Restriction map for
pFN21AE4550
(Linear) (Six-base) MAPSORT of: KIEE4550.seq Check: 2985 from: 1 to: 1971
KIEE4550.orf
With 182 enzymes: SgfI *
March 22, 2012 18:21 ..
AccI GT'mk_AC
Cuts at: 0 58 954 1971
Size: 58 896 1017
Fragments arranged by size:
1017 896 58
Acc65I G'GTAC_C
Cuts at: 0 117 1971
Size: 117 1854
AclI AA'CG_TT
Cuts at: 0 212 1971
Size: 212 1759
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 93 272 629 706 717 812 1172
Size: 6 87 179 357 77 11 95 360
Cuts at: 1172 1631 1684 1971
Size: 459 53 287
Fragments arranged by size:
459 360 357 287 179 95 87 77
53 11 6
AflIII A'CryG_T
Cuts at: 0 882 1971
Size: 882 1089
AleI CACnn'nnGTG
Cuts at: 0 343 1971
Size: 343 1628
AlwNI CAG_nnn'CTG
Cuts at: 0 1426 1848 1971
Size: 1426 422 123
Fragments arranged by size:
1426 422 123
ApoI r'AATT_y
Cuts at: 0 709 898 1009 1289 1393 1971
Size: 709 189 111 280 104 578
Fragments arranged by size:
709 578 280 189 111 104
AseI AT'TA_AT
Cuts at: 0 1901 1971
Size: 1901 70
AvaI C'yCGr_G
Cuts at: 0 1255 1971
Size: 1255 716
AvrII C'CTAG_G
Cuts at: 0 1266 1971
Size: 1266 705
BanI G'GyrC_C
Cuts at: 0 117 1971
Size: 117 1854
BanII G_rGCy'C
Cuts at: 0 1488 1971
Size: 1488 483
BbsI GAAGACnn'nnnn_
Cuts at: 0 301 453 1826 1971
Size: 301 152 1373 145
Fragments arranged by size:
1373 301 152 145
BlpI GC'TnA_GC
Cuts at: 0 1838 1971
Size: 1838 133
BmtI G_CTAG'C
Cuts at: 0 1588 1971
Size: 1588 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1529 1971
Size: 1529 442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 828 979 1971
Size: 766 62 151 992
Fragments arranged by size:
992 766 151 62
BsaAI yAC'GTr
Cuts at: 0 412 1971
Size: 412 1559
BsaHI Gr'CG_yC
Cuts at: 0 10 1971
Size: 10 1961
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 869 899 1971
Size: 869 30 1072
Fragments arranged by size:
1072 869 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 890 1321 1971
Size: 890 431 650
Fragments arranged by size:
890 650 431
BseYI C'CCAG_C
Cuts at: 0 391 1740 1971
Size: 391 1349 231
Fragments arranged by size:
1349 391 231
BsiHKAI G_wGCw'C
Cuts at: 0 679 1971
Size: 679 1292
Bsp1286I G_dGCh'C
Cuts at: 0 679 1488 1971
Size: 679 809 483
Fragments arranged by size:
809 679 483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 993 1971
Size: 993 978
BsrBI CCG'CTC
Cuts at: 0 18 1971
Size: 18 1953
BsrDI GCAATG_nn'
Cuts at: 0 426 754 1971
Size: 426 328 1217
Fragments arranged by size:
1217 426 328
BssSI C'ACGA_G
Cuts at: 0 1167 1971
Size: 1167 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 121 1740 1971
Size: 121 1619 231
Fragments arranged by size:
1619 231 121
BtsI GCAGTG_nn'
Cuts at: 0 132 1971
Size: 132 1839
DraI TTT'AAA
Cuts at: 0 1545 1971
Size: 1545 426
EaeI y'GGCC_r
Cuts at: 0 488 1971
Size: 488 1483
EarI CTCTTCn'nnn_
Cuts at: 0 25 1237 1971
Size: 25 1212 734
Fragments arranged by size:
1212 734 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 93 272 629 706 717 812 1172
Size: 6 87 179 357 77 11 95 360
Cuts at: 1172 1631 1684 1971
Size: 459 53 287
Fragments arranged by size:
459 360 357 287 179 95 87 77
53 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1706 1971
Size: 1706 265
EcoO109I rG'GnC_Cy
Cuts at: 0 1276 1971
Size: 1276 695
EcoRI G'AATT_C
Cuts at: 0 1393 1971
Size: 1393 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1749 1781 1971
Size: 1030 32 687 32 190
Fragments arranged by size:
1030 687 190 32 32
HincII GTy'rAC
Cuts at: 0 955 1313 1971
Size: 955 358 658
Fragments arranged by size:
955 658 358
HindIII A'AGCT_T
Cuts at: 0 158 1971
Size: 158 1813
HpaI GTT'AAC
Cuts at: 0 1313 1971
Size: 1313 658
KpnI G_GTAC'C
Cuts at: 0 121 1971
Size: 121 1850
MfeI C'AATT_G
Cuts at: 0 686 1971
Size: 686 1285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 691 1459 1971
Size: 31 660 768 512
Fragments arranged by size:
768 660 512 31
MscI TGG'CCA
Cuts at: 0 490 1971
Size: 490 1481
MslI CAynn'nnrTG
Cuts at: 0 343 1828 1971
Size: 343 1485 143
Fragments arranged by size:
1485 343 143
NheI G'CTAG_C
Cuts at: 0 1584 1971
Size: 1584 387
NspI r_CATG'y
Cuts at: 0 886 1380 1971
Size: 886 494 591
Fragments arranged by size:
886 591 494
PasI CC'CwG_GG
Cuts at: 0 591 1971
Size: 591 1380
PciI A'CATG_T
Cuts at: 0 882 1971
Size: 882 1089
PpuMI rG'GwC_Cy
Cuts at: 0 1276 1971
Size: 1276 695
PsiI TTA'TAA
Cuts at: 0 190 821 1971
Size: 190 631 1150
Fragments arranged by size:
1150 631 190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1200 1232 1971
Size: 1200 32 739
Fragments arranged by size:
1200 739 32
SalI G'TCGA_C
Cuts at: 0 953 1971
Size: 953 1018
SapI GCTCTTCn'nnn_
Cuts at: 0 25 1971
Size: 25 1946
SmlI C'TyrA_G
Cuts at: 0 781 843 994 1971
Size: 781 62 151 977
Fragments arranged by size:
977 781 151 62
SnaBI TAC'GTA
Cuts at: 0 412 1971
Size: 412 1559
SspI AAT'ATT
Cuts at: 0 335 1971
Size: 335 1636
StuI AGG'CCT
Cuts at: 0 70 841 1971
Size: 70 771 1130
Fragments arranged by size:
1130 771 70
StyI C'CwwG_G
Cuts at: 0 1266 1589 1971
Size: 1266 323 382
Fragments arranged by size:
1266 382 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1732 1971
Size: 1732 239
XbaI T'CTAG_A
Cuts at: 0 174 1971
Size: 174 1797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApoI
AseI AvaI AvrII BanI BanII BbsI BlpI BmtI
BplI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
HindIII HpaI KpnI MfeI MmeI MscI MslI NheI
NspI PasI PciI PpuMI PsiI PsrI SalI SapI
SmlI SnaBI SspI StuI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII KasI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI SwaI TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI