Restriction map for
pFN21AE4553
(Linear) (Six-base) MAPSORT of: KIEE4553.seq Check: 6616 from: 1 to: 1995
KIEE4553.orf
With 182 enzymes: SgfI *
March 22, 2012 18:21 ..
AclI AA'CG_TT
Cuts at: 0 774 1995
Size: 774 1221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1672 1804 1866 1966 1995
Size: 35 1637 132 62 100 29
Fragments arranged by size:
1637 132 100 62 35 29
AfeI AGC'GCT
Cuts at: 0 724 1981 1995
Size: 724 1257 14
Fragments arranged by size:
1257 724 14
AflII C'TTAA_G
Cuts at: 0 1453 1995
Size: 1453 542
AflIII A'CryG_T
Cuts at: 0 1905 1995
Size: 1905 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1995
Size: 1157 838
AlwNI CAG_nnn'CTG
Cuts at: 0 73 391 1261 1995
Size: 73 318 870 734
Fragments arranged by size:
870 734 318 73
ApaI G_GGCC'C
Cuts at: 0 507 1374 1995
Size: 507 867 621
Fragments arranged by size:
867 621 507
AvaI C'yCGr_G
Cuts at: 0 1205 1802 1995
Size: 1205 597 193
Fragments arranged by size:
1205 597 193
BanI G'GyrC_C
Cuts at: 0 484 1763 1987 1995
Size: 484 1279 224 8
Fragments arranged by size:
1279 484 224 8
BanII G_rGCy'C
Cuts at: 0 450 507 1206 1237 1244 1374 1871 1995
Size: 450 57 699 31 7 130 497 124
Fragments arranged by size:
699 497 450 130 124 57 31 7
BbeI G_GCGC'C
Cuts at: 0 1767 1991 1995
Size: 1767 224 4
Fragments arranged by size:
1767 224 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 602 1423 1810 1995
Size: 403 199 821 387 185
Fragments arranged by size:
821 403 387 199 185
BbvCI CC'TCA_GC
Cuts at: 0 1330 1502 1715 1995
Size: 1330 172 213 280
Fragments arranged by size:
1330 280 213 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 581 615 1995
Size: 581 34 1380
Fragments arranged by size:
1380 581 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1027 1995
Size: 1027 968
BclI T'GATC_A
Cuts at: 0 1103 1995
Size: 1103 892
BglI GCCn_nnn'nGGC
Cuts at: 0 1498 1995
Size: 1498 497
BglII A'GATC_T
Cuts at: 0 533 1806 1995
Size: 533 1273 189
Fragments arranged by size:
1273 533 189
BlpI GC'TnA_GC
Cuts at: 0 88 1005 1294 1797 1995
Size: 88 917 289 503 198
Fragments arranged by size:
917 503 289 198 88
Bme1580I G_kGCm'C
Cuts at: 0 507 1374 1995
Size: 507 867 621
Fragments arranged by size:
867 621 507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 1995
Size: 311 1684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 255 1238 1339 1495 1699 1995
Size: 104 151 983 101 156 204 296
Fragments arranged by size:
983 296 204 156 151 104 101
Bpu10I CC'TnA_GC
Cuts at: 0 1238 1330 1502 1715 1995
Size: 1238 92 172 213 280
Fragments arranged by size:
1238 280 213 172 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 572 631 1969 1995
Size: 572 59 1338 26
Fragments arranged by size:
1338 572 59 26
BsaBI GATnn'nnATC
Cuts at: 0 1645 1995
Size: 1645 350
BsaHI Gr'CG_yC
Cuts at: 0 1764 1988 1995
Size: 1764 224 7
Fragments arranged by size:
1764 224 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 569 599 709 739 1051 1081 1995
Size: 569 30 110 30 312 30 914
Fragments arranged by size:
914 569 312 110 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 493 717 1237 1318 1720 1995
Size: 303 190 224 520 81 402 275
Fragments arranged by size:
520 402 303 275 224 190 81
BseYI C'CCAG_C
Cuts at: 0 391 411 826 1724 1844 1995
Size: 391 20 415 898 120 151
Fragments arranged by size:
898 415 391 151 120 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1995
Size: 343 1652
BsiHKAI G_wGCw'C
Cuts at: 0 749 1871 1995
Size: 749 1122 124
Fragments arranged by size:
1122 749 124
BsmI GAATG_Cn'
Cuts at: 0 363 1995
Size: 363 1632
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1038 1995
Size: 1038 957
Bsp1286I G_dGCh'C
Cuts at: 0 450 507 749 1206 1237 1244 1374 1871
Size: 450 57 242 457 31 7 130 497
Cuts at: 1871 1995
Size: 124
Fragments arranged by size:
497 457 450 242 130 124 57 31
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 304 1995
Size: 304 1691
BsrBI CCG'CTC
Cuts at: 0 666 1401 1995
Size: 666 735 594
Fragments arranged by size:
735 666 594
BsrDI GCAATG_nn'
Cuts at: 0 1031 1995
Size: 1031 964
BsrFI r'CCGG_y
Cuts at: 0 494 842 1972 1995
Size: 494 348 1130 23
Fragments arranged by size:
1130 494 348 23
BssSI C'ACGA_G
Cuts at: 0 947 1995
Size: 947 1048
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 675 1995
Size: 408 267 1320
Fragments arranged by size:
1320 408 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1488 1984 1995
Size: 1488 496 11
Fragments arranged by size:
1488 496 11
BstYI r'GATC_y
Cuts at: 0 533 1640 1779 1806 1995
Size: 533 1107 139 27 189
Fragments arranged by size:
1107 533 189 139 27
Bsu36I CC'TnA_GG
Cuts at: 0 334 1214 1703 1995
Size: 334 880 489 292
Fragments arranged by size:
880 489 334 292
BtgI C'CryG_G
Cuts at: 0 5 442 499 1244 1872 1995
Size: 5 437 57 745 628 123
Fragments arranged by size:
745 628 437 123 57 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1534 1995
Size: 1534 461
EaeI y'GGCC_r
Cuts at: 0 2 151 496 1310 1974 1995
Size: 2 149 345 814 664 21
Fragments arranged by size:
814 664 345 149 21 2
EarI CTCTTCn'nnn_
Cuts at: 0 1214 1421 1663 1995
Size: 1214 207 242 332
Fragments arranged by size:
1214 332 242 207
EcoICRI GAG'CTC
Cuts at: 0 1869 1995
Size: 1869 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 104 255 1238 1339 1495 1672 1699
Size: 35 69 151 983 101 156 177 27
Cuts at: 1699 1804 1866 1966 1995
Size: 105 62 100 29
Fragments arranged by size:
983 177 156 151 105 101 100 69
62 35 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 985 1579 1995
Size: 754 231 594 416
Fragments arranged by size:
754 594 416 231
EcoO109I rG'GnC_Cy
Cuts at: 0 504 861 939 1150 1210 1370 1371 1434
Size: 504 357 78 211 60 160 1 63
Cuts at: 1434 1896 1995
Size: 462 99
Fragments arranged by size:
504 462 357 211 160 99 78 63
60 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1195 1995
Size: 1195 800
FseI GG_CCGG'CC
Cuts at: 0 498 1995
Size: 498 1497
HaeII r_GCGC'y
Cuts at: 0 726 1326 1767 1983 1991 1995
Size: 726 600 441 216 8 4
Fragments arranged by size:
726 600 441 216 8 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 609 641 1051 1083 1995
Size: 199 32 378 32 410 32 912
Fragments arranged by size:
912 410 378 199 32 32 32
KasI G'GCGC_C
Cuts at: 0 1763 1987 1995
Size: 1763 224 8
Fragments arranged by size:
1763 224 8
MluI A'CGCG_T
Cuts at: 0 1905 1995
Size: 1905 90
MspA1I CmG'CkG
Cuts at: 0 7 55 172 212 444 768 1995
Size: 7 48 117 40 232 324 1227
Fragments arranged by size:
1227 324 232 117 48 40 7
NaeI GCC'GGC
Cuts at: 0 496 1974 1995
Size: 496 1478 21
Fragments arranged by size:
1478 496 21
NarI GG'CG_CC
Cuts at: 0 1764 1988 1995
Size: 1764 224 7
Fragments arranged by size:
1764 224 7
NcoI C'CATG_G
Cuts at: 0 499 1244 1995
Size: 499 745 751
Fragments arranged by size:
751 745 499
NgoMIV G'CCGG_C
Cuts at: 0 494 1972 1995
Size: 494 1478 23
Fragments arranged by size:
1478 494 23
PasI CC'CwG_GG
Cuts at: 0 1356 1995
Size: 1356 639
PfoI T'CCnGG_A
Cuts at: 0 560 1995
Size: 560 1435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1780 1812 1995
Size: 1780 32 183
Fragments arranged by size:
1780 183 32
PpuMI rG'GwC_Cy
Cuts at: 0 1150 1434 1896 1995
Size: 1150 284 462 99
Fragments arranged by size:
1150 462 284 99
PspOMI G'GGCC_C
Cuts at: 0 503 1370 1995
Size: 503 867 625
Fragments arranged by size:
867 625 503
PstI C_TGCA'G
Cuts at: 0 467 1136 1995
Size: 467 669 859
Fragments arranged by size:
859 669 467
PvuII CAG'CTG
Cuts at: 0 212 1995
Size: 212 1783
SacI G_AGCT'C
Cuts at: 0 1871 1995
Size: 1871 124
SacII CC_GC'GG
Cuts at: 0 8 445 1995
Size: 8 437 1550
Fragments arranged by size:
1550 437 8
ScaI AGT'ACT
Cuts at: 0 294 1995
Size: 294 1701
SexAI A'CCwGG_T
Cuts at: 0 581 1995
Size: 581 1414
SfcI C'TryA_G
Cuts at: 0 463 698 1132 1285 1600 1732 1995
Size: 463 235 434 153 315 132 263
Fragments arranged by size:
463 434 315 263 235 153 132
SfoI GGC'GCC
Cuts at: 0 1765 1989 1995
Size: 1765 224 6
Fragments arranged by size:
1765 224 6
SmlI C'TyrA_G
Cuts at: 0 1453 1995
Size: 1453 542
StyI C'CwwG_G
Cuts at: 0 499 1094 1145 1244 1995
Size: 499 595 51 99 751
Fragments arranged by size:
751 595 499 99 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 988 1995
Size: 988 1007
TatI w'GTAC_w
Cuts at: 0 292 1995
Size: 292 1703
Tth111I GACn'n_nGTC
Cuts at: 0 1063 1995
Size: 1063 932
XbaI T'CTAG_A
Cuts at: 0 219 1995
Size: 219 1776
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I
KasI MluI MspA1I NaeI NarI NcoI NgoMIV PasI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII
ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI
Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BfrBI BmgBI BmrI BmtI BpuEI BsaAI BsaWI BsiEI
BsiWI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI
MfeI MmeI MscI MslI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XcmI XhoI XmaI
XmnI ZraI