Restriction map for
pFN21AE4543
(Linear) (Six-base) MAPSORT of: KIEE4543.seq Check: 5339 from: 1 to: 1881
KIEE4543.orf
With 182 enzymes: SgfI *
March 22, 2012 18:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 903 911 1881
Size: 903 8 970
Fragments arranged by size:
970 903 8
AccI GT'mk_AC
Cuts at: 0 1504 1881
Size: 1504 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 667 818 849 1726 1881
Size: 302 365 151 31 877 155
Fragments arranged by size:
877 365 302 155 151 31
AflIII A'CryG_T
Cuts at: 0 962 1881
Size: 962 919
AvaI C'yCGr_G
Cuts at: 0 1232 1881
Size: 1232 649
BanI G'GyrC_C
Cuts at: 0 337 614 1881
Size: 337 277 1267
Fragments arranged by size:
1267 337 277
BanII G_rGCy'C
Cuts at: 0 41 806 1167 1881
Size: 41 765 361 714
Fragments arranged by size:
765 714 361 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 1714 1811 1881
Size: 1714 97 70
Fragments arranged by size:
1714 97 70
BbvCI CC'TCA_GC
Cuts at: 0 603 638 811 1881
Size: 603 35 173 1070
Fragments arranged by size:
1070 603 173 35
BciVI GTATCCnnnnn_n'
Cuts at: 0 73 1881
Size: 73 1808
BclI T'GATC_A
Cuts at: 0 497 1881
Size: 497 1384
BfrBI ATG'CAT
Cuts at: 0 1007 1881
Size: 1007 874
BglII A'GATC_T
Cuts at: 0 1443 1881
Size: 1443 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 1167 1357 1578 1881
Size: 1167 190 221 303
Fragments arranged by size:
1167 303 221 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1881
Size: 793 1088
Bpu10I CC'TnA_GC
Cuts at: 0 603 638 811 1881
Size: 603 35 173 1070
Fragments arranged by size:
1070 603 173 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1390 1881
Size: 928 462 491
Fragments arranged by size:
928 491 462
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1772 1802 1881
Size: 1772 30 79
Fragments arranged by size:
1772 79 30
BseYI C'CCAG_C
Cuts at: 0 1029 1881
Size: 1029 852
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1881
Size: 1114 767
BsiHKAI G_wGCw'C
Cuts at: 0 41 806 1881
Size: 41 765 1075
Fragments arranged by size:
1075 765 41
BsmI GAATG_Cn'
Cuts at: 0 1009 1881
Size: 1009 872
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1171 1881
Size: 1171 710
Bsp1286I G_dGCh'C
Cuts at: 0 41 806 1167 1881
Size: 41 765 361 714
Fragments arranged by size:
765 714 361 41
BspHI T'CATG_A
Cuts at: 0 1835 1881
Size: 1835 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 903 911 1881
Size: 903 8 970
Fragments arranged by size:
970 903 8
BsrBI CCG'CTC
Cuts at: 0 1191 1881
Size: 1191 690
BsrDI GCAATG_nn'
Cuts at: 0 81 1881
Size: 81 1800
BsrFI r'CCGG_y
Cuts at: 0 1037 1881
Size: 1037 844
BsrGI T'GTAC_A
Cuts at: 0 1247 1881
Size: 1247 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1753 1881
Size: 1753 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1764 1881
Size: 1764 117
BstYI r'GATC_y
Cuts at: 0 1443 1881
Size: 1443 438
BstZ17I GTA'TAC
Cuts at: 0 1505 1881
Size: 1505 376
BtgI C'CryG_G
Cuts at: 0 234 1881
Size: 234 1647
ClaI AT'CG_AT
Cuts at: 0 1667 1881
Size: 1667 214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1044 1079 1881
Size: 1044 35 802
Fragments arranged by size:
1044 802 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1561 1881
Size: 1561 320
EaeI y'GGCC_r
Cuts at: 0 2 1536 1881
Size: 2 1534 345
Fragments arranged by size:
1534 345 2
EarI CTCTTCn'nnn_
Cuts at: 0 794 1181 1867 1881
Size: 794 387 686 14
Fragments arranged by size:
794 686 387 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 1881
Size: 1036 845
EcoICRI GAG'CTC
Cuts at: 0 39 804 1881
Size: 39 765 1077
Fragments arranged by size:
1077 765 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 667 793 818 849 1726 1881
Size: 302 365 126 25 31 877 155
Fragments arranged by size:
877 365 302 155 126 31 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 1881
Size: 649 1232
EcoO109I rG'GnC_Cy
Cuts at: 0 642 1626 1881
Size: 642 984 255
Fragments arranged by size:
984 642 255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 1104 1881
Size: 390 714 777
Fragments arranged by size:
777 714 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1430 1462 1701 1733 1881
Size: 1430 32 239 32 148
Fragments arranged by size:
1430 239 148 32 32
HincII GTy'rAC
Cuts at: 0 381 627 1227 1311 1566 1881
Size: 381 246 600 84 255 315
Fragments arranged by size:
600 381 315 255 246 84
HindIII A'AGCT_T
Cuts at: 0 47 890 1366 1881
Size: 47 843 476 515
Fragments arranged by size:
843 515 476 47
HpaI GTT'AAC
Cuts at: 0 1227 1881
Size: 1227 654
MfeI C'AATT_G
Cuts at: 0 521 1881
Size: 521 1360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1701 1881
Size: 450 1251 180
Fragments arranged by size:
1251 450 180
MscI TGG'CCA
Cuts at: 0 4 1538 1881
Size: 4 1534 343
Fragments arranged by size:
1534 343 4
MslI CAynn'nnrTG
Cuts at: 0 433 1762 1840 1881
Size: 433 1329 78 41
Fragments arranged by size:
1329 433 78 41
NaeI GCC'GGC
Cuts at: 0 1039 1881
Size: 1039 842
NcoI C'CATG_G
Cuts at: 0 234 1881
Size: 234 1647
NgoMIV G'CCGG_C
Cuts at: 0 1037 1881
Size: 1037 844
NsiI A_TGCA'T
Cuts at: 0 1009 1881
Size: 1009 872
NspI r_CATG'y
Cuts at: 0 427 966 1011 1881
Size: 427 539 45 870
Fragments arranged by size:
870 539 427 45
PasI CC'CwG_GG
Cuts at: 0 1218 1881
Size: 1218 663
PciI A'CATG_T
Cuts at: 0 962 1881
Size: 962 919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 1881
Size: 586 32 1263
Fragments arranged by size:
1263 586 32
PpuMI rG'GwC_Cy
Cuts at: 0 642 1626 1881
Size: 642 984 255
Fragments arranged by size:
984 642 255
PspXI vC'TCGA_Gb
Cuts at: 0 1232 1881
Size: 1232 649
PstI C_TGCA'G
Cuts at: 0 1016 1881
Size: 1016 865
SacI G_AGCT'C
Cuts at: 0 41 806 1881
Size: 41 765 1075
Fragments arranged by size:
1075 765 41
SapI GCTCTTCn'nnn_
Cuts at: 0 794 1181 1881
Size: 794 387 700
Fragments arranged by size:
794 700 387
ScaI AGT'ACT
Cuts at: 0 870 1881
Size: 870 1011
SexAI A'CCwGG_T
Cuts at: 0 1680 1808 1881
Size: 1680 128 73
Fragments arranged by size:
1680 128 73
SfcI C'TryA_G
Cuts at: 0 176 1012 1881
Size: 176 836 869
Fragments arranged by size:
869 836 176
SmlI C'TyrA_G
Cuts at: 0 907 1232 1369 1881
Size: 907 325 137 512
Fragments arranged by size:
907 512 325 137
SphI G_CATG'C
Cuts at: 0 1011 1881
Size: 1011 870
StyI C'CwwG_G
Cuts at: 0 234 1881
Size: 234 1647
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 1210 1560 1881
Size: 355 855 350 321
Fragments arranged by size:
855 355 350 321
TatI w'GTAC_w
Cuts at: 0 868 1247 1639 1881
Size: 868 379 392 242
Fragments arranged by size:
868 392 379 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1499 1531 1772 1804 1881
Size: 1499 32 241 32 77
Fragments arranged by size:
1499 241 77 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1086 1881
Size: 1086 795
XhoI C'TCGA_G
Cuts at: 0 1232 1881
Size: 1232 649
XmnI GAAnn'nnTTC
Cuts at: 0 941 1881
Size: 941 940
Enzymes that do cut:
AarI AccI AcuI AflIII AvaI BanI BanII BbsI
BbvCI BciVI BclI BfrBI BglII BmrI BpmI Bpu10I
BpuEI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BstZ17I BtgI ClaI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI NaeI NcoI
NgoMIV NsiI NspI PasI PciI PpiI PpuMI PspXI
PstI SacI SapI ScaI SexAI SfcI SmlI SphI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BglI BlpI
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsiEI BsiWI BspEI BssHII BssSI BstBI
BstEII Bsu36I BtgZI BtsI DraI DraIII EagI EcoRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MluI
MspA1I NarI NdeI NheI NotI NruI PacI PflMI
PfoI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XmaI ZraI