Restriction map for
pFN21AE4552
(Linear) (Six-base) MAPSORT of: KIEE4552.seq Check: 5953 from: 1 to: 1986
KIEE4552.orf
With 182 enzymes: SgfI *
March 22, 2012 18:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1232 1629 1986
Size: 1232 397 357
Fragments arranged by size:
1232 397 357
AatII G_ACGT'C
Cuts at: 0 1528 1986
Size: 1528 458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 529 559 1124 1456 1986
Size: 269 260 30 565 332 530
Fragments arranged by size:
565 530 332 269 260 30
AfeI AGC'GCT
Cuts at: 0 181 596 1986
Size: 181 415 1390
Fragments arranged by size:
1390 415 181
AhdI GACnn_n'nnGTC
Cuts at: 0 1116 1986
Size: 1116 870
AleI CACnn'nnGTG
Cuts at: 0 1651 1986
Size: 1651 335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 1986
Size: 916 1070
AlwNI CAG_nnn'CTG
Cuts at: 0 1602 1986
Size: 1602 384
ApaI G_GGCC'C
Cuts at: 0 1660 1986
Size: 1660 326
ApoI r'AATT_y
Cuts at: 0 220 296 1986
Size: 220 76 1690
Fragments arranged by size:
1690 220 76
AvaI C'yCGr_G
Cuts at: 0 434 1057 1689 1915 1986
Size: 434 623 632 226 71
Fragments arranged by size:
632 623 434 226 71
BanI G'GyrC_C
Cuts at: 0 19 1091 1573 1986
Size: 19 1072 482 413
Fragments arranged by size:
1072 482 413 19
BanII G_rGCy'C
Cuts at: 0 65 716 773 1660 1986
Size: 65 651 57 887 326
Fragments arranged by size:
887 651 326 65 57
BbvCI CC'TCA_GC
Cuts at: 0 1192 1986
Size: 1192 794
BciVI GTATCCnnnnn_n'
Cuts at: 0 268 379 1986
Size: 268 111 1607
Fragments arranged by size:
1607 268 111
BclI T'GATC_A
Cuts at: 0 990 1986
Size: 990 996
BglII A'GATC_T
Cuts at: 0 1809 1986
Size: 1809 177
Bme1580I G_kGCm'C
Cuts at: 0 1475 1576 1660 1986
Size: 1475 101 84 326
Fragments arranged by size:
1475 326 101 84
BmgBI CAC'GTC
Cuts at: 0 1470 1631 1835 1878 1986
Size: 1470 161 204 43 108
Fragments arranged by size:
1470 204 161 108 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 1486 1654 1986
Size: 1486 168 332
Fragments arranged by size:
1486 332 168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1131 1986
Size: 1131 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 694 1408 1986
Size: 677 17 714 578
Fragments arranged by size:
714 677 578 17
Bpu10I CC'TnA_GC
Cuts at: 0 1192 1986
Size: 1192 794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1326 1986
Size: 761 565 660
Fragments arranged by size:
761 660 565
BsaI GGTCTCn'nnnn_
Cuts at: 0 759 1046 1986
Size: 759 287 940
Fragments arranged by size:
940 759 287
BsaAI yAC'GTr
Cuts at: 0 1724 1986
Size: 1724 262
BsaBI GATnn'nnATC
Cuts at: 0 373 1986
Size: 373 1613
BsaHI Gr'CG_yC
Cuts at: 0 1525 1541 1986
Size: 1525 16 445
Fragments arranged by size:
1525 445 16
BsaWI w'CCGG_w
Cuts at: 0 452 1986
Size: 452 1534
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 721 751 1015 1045 1986
Size: 52 30 639 30 264 30 941
Fragments arranged by size:
941 639 264 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 1104 1246 1986
Size: 177 927 142 740
Fragments arranged by size:
927 740 177 142
BseYI C'CCAG_C
Cuts at: 0 176 597 814 948 1569 1986
Size: 176 421 217 134 621 417
Fragments arranged by size:
621 421 417 217 176 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1798 1986
Size: 529 1269 188
Fragments arranged by size:
1269 529 188
BsiEI CG_ry'CG
Cuts at: 0 17 1986
Size: 17 1969
BsiHKAI G_wGCw'C
Cuts at: 0 1682 1986
Size: 1682 304
BsmI GAATG_Cn'
Cuts at: 0 36 1986
Size: 36 1950
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 388 1825 1986
Size: 115 273 1437 161
Fragments arranged by size:
1437 273 161 115
Bsp1286I G_dGCh'C
Cuts at: 0 65 716 773 1475 1576 1660 1682 1986
Size: 65 651 57 702 101 84 22 304
Fragments arranged by size:
702 651 304 101 84 65 57 22
BspEI T'CCGG_A
Cuts at: 0 452 1986
Size: 452 1534
BspHI T'CATG_A
Cuts at: 0 1199 1986
Size: 1199 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 449 1232 1629 1986
Size: 137 312 783 397 357
Fragments arranged by size:
783 397 357 312 137
BsrBI CCG'CTC
Cuts at: 0 1015 1986
Size: 1015 971
BsrDI GCAATG_nn'
Cuts at: 0 1943 1986
Size: 1943 43
BsrFI r'CCGG_y
Cuts at: 0 1094 1986
Size: 1094 892
BstBI TT'CG_AA
Cuts at: 0 1769 1986
Size: 1769 217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 672 726 1461 1653 1986
Size: 108 564 54 735 192 333
Fragments arranged by size:
735 564 333 192 108 54
BstYI r'GATC_y
Cuts at: 0 1809 1986
Size: 1809 177
Bsu36I CC'TnA_GG
Cuts at: 0 1960 1986
Size: 1960 26
BtgI C'CryG_G
Cuts at: 0 1121 1460 1986
Size: 1121 339 526
Fragments arranged by size:
1121 526 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 651 1986
Size: 651 1335
EaeI y'GGCC_r
Cuts at: 0 2 329 662 1451 1643 1986
Size: 2 327 333 789 192 343
Fragments arranged by size:
789 343 333 327 192 2
EarI CTCTTCn'nnn_
Cuts at: 0 832 1100 1180 1330 1986
Size: 832 268 80 150 656
Fragments arranged by size:
832 656 268 150 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1927 1986
Size: 1927 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 529 559 677 694 1124 1408 1456
Size: 269 260 30 118 17 430 284 48
Cuts at: 1456 1986
Size: 530
Fragments arranged by size:
530 430 284 269 260 118 48 30
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 261 1755 1986
Size: 261 1494 231
Fragments arranged by size:
1494 261 231
EcoO109I rG'GnC_Cy
Cuts at: 0 228 253 340 438 1184 1986
Size: 228 25 87 98 746 802
Fragments arranged by size:
802 746 228 98 87 25
EcoRI G'AATT_C
Cuts at: 0 220 1986
Size: 220 1766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1203 1320 1986
Size: 1203 117 666
Fragments arranged by size:
1203 666 117
FspI TGC'GCA
Cuts at: 0 1228 1986
Size: 1228 758
HaeII r_GCGC'y
Cuts at: 0 183 598 660 737 821 1986
Size: 183 415 62 77 84 1165
Fragments arranged by size:
1165 415 183 84 77 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 82 84 114 721 753 958 990
Size: 52 30 2 30 607 32 205 32
Cuts at: 990 1102 1134 1986
Size: 112 32 852
Fragments arranged by size:
852 607 205 112 52 32 32 32
30 30 2
HindIII A'AGCT_T
Cuts at: 0 541 1986
Size: 541 1445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1562 1832 1986
Size: 1562 270 154
Fragments arranged by size:
1562 270 154
MscI TGG'CCA
Cuts at: 0 331 664 1453 1645 1986
Size: 331 333 789 192 341
Fragments arranged by size:
789 341 333 331 192
MslI CAynn'nnrTG
Cuts at: 0 25 367 523 1651 1675 1986
Size: 25 342 156 1128 24 311
Fragments arranged by size:
1128 342 311 156 25 24
MspA1I CmG'CkG
Cuts at: 0 208 722 1151 1986
Size: 208 514 429 835
Fragments arranged by size:
835 514 429 208
NcoI C'CATG_G
Cuts at: 0 1121 1460 1986
Size: 1121 339 526
Fragments arranged by size:
1121 526 339
NspI r_CATG'y
Cuts at: 0 489 1032 1986
Size: 489 543 954
Fragments arranged by size:
954 543 489
PasI CC'CwG_GG
Cuts at: 0 1602 1986
Size: 1602 384
PfoI T'CCnGG_A
Cuts at: 0 301 1986
Size: 301 1685
PpuMI rG'GwC_Cy
Cuts at: 0 228 253 340 438 1184 1986
Size: 228 25 87 98 746 802
Fragments arranged by size:
802 746 228 98 87 25
PspOMI G'GGCC_C
Cuts at: 0 1656 1986
Size: 1656 330
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1411 1443 1986
Size: 1411 32 543
Fragments arranged by size:
1411 543 32
PstI C_TGCA'G
Cuts at: 0 51 1507 1640 1986
Size: 51 1456 133 346
Fragments arranged by size:
1456 346 133 51
PvuII CAG'CTG
Cuts at: 0 208 1151 1986
Size: 208 943 835
Fragments arranged by size:
943 835 208
RsrII CG'GwC_CG
Cuts at: 0 626 1986
Size: 626 1360
SbfI CC_TGCA'GG
Cuts at: 0 1640 1986
Size: 1640 346
SfcI C'TryA_G
Cuts at: 0 47 1503 1636 1729 1986
Size: 47 1456 133 93 257
Fragments arranged by size:
1456 257 133 93 47
SmaI CCC'GGG
Cuts at: 0 436 1986
Size: 436 1550
SmlI C'TyrA_G
Cuts at: 0 776 1305 1986
Size: 776 529 681
Fragments arranged by size:
776 681 529
StuI AGG'CCT
Cuts at: 0 1441 1986
Size: 1441 545
StyI C'CwwG_G
Cuts at: 0 1121 1460 1986
Size: 1121 339 526
Fragments arranged by size:
1121 526 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 1200 1986
Size: 3 1197 786
Fragments arranged by size:
1197 786 3
TatI w'GTAC_w
Cuts at: 0 1271 1514 1986
Size: 1271 243 472
Fragments arranged by size:
1271 472 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 857 889 1015 1047 1986
Size: 857 32 126 32 939
Fragments arranged by size:
939 857 126 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1210 1986
Size: 1210 776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 1584 1896 1986
Size: 723 861 312 90
Fragments arranged by size:
861 723 312 90
XmaI C'CCGG_G
Cuts at: 0 434 1986
Size: 434 1552
ZraI GAC'GTC
Cuts at: 0 1526 1986
Size: 1526 460
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbvCI BciVI BclI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BstBI BstXI BstYI Bsu36I
BtgI BtgZI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NspI PasI PfoI PpuMI PspOMI
PsrI PstI PvuII RsrII SbfI SfcI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BfrBI BglI BlpI BmtI BsiWI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRV FseI FspAI HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmnI