 430 1512
Fragments arranged by size:
1512 430 65
SmlI C'TyrA_G
Cuts at: 0 1189 2007
Size: 1189 818
SnaBI TAC'GTA
Cuts at: 0 1062 2007
Size: 1062 945
SphI G_CATG'C
Cuts at: 0 1884 2007
Size: 1884 123
SspI AAT'ATT
Cuts at: 0 381 1498 1509 2007
Size: 381 1117 11 498
Fragments arranged by size:
1117 498 381 11
StyI C'CwwG_G
Cuts at: 0 400 940 2007
Size: 400 540 1067
Fragments arranged by size:
1067 540 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 2007
Size: 790 1217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1934 2007
Size: 1934 73
XmnI GAAnn'nnTTC
Cuts at: 0 325 723 1259 2007
Size: 325 398 536 748
Fragments arranged by size:
748 536 398 325
Enzymes that do cut:
AccI AcuI AflII AflIII ApoI BanI BanII BbeI
BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BsrDI BstAPI BstYI BstZ17I BtgI
BtgZI DraI DraIII EaeI EagI EarI Eco57MI EcoNI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NotI NsiI NspI
PflMI PfoI PvuII SacII SfcI SfoI SmlI SnaBI
SphI SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BglI BglII
BlpI BmtI BpuEI BsaBI BsaWI BsaXI BseRI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI Bsu36I BtsI ClaI CspCI
DrdI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI KpnI MluI NaeI NdeI NgoMIV NheI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SpeI SrfI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI