Restriction map for
pFN21AE4535
(Linear) (Six-base) MAPSORT of: KIEE4535.seq Check: 6928 from: 1 to: 1806
KIEE4535.orf
With 182 enzymes: SgfI *
March 22, 2012 18:18 ..
AccI GT'mk_AC
Cuts at: 0 201 1806
Size: 201 1605
AclI AA'CG_TT
Cuts at: 0 811 1806
Size: 811 995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 592 674 1077 1806
Size: 104 488 82 403 729
Fragments arranged by size:
729 488 403 104 82
AflIII A'CryG_T
Cuts at: 0 459 1806
Size: 459 1347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 1806
Size: 291 1515
AlwNI CAG_nnn'CTG
Cuts at: 0 228 1806
Size: 228 1578
ApoI r'AATT_y
Cuts at: 0 173 270 896 1129 1411 1466 1500 1806
Size: 173 97 626 233 282 55 34 306
Fragments arranged by size:
626 306 282 233 173 97 55 34
AvaI C'yCGr_G
Cuts at: 0 485 1806
Size: 485 1321
AvrII C'CTAG_G
Cuts at: 0 534 1806
Size: 534 1272
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1095 1128 1806
Size: 1095 33 678
Fragments arranged by size:
1095 678 33
BamHI G'GATC_C
Cuts at: 0 757 1806
Size: 757 1049
BanI G'GyrC_C
Cuts at: 0 529 1806
Size: 529 1277
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1551 1806
Size: 359 1192 255
Fragments arranged by size:
1192 359 255
BclI T'GATC_A
Cuts at: 0 591 1218 1806
Size: 591 627 588
Fragments arranged by size:
627 591 588
BfrBI ATG'CAT
Cuts at: 0 3 26 1395 1806
Size: 3 23 1369 411
Fragments arranged by size:
1369 411 23 3
Bme1580I G_kGCm'C
Cuts at: 0 534 1806
Size: 534 1272
BmrI ACTGGGnnnn_n'
Cuts at: 0 1218 1806
Size: 1218 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1806
Size: 554 1252
BsaI GGTCTCn'nnnn_
Cuts at: 0 1218 1806
Size: 1218 588
BsaAI yAC'GTr
Cuts at: 0 1627 1806
Size: 1627 179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1342 1372 1806
Size: 1342 30 434
Fragments arranged by size:
1342 434 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1806
Size: 698 1108
BsiEI CG_ry'CG
Cuts at: 0 962 1806
Size: 962 844
BsiHKAI G_wGCw'C
Cuts at: 0 1086 1632 1806
Size: 1086 546 174
Fragments arranged by size:
1086 546 174
BsmI GAATG_Cn'
Cuts at: 0 258 1806
Size: 258 1548
Bsp1286I G_dGCh'C
Cuts at: 0 534 1086 1632 1806
Size: 534 552 546 174
Fragments arranged by size:
552 546 534 174
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1504 1806
Size: 1504 302
BstYI r'GATC_y
Cuts at: 0 757 885 1477 1806
Size: 757 128 592 329
Fragments arranged by size:
757 592 329 128
Bsu36I CC'TnA_GG
Cuts at: 0 563 1806
Size: 563 1243
BtgI C'CryG_G
Cuts at: 0 312 962 1369 1806
Size: 312 650 407 437
Fragments arranged by size:
650 437 407 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1711 1806
Size: 1711 95
DraI TTT'AAA
Cuts at: 0 1020 1145 1806
Size: 1020 125 661
Fragments arranged by size:
1020 661 125
DrdI GACnn_nn'nnGTC
Cuts at: 0 237 939 1806
Size: 237 702 867
Fragments arranged by size:
867 702 237
EaeI y'GGCC_r
Cuts at: 0 1372 1806
Size: 1372 434
EarI CTCTTCn'nnn_
Cuts at: 0 611 1434 1806
Size: 611 823 372
Fragments arranged by size:
823 611 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 554 592 674 1077 1806
Size: 104 450 38 82 403 729
Fragments arranged by size:
729 450 403 104 82 38
EcoRI G'AATT_C
Cuts at: 0 173 1806
Size: 173 1633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 916 1806
Size: 916 890
HincII GTy'rAC
Cuts at: 0 1604 1806
Size: 1604 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1806
Size: 982 824
MscI TGG'CCA
Cuts at: 0 1374 1806
Size: 1374 432
MslI CAynn'nnrTG
Cuts at: 0 464 985 1400 1806
Size: 464 521 415 406
Fragments arranged by size:
521 464 415 406
NcoI C'CATG_G
Cuts at: 0 312 1806
Size: 312 1494
NdeI CA'TA_TG
Cuts at: 0 22 1279 1806
Size: 22 1257 527
Fragments arranged by size:
1257 527 22
NsiI A_TGCA'T
Cuts at: 0 5 28 1397 1806
Size: 5 23 1369 409
Fragments arranged by size:
1369 409 23 5
NspI r_CATG'y
Cuts at: 0 63 463 990 1806
Size: 63 400 527 816
Fragments arranged by size:
816 527 400 63
PciI A'CATG_T
Cuts at: 0 459 1806
Size: 459 1347
PflMI CCAn_nnn'nTGG
Cuts at: 0 196 1035 1806
Size: 196 839 771
Fragments arranged by size:
839 771 196
PfoI T'CCnGG_A
Cuts at: 0 714 1158 1806
Size: 714 444 648
Fragments arranged by size:
714 648 444
PsiI TTA'TAA
Cuts at: 0 1271 1806
Size: 1271 535
SapI GCTCTTCn'nnn_
Cuts at: 0 611 1806
Size: 611 1195
SfcI C'TryA_G
Cuts at: 0 519 1806
Size: 519 1287
SphI G_CATG'C
Cuts at: 0 63 1806
Size: 63 1743
SspI AAT'ATT
Cuts at: 0 1151 1689 1806
Size: 1151 538 117
Fragments arranged by size:
1151 538 117
StuI AGG'CCT
Cuts at: 0 1246 1806
Size: 1246 560
StyI C'CwwG_G
Cuts at: 0 312 534 772 1247 1289 1806
Size: 312 222 238 475 42 517
Fragments arranged by size:
517 475 312 238 222 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 517 1030 1240 1806
Size: 517 513 210 566
Fragments arranged by size:
566 517 513 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 411 443 1342 1374 1806
Size: 411 32 899 32 432
Fragments arranged by size:
899 432 411 32 32
XbaI T'CTAG_A
Cuts at: 0 841 1806
Size: 841 965
XmnI GAAnn'nnTTC
Cuts at: 0 723 899 1806
Size: 723 176 907
Fragments arranged by size:
907 723 176
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApoI AvaI
AvrII BaeI BamHI BanI BbsI BclI BfrBI Bme1580I
BmrI BpmI BsaI BsaAI BsaXI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BstAPI BstYI Bsu36I BtgI BtgZI DraI
DrdI EaeI EarI Eco57MI EcoRI FalI HincII MmeI
MscI MslI NcoI NdeI NsiI NspI PciI PflMI
PfoI PsiI SapI SfcI SphI SspI StuI StyI
TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI BanII BbeI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtsI
ClaI CspCI DraIII EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SwaI TatI Tth111I XcmI XhoI XmaI ZraI