Restriction map for
pFN21AE4549
(Linear) (Six-base) MAPSORT of: KIEE4549.seq Check: 2620 from: 1 to: 1953
KIEE4549.orf
With 182 enzymes: SgfI *
March 22, 2012 18:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1272 1953
Size: 1272 681
AatII G_ACGT'C
Cuts at: 0 934 1953
Size: 934 1019
AccI GT'mk_AC
Cuts at: 0 410 1953
Size: 410 1543
Acc65I G'GTAC_C
Cuts at: 0 188 1953
Size: 188 1765
AclI AA'CG_TT
Cuts at: 0 1259 1953
Size: 1259 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 1953
Size: 1694 259
AflII C'TTAA_G
Cuts at: 0 838 1953
Size: 838 1115
AleI CACnn'nnGTG
Cuts at: 0 352 706 1953
Size: 352 354 1247
Fragments arranged by size:
1247 354 352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 938 1073 1953
Size: 938 135 880
Fragments arranged by size:
938 880 135
AlwNI CAG_nnn'CTG
Cuts at: 0 342 860 1953
Size: 342 518 1093
Fragments arranged by size:
1093 518 342
ApaI G_GGCC'C
Cuts at: 0 482 1953
Size: 482 1471
ApoI r'AATT_y
Cuts at: 0 1761 1953
Size: 1761 192
AvaI C'yCGr_G
Cuts at: 0 5 34 1953
Size: 5 29 1919
Fragments arranged by size:
1919 29 5
BamHI G'GATC_C
Cuts at: 0 974 1653 1953
Size: 974 679 300
Fragments arranged by size:
974 679 300
BanI G'GyrC_C
Cuts at: 0 188 843 1129 1535 1953
Size: 188 655 286 406 418
Fragments arranged by size:
655 418 406 286 188
BanII G_rGCy'C
Cuts at: 0 100 482 1479 1754 1953
Size: 100 382 997 275 199
Fragments arranged by size:
997 382 275 199 100
BbvCI CC'TCA_GC
Cuts at: 0 361 1953
Size: 361 1592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 871 905 1782 1816 1953
Size: 871 34 877 34 137
Fragments arranged by size:
877 871 137 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 593 1913 1953
Size: 593 1320 40
Fragments arranged by size:
1320 593 40
BglI GCCn_nnn'nGGC
Cuts at: 0 28 492 1953
Size: 28 464 1461
Fragments arranged by size:
1461 464 28
BglII A'GATC_T
Cuts at: 0 740 1953
Size: 740 1213
Bme1580I G_kGCm'C
Cuts at: 0 378 482 1538 1953
Size: 378 104 1056 415
Fragments arranged by size:
1056 415 378 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 830 946 1563 1953
Size: 830 116 617 390
Fragments arranged by size:
830 617 390 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 225 1561 1953
Size: 153 72 1336 392
Fragments arranged by size:
1336 392 153 72
Bpu10I CC'TnA_GC
Cuts at: 0 361 1953
Size: 361 1592
BsaAI yAC'GTr
Cuts at: 0 1740 1953
Size: 1740 213
BsaBI GATnn'nnATC
Cuts at: 0 1396 1953
Size: 1396 557
BsaHI Gr'CG_yC
Cuts at: 0 165 270 931 1953
Size: 165 105 661 1022
Fragments arranged by size:
1022 661 165 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 172 202 1953
Size: 85 30 57 30 1751
Fragments arranged by size:
1751 85 57 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 1953
Size: 193 1760
BseYI C'CCAG_C
Cuts at: 0 60 290 1803 1953
Size: 60 230 1513 150
Fragments arranged by size:
1513 230 150 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 934 1871 1953
Size: 908 26 937 82
Fragments arranged by size:
937 908 82 26
BsiEI CG_ry'CG
Cuts at: 0 199 1328 1953
Size: 199 1129 625
Fragments arranged by size:
1129 625 199
BsiHKAI G_wGCw'C
Cuts at: 0 1754 1953
Size: 1754 199
Bsp1286I G_dGCh'C
Cuts at: 0 100 378 482 1479 1538 1754 1953
Size: 100 278 104 997 59 216 199
Fragments arranged by size:
997 278 216 199 104 100 59
BspHI T'CATG_A
Cuts at: 0 1775 1953
Size: 1775 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 1121 1272 1953
Size: 350 771 151 681
Fragments arranged by size:
771 681 350 151
BsrFI r'CCGG_y
Cuts at: 0 191 1953
Size: 191 1762
BsrGI T'GTAC_A
Cuts at: 0 659 1953
Size: 659 1294
BssHII G'CGCG_C
Cuts at: 0 56 1953
Size: 56 1897
BstXI CCAn_nnnn'nTGG
Cuts at: 0 708 1416 1953
Size: 708 708 537
Fragments arranged by size:
708 708 537
BstYI r'GATC_y
Cuts at: 0 740 974 1653 1820 1953
Size: 740 234 679 167 133
Fragments arranged by size:
740 679 234 167 133
BtgI C'CryG_G
Cuts at: 0 221 1035 1322 1953
Size: 221 814 287 631
Fragments arranged by size:
814 631 287 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 342 1953
Size: 342 1611
BtsI GCAGTG_nn'
Cuts at: 0 190 378 1953
Size: 190 188 1575
Fragments arranged by size:
1575 190 188
ClaI AT'CG_AT
Cuts at: 0 683 1211 1953
Size: 683 528 742
Fragments arranged by size:
742 683 528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1554 1589 1953
Size: 1554 35 364
Fragments arranged by size:
1554 364 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 694 1785 1953
Size: 694 1091 168
Fragments arranged by size:
1091 694 168
EaeI y'GGCC_r
Cuts at: 0 196 865 1087 1343 1562 1953
Size: 196 669 222 256 219 391
Fragments arranged by size:
669 391 256 222 219 196
EagI C'GGCC_G
Cuts at: 0 196 1953
Size: 196 1757
EarI CTCTTCn'nnn_
Cuts at: 0 1670 1953
Size: 1670 283
EcoICRI GAG'CTC
Cuts at: 0 1752 1953
Size: 1752 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 225 1561 1694 1953
Size: 153 72 1336 133 259
Fragments arranged by size:
1336 259 153 133 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 1953
Size: 469 1484
EcoO109I rG'GnC_Cy
Cuts at: 0 214 339 478 479 824 940 1953
Size: 214 125 139 1 345 116 1013
Fragments arranged by size:
1013 345 214 139 125 116 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 612 799 1839 1953
Size: 612 187 1040 114
Fragments arranged by size:
1040 612 187 114
FspI TGC'GCA
Cuts at: 0 346 1953
Size: 346 1607
HaeII r_GCGC'y
Cuts at: 0 34 1240 1953
Size: 34 1206 713
Fragments arranged by size:
1206 713 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1342 1374 1812 1844 1953
Size: 85 32 1225 32 438 32 109
Fragments arranged by size:
1225 438 109 85 32 32 32
HincII GTy'rAC
Cuts at: 0 411 1044 1953
Size: 411 633 909
Fragments arranged by size:
909 633 411
KpnI G_GTAC'C
Cuts at: 0 192 1953
Size: 192 1761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1725 1953
Size: 752 973 228
Fragments arranged by size:
973 752 228
MscI TGG'CCA
Cuts at: 0 1345 1564 1953
Size: 1345 219 389
Fragments arranged by size:
1345 389 219
MslI CAynn'nnrTG
Cuts at: 0 352 383 706 1055 1282 1953
Size: 352 31 323 349 227 671
Fragments arranged by size:
671 352 349 323 227 31
MspA1I CmG'CkG
Cuts at: 0 201 645 918 1092 1324 1554 1560 1953
Size: 201 444 273 174 232 230 6 393
Fragments arranged by size:
444 393 273 232 230 201 174 6
NotI GC'GGCC_GC
Cuts at: 0 196 1953
Size: 196 1757
NspI r_CATG'y
Cuts at: 0 231 1281 1953
Size: 231 1050 672
Fragments arranged by size:
1050 672 231
PasI CC'CwG_GG
Cuts at: 0 828 1953
Size: 828 1125
PflMI CCAn_nnn'nTGG
Cuts at: 0 395 1953
Size: 395 1558
PpuMI rG'GwC_Cy
Cuts at: 0 339 824 1953
Size: 339 485 1129
Fragments arranged by size:
1129 485 339
PshAI GACnn'nnGTC
Cuts at: 0 269 1953
Size: 269 1684
PspOMI G'GGCC_C
Cuts at: 0 478 1953
Size: 478 1475
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1495 1527 1953
Size: 1495 32 426
Fragments arranged by size:
1495 426 32
PstI C_TGCA'G
Cuts at: 0 71 644 1559 1953
Size: 71 573 915 394
Fragments arranged by size:
915 573 394 71
PvuII CAG'CTG
Cuts at: 0 918 1554 1560 1953
Size: 918 636 6 393
Fragments arranged by size:
918 636 393 6
SacI G_AGCT'C
Cuts at: 0 1754 1953
Size: 1754 199
SacII CC_GC'GG
Cuts at: 0 1325 1953
Size: 1325 628
SalI G'TCGA_C
Cuts at: 0 409 1953
Size: 409 1544
SanDI GG'GwC_CC
Cuts at: 0 824 1953
Size: 824 1129
SexAI A'CCwGG_T
Cuts at: 0 464 1897 1953
Size: 464 1433 56
Fragments arranged by size:
1433 464 56
SfcI C'TryA_G
Cuts at: 0 67 640 1555 1953
Size: 67 573 915 398
Fragments arranged by size:
915 573 398 67
SmaI CCC'GGG
Cuts at: 0 7 1953
Size: 7 1946
SmlI C'TyrA_G
Cuts at: 0 838 1953
Size: 838 1115
StyI C'CwwG_G
Cuts at: 0 587 1415 1953
Size: 587 828 538
Fragments arranged by size:
828 587 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 387 684 1582 1953
Size: 282 105 297 898 371
Fragments arranged by size:
898 371 297 282 105
TatI w'GTAC_w
Cuts at: 0 651 659 1953
Size: 651 8 1294
Fragments arranged by size:
1294 651 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 357 389 1396 1428 1953
Size: 357 32 1007 32 525
Fragments arranged by size:
1007 525 357 32 32
XmaI C'CCGG_G
Cuts at: 0 5 1953
Size: 5 1948
XmnI GAAnn'nnTTC
Cuts at: 0 1024 1078 1105 1953
Size: 1024 54 27 848
Fragments arranged by size:
1024 848 54 27
ZraI GAC'GTC
Cuts at: 0 932 1953
Size: 932 1021
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AleI
AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbvCI BcgI BciVI BglI BglII Bme1580I BmrI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BsrGI BssHII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NotI NspI PasI PflMI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SalI SanDI SexAI
SfcI SmaI SmlI StyI TaqII TatI TstI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflIII AgeI AhdI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BclI BfrBI BlpI
BmgBI BmtI BplI BpuEI BsaI BsaWI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I DraI DraIII EciI EcoRI EcoRV FseI
FspAI HindIII HpaI KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PvuI RsrII
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI